1. Formation of a Polycomb-Domain in the Absence of Strong Polycomb Response Elements.

    PLoS Genetics 12(7):e1006200 (2016) PMID 27466807

    Polycomb group response elements (PREs) in Drosophila are DNA-elements that recruit Polycomb proteins (PcG) to chromatin and regulate gene expression. PREs are easily recognizable in the Drosophila genome as strong peaks of PcG-protein binding over discrete DNA fragments; many small but statisti...
  2. Co-regulation of invected and engrailed by a complex array of regulatory sequences in Drosophila.

    Developmental Biology 395(1):131 (2014) PMID 25172431 PMCID PMC4189978

    invected (inv) and engrailed (en) form a gene complex that extends about 115 kb. These two genes encode highly related homeodomain proteins that are co-regulated in a complex manner throughout development. Our dissection of inv/en regulatory DNA shows that most enhancers are spread throughout a ...
  3. Wapl antagonizes cohesin binding and promotes Polycomb-group silencing in Drosophila.

    Development 139(22):4172 (2012) PMID 23034634 PMCID PMC3478686

    Wapl protein regulates binding of the cohesin complex to chromosomes during interphase and helps remove cohesin from chromosomes at mitosis. We isolated a dominant mutation in wapl (wapl(AG)) in a screen for mutations that counteract silencing mediated by an engrailed Polycomb-group response ele...
  4. P-element homing is facilitated by engrailed polycomb-group response elements in Drosophila melanogaster.

    PLoS ONE 7(1):e30437 (2012) PMID 22276200 PMCID PMC3261919

    P-element vectors are commonly used to make transgenic Drosophila and generally insert in the genome in a nonselective manner. However, when specific fragments of regulatory DNA from a few Drosophila genes are incorporated into P-transposons, they cause the vectors to be inserted near the gene f...
  5. Modulation of the activity of a polycomb-group response element in Drosophila by a mutation in the transcriptional activator woc.

    G3: Genes, Genomes, Genetics 1(6):471 (2011) PMID 22384357 PMCID PMC3276158

    Polycomb group response elements (PRE) are cis-regulatory elements that bind Polycomb group proteins. We are studying a 181-bp PRE from the Drosophilaengrailed gene. This PRE causes pairing-sensitive silencing of mini-white in transgenes. Here we show that the 181-bp PRE also represses mini-whit...
  6. Enhancer-promoter communication at the Drosophila engrailed locus.

    Development 136(18):3067 (2009) PMID 19675130 PMCID PMC2730364

    Enhancers are often located many tens of kilobases away from the promoter they regulate, sometimes residing closer to the promoter of a neighboring gene. How do they know which gene to activate? We have used homing P[en] constructs to study the enhancer-promoter communication at the engrailed lo...
  7. Visual mutations reveal opposing effects of illumination on arousal in Drosophila.

    Genetics 178(4):2413 (2008) PMID 18430958 PMCID PMC2323825

    The effect of illumination on alertness can be assessed by comparing the efficacy of an anesthetic under light vs. dark conditions. Results from such tests on wild-type flies and visual mutants demonstrate that, surprisingly, light has both positive and negative influences on arousal. These dual...
  8. Drosophila TRP channels require a protein with a distinctive motif encoded by the inaF locus.

    PNAS 104(45):17730 (2007) PMID 17968007 PMCID PMC2077074

    In both vertebrates and invertebrates, ion channels of the TRP superfamily are known to be influenced by a variety of accessory factors, but the list of interacting proteins is acknowledged to be incomplete. Although previous work showed that Drosophila TRP function is disrupted by mutations in ...
  9. Drosophila fasciclinIIIs Required for the Formation of Odor Memories and for Normal Sensitivity to Alcohol

    Cell 105(6):757 (2001)

    Drosophila fasciclinII ( fasII) mutants perform poorly after olfactory conditioning due to a defect in encoding, stabilizing, or retrieving short-term memories. Performance was rescued by inducing the expression of a normal transgene just before training and immediate testing. ...