1. Latent TGF-β binding protein-2 is essential for the development of ciliary zonule microfibrils.

    Human Molecular Genetics 23(21):5672 (2014) PMID 24908666 PMCID PMC4189902

    Latent TGF-β-binding protein-2 (LTBP-2) is an extracellular matrix protein associated with microfibrils. Homozygous mutations in LTBP2 have been found in humans with genetic eye diseases such as congenital glaucoma and microspherophakia, indicating a critical role of the protein in eye developme...
  2. Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans.

    FEBS Letters 587(19):3195 (2013) PMID 23968720 PMCID PMC4029356

    Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acet...
  3. Latent TGF-β binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5.

    PNAS 110(8):2852 (2013) PMID 23382201 PMCID PMC3581912

    Elastic fiber assembly requires deposition of elastin monomers onto microfibrils, the mechanism of which is incompletely understood. Here we show that latent TGF-β binding protein 4 (LTBP-4) potentiates formation of elastic fibers through interacting with fibulin-5, a tropoelastin-binding protei...
  4. In vivo regulation of steroid hormones by the Chst10 sulfotransferase in mouse.

    Journal of Biological Chemistry 288(7):5007 (2013) PMID 23269668 PMCID PMC3576103

    Chst10 adds sulfate to glucuronic acid to form a carbohydrate antigen, HNK-1, in glycoproteins and glycolipids. To determine the role of Chst10 in vivo, we generated systemic Chst10-deficient mutant mice. Although Chst10(-/-) mice were born and grew to adulthood with no gross defects, they were ...
  5. Identification of mono- and disulfated N-acetyl-lactosaminyl Oligosaccharide structures as epitopes specifically recognized by humanized monoclonal antibody HMOCC-1 raised against ovarian cancer.

    Journal of Biological Chemistry 287(9):6592 (2012) PMID 22194598 PMCID PMC3307324

    A humanized monoclonal antibody raised against human ovarian cancer RMG-I cells and designated as HMOCC-1 (Suzuki, N., Aoki, D., Tamada, Y., Susumu, N., Orikawa, K., Tsukazaki, K., Sakayori, M., Suzuki, A., Fukuchi, T., Mukai, M., Kojima-Aikawa, K., Ishida, I., and Nozawa, S. (2004) Gynecol. Onc...
  6. Targeted drug delivery to tumor vasculature by a carbohydrate mimetic peptide.

    PNAS 108(49):19587 (2011) PMID 22114188 PMCID PMC3241764

    Although numerous carbohydrates play significant roles in mammalian cells, carbohydrate-based drug discovery has not been explored due to the technical difficulty of chemically synthesizing complex carbohydrate structures. Previously, we identified a series of carbohydrate mimetic peptides and f...
  7. Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas.

    Journal of Structural Biology 174(3):536 (2011) PMID 21440637

    The spatial distribution of collagen fibrils in the corneal stroma is essential for corneal transparency and is primarily regulated by extrafibrillar proteoglycans, which are multi-functional polymers that interact with hybrid type I/V collagen fibrils. In order to understand more about proteogl...
  8. Trophinin-mediated cell adhesion induces apoptosis of human endometrial epithelial cells through PKC-δ.

    Cell Cycle 10(1):135 (2011) PMID 21191175 PMCID PMC3048080

    Trophinin is an intrinsic membrane protein expressed in trophectoderm cells of embryos and in uterine epithelial cells. Trophinin potentially mediates apical cell adhesion at human embryo implantation sites through trophinin-trophinin binding in these two cell types. Trophinin-mediated cell adhe...
  9. Structural collagen alterations in macular corneal dystrophy occur mainly in the posterior stroma.

    Current Eye Research 35(7):580 (2010) PMID 20597644

    Collagen fibrils in the corneal stroma in macular corneal dystrophy, on average, are more closely spaced than in the normal cornea. This study was conducted to investigate if this occurs uniformly across the stroma or is more prevalent at certain stromal depths. Microbeam synchrotron X-ray fiber...
  10. Structural and biochemical aspects of keratan sulphate in the cornea.

    Experientia. Basel 67(6):891 (2010) PMID 20213925

    Keratan sulphate (KS) is the predominant glycosaminoglycan (GAG) in the cornea of the eye, where it exists in proteoglycan (PG) form. KS-PGs have long been thought to play a pivotal role in the establishment and maintenance of the array of regularly-spaced and uniformly- thin collagen fibrils wh...
  11. Fibulin-4 conducts proper elastogenesis via interaction with cross-linking enzyme lysyl oxidase.

    PNAS 106(45):19029 (2009) PMID 19855011 PMCID PMC2776456

    Great arteries, as well as lungs and skin, contain elastic fibers as important components to maintain their physiological functions. Although recent studies have revealed that a glycoprotein fibulin-4 (FBLN4) is indispensable for the assembly of mature elastic fibers, it remains to be elucidated...
  12. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells.

    PNAS 106(9):3095 (2009) PMID 19218444 PMCID PMC2642669

    Cell surfaces of epithelial cancer are covered by complex carbohydrates, whose structures function in malignancy and metastasis. However, the mechanism underlying carbohydrate-dependent cancer metastasis has not been defined. Previously, we identified a carbohydrate-mimicry peptide designated I-...
  13. Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans.

    Journal of Biological Chemistry 282(41):30085 (2007) PMID 17690104

    Keratan sulfate glycosaminoglycans are among the most abundant carbohydrate components of the cornea and are suggested to play an important role in maintaining corneal extracellular matrix structure. Keratan sulfate carbohydrate chains consist of repeating N-acetyllactosamine disaccharides with ...
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  14. Significant decrease in alpha1,3-linked fucose in association with increase in 6-sulfated N-acetylglucosamine in peripheral lymph node addressin of FucT-VII-deficient mice exhibiting diminished lymphocyte homing.

    Glycobiology 17(3):277 (2007) PMID 17172261

    Lymphocyte homing is mediated by binding of L-selectin on lymphocytes with L-selectin ligands present on high-endothelial venules (HEV) of peripheral and mesenteric lymph nodes. L-selectin ligands are specific O-linked carbohydrates, 6-sulfo sialyl Lewis X, composed of sialylated, fucosylated, a...
  15. Differential immunogold localisation of sulphated and unsulphated keratan sulphate proteoglycans in normal and macular dystrophy cornea using sulphation motif-specific antibodies.

    Histochemistry and Cell Biology 127(1):115 (2007) PMID 16944190

    Keratan sulphate (KS) proteoglycans (PGs) are key molecules in the corneal stroma for tissue organisation and transparency. Macular corneal dystrophy (MCD) is a rare, autosomal recessive disease characterised by disturbances in KS expression. MCD is caused by mutations in CHST6, a gene encoding ...
  16. The Bysl gene product, bystin, is essential for survival of mouse embryos.

    FEBS Letters 580(26):6062 (2006) PMID 17055491 PMCID PMC1764500

    Human bystin is a cytoplasmic protein directly binding to trophinin, a cell adhesion molecule potentially involved in human embryo implantation. The present study shows that bystin is expressed in luminal and glandular epithelia in the mouse uterus at peri-implantation stages. In fertilized embr...
  17. Unusual N-glycan structures in alpha-mannosidase II/IIx double null embryos identified by a systematic glycomics approach based on two-dimensional LC mapping and matrix-dependent selective fragmentation method in MALDI-TOF/TOF mass spectrometry.

    Molecular & Cellular Proteomics 5(11):2146 (2006) PMID 16899540

    alpha-Mannosidase IIx (MX) is an enzyme closely related to alpha-mannosidase II (MII), a key enzyme in N-glycan biosynthesis that catalyzes the first step in conversion of hybrid- to complex-type N-glycans in Golgi apparatus. Recently we generated MII/MX double knock-out mice and found that doub...
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  18. Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase.

    PNAS 103(36):13333 (2006) PMID 16938851 PMCID PMC1569164

    Matrix assembly and homeostasis in collagen-rich tissues are mediated by interactions with proteoglycans (PGs) substituted with sulfated glycosaminoglycans (GAGs). The major GAG in cornea is keratan sulfate (KS), which is N-linked to one of three PG core proteins. To ascertain the importance of ...
  19. Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice.

    PNAS 103(24):8983 (2006) PMID 16754854 PMCID PMC1474017

    Many proteins synthesized through the secretory pathway receive posttranslational modifications, including N-glycosylation. alpha-Mannosidase II (MII) is a key enzyme converting precursor high-mannose-type N-glycans to matured complex-type structures. Previous studies showed that MII-null mice s...
  20. N-Glycan structure analysis using lectins and an alpha-mannosidase activity assay.

    Methods in Enzymology 416:304 (2006) PMID 17113875

    Alpha-mannosidase IIx (MX) and alpha-mannosidase II (MII) are homologous enzymes whose critical roles in N-glycan processing were established in large part by analysis of the MII/MX double-knockout mouse. To analyze the structures of N-glycans synthesized in the mutant mice, we employed lectin b...