1. Rac-mediated Stimulation of Phospholipase Cγ2 Amplifies B Cell Receptor-induced Calcium Signaling.

    Journal of Biological Chemistry 290(28):17056 (2015) PMID 25903139 PMCID PMC4498044

    The Rho GTPase Rac is crucially involved in controlling multiple B cell functions, including those regulated by the B cell receptor (BCR) through increased cytosolic Ca(2+). The underlying molecular mechanisms and their relevance to the functions of intact B cells have thus far remained unknown....
  2. Dab2 inhibits the cholesterol-dependent activation of JNK by TGF-β.

    Molecular Biology of the Cell 25(10):1620 (2014) PMID 24648493 PMCID PMC4019493

    Transforming growth factor-β (TGF-β) ligands activate Smad-mediated and noncanonical signaling pathways in a cell context-dependent manner. Localization of signaling receptors to distinct membrane domains is a potential source of signaling output diversity. The tumor suppressor/endocytic adaptor...
  3. Dab2 inhibits the cholesterol-dependent activation of JNK by TGF-β.

    Molecular Biology of the Cell 25(10):1620 (2014) PMID 24648493 PMCID PMC4019493

    Transforming growth factor-β (TGF-β) ligands activate Smad-mediated and noncanonical signaling pathways in a cell context-dependent manner. Localization of signaling receptors to distinct membrane domains is a potential source of signaling output diversity. The tumor suppressor/endocytic adaptor...
  4. Dab2 inhibits the cholesterol-dependent activation of JNK by TGF-β.

    Molecular Biology of the Cell 25(10):1620 (2014) PMID 24648493 PMCID PMC4019493

    Transforming growth factor-β (TGF-β) ligands activate Smad-mediated and noncanonical signaling pathways in a cell context-dependent manner. Localization of signaling receptors to distinct membrane domains is a potential source of signaling output diversity. The tumor suppressor/endocytic adaptor...
  5. EGF receptor family: twisting targets for improved cancer therapies.

    Growth Factors 32(2):74 (2014) PMID 24641597

    The epidermal growth factor receptor (EGFR) undergoes a conformational change in response to ligand binding. The ligand-induced changes in cell surface aggregation and mobility have a profound effect on the function of all the family members. Ligand also activates the EGFR intracellular kinase, ...
  6. EGF receptor family: twisting targets for improved cancer therapies.

    Growth Factors 32(2):74 (2014) PMID 24641597

    The epidermal growth factor receptor (EGFR) undergoes a conformational change in response to ligand binding. The ligand-induced changes in cell surface aggregation and mobility have a profound effect on the function of all the family members. Ligand also activates the EGFR intracellular kinase, ...
  7. Src-mediated caveolin-1 phosphorylation affects the targeting of active Src to specific membrane sites.

    Molecular Biology of the Cell 24(24):3881 (2013) PMID 24131997 PMCID PMC3861084

    Src interactions with the plasma membrane are an important determinant of its activity. In turn, Src activity modulates its association with the membrane through binding of activated Src to phosphotyrosylated proteins. Caveolin-1 (Cav-1), a major component of caveolae, is a known Src phosphoryla...
  8. Src-mediated caveolin-1 phosphorylation affects the targeting of active Src to specific membrane sites.

    Molecular Biology of the Cell 24(24):3881 (2013) PMID 24131997 PMCID PMC3861084

    Src interactions with the plasma membrane are an important determinant of its activity. In turn, Src activity modulates its association with the membrane through binding of activated Src to phosphotyrosylated proteins. Caveolin-1 (Cav-1), a major component of caveolae, is a known Src phosphoryla...
  9. Dual effects of Ral-activated pathways on p27 localization and TGF-β signaling.

    Molecular Biology of the Cell 24(11):1812 (2013) PMID 23576547 PMCID PMC3667732

    Constitutive activation or overactivation of Ras signaling pathways contributes to epithelial tumorigenesis in several ways, one of which is cytoplasmic mislocalization of the cyclin-dependent kinase inhibitor p27(Kip1) (p27). We previously showed that such an effect can be mediated by activatio...
  10. Dual effects of Ral-activated pathways on p27 localization and TGF-β signaling.

    Molecular Biology of the Cell 24(11):1812 (2013) PMID 23576547 PMCID PMC3667732

    Constitutive activation or overactivation of Ras signaling pathways contributes to epithelial tumorigenesis in several ways, one of which is cytoplasmic mislocalization of the cyclin-dependent kinase inhibitor p27(Kip1) (p27). We previously showed that such an effect can be mediated by activatio...
  11. The role of palmitoylation in regulating Ras localization and function.

    Biochemical Society Transactions 41(1):79 (2013) PMID 23356262

    Ras GTPases are important regulators of pathways controlling proliferation, differentiation and transformation. Three ubiquitously expressed almost identical Ras genes are not functionally redundant; this has been attributed to their distinctive trafficking and localization profiles. A palmitoyl...
  12. The role of palmitoylation in regulating Ras localization and function.

    Biochemical Society Transactions 41(1):79 (2013) PMID 23356262

    Ras GTPases are important regulators of pathways controlling proliferation, differentiation and transformation. Three ubiquitously expressed almost identical Ras genes are not functionally redundant; this has been attributed to their distinctive trafficking and localization profiles. A palmitoyl...
  13. TGF-Beta and Bmp Receptors: Distinct Modes of Oligomeric Interactions and Implications for Signaling

    Biophysical Journal 104(2):612a (2013)

  14. Differential effect of actomyosin relaxation on the dynamic properties of focal adhesion proteins.

    PLoS ONE 8(9):e73549 (2013) PMID 24039980 PMCID PMC3767655

    Treatment of cultured cells with inhibitors of actomyosin contractility induces rapid deterioration of stress fibers, and disassembly of the associated focal adhesions (FAs). In this study, we show that treatment with the Rho kinase inhibitor Y-27632, which blocks actomyosin contractility, induc...
  15. Differential effect of actomyosin relaxation on the dynamic properties of focal adhesion proteins.

    PLoS ONE 8(9):e73549 (2013) PMID 24039980 PMCID PMC3767655

    Treatment of cultured cells with inhibitors of actomyosin contractility induces rapid deterioration of stress fibers, and disassembly of the associated focal adhesions (FAs). In this study, we show that treatment with the Rho kinase inhibitor Y-27632, which blocks actomyosin contractility, induc...
  16. Coated pit-mediated endocytosis of the type I transforming growth factor-β (TGF-β) receptor depends on a di-leucine family signal and is not required for signaling.

    Journal of Biological Chemistry 287(32):26876 (2012) PMID 22707720 PMCID PMC3411024

    The roles of transforming growth factor-β (TGF-β) receptor endocytosis in signaling have been investigated in numerous studies, mainly through the use of endocytosis inhibitory treatments, yielding conflicting results. Two potential sources for these discrepancies were the pleiotropic effects of...
  17. Coated pit-mediated endocytosis of the type I transforming growth factor-β (TGF-β) receptor depends on a di-leucine family signal and is not required for signaling.

    Journal of Biological Chemistry 287(32):26876 (2012) PMID 22707720 PMCID PMC3411024

    The roles of transforming growth factor-β (TGF-β) receptor endocytosis in signaling have been investigated in numerous studies, mainly through the use of endocytosis inhibitory treatments, yielding conflicting results. Two potential sources for these discrepancies were the pleiotropic effects of...
  18. Oligomeric interactions of TGF-β and BMP receptors.

    FEBS Letters 586(14):1885 (2012) PMID 22293501

    Transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) cytokines participate in a multiplicity of ways in the regulation of numerous physiological and pathological processes. Their wide-ranging biological functions are controlled by several mechanisms, including regulation of ...
  19. Oligomeric interactions of TGF-β and BMP receptors

    FEBS Letters 586(14):1885 (2012)

    Transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) cytokines participate in a multiplicity of ways in the regulation of numerous physiological and pathological processes. Their wide-ranging biological functions are controlled by several mechanisms, including regu...
  20. Oligomeric interactions of TGF-β and BMP receptors.

    FEBS Letters 586(14):1885 (2012) PMID 22293501

    Transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) cytokines participate in a multiplicity of ways in the regulation of numerous physiological and pathological processes. Their wide-ranging biological functions are controlled by several mechanisms, including regulation of ...