1. Optimized chemical proteomics assay for kinase inhibitor profiling.

    Journal of Proteome Research 14(3):1574 (2015) PMID 25660469

    Solid supported probes have proven to be an efficient tool for chemical proteomics. The kinobeads technology features kinase inhibitors covalently attached to Sepharose for affinity enrichment of kinomes from cell or tissue lysates. This technology, combined with quantitative mass spectrometry, ...
  2. Proteomics. Tracking cancer drugs in living cells by thermal profiling of the proteome.

    Science 346(6205):1255784 (2014) PMID 25278616

    The thermal stability of proteins can be used to assess ligand binding in living cells. We have generalized this concept by determining the thermal profiles of more than 7000 proteins in human cells by means of mass spectrometry. Monitoring the effects of small-molecule ligands on the profiles d...
  3. Tracking cancer drugs in living cells by thermal profiling of the proteome.

    Science 346(6205):1255784 (2014) PMID 25278616

    The thermal stability of proteins can be used to assess ligand binding in living cells. We have generalized this concept by determining the thermal profiles of more than 7000 proteins in human cells by means of mass spectrometry. Monitoring the effects of small-molecule ligands on the profiles d...
  4. Plant Cytokinesis Is Orchestrated by the Sequential Action of the TRAPPII and Exocyst Tethering Complexes

    Developmental Cell 29(5):607 (2014)

    Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood. In this study, we monitor the Ara...
  5. Plant cytokinesis is orchestrated by the sequential action of the TRAPPII and exocyst tethering complexes.

    Developmental Cell 29(5):607 (2014) PMID 24882377

    Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood. In this study, we monitor the Arabidopsis t...
  6. Plant cytokinesis is orchestrated by the sequential action of the TRAPPII and exocyst tethering complexes.

    Developmental Cell 29(5):607 (2014) PMID 24882377

    Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood. In this study, we monitor the Arabidopsis t...
  7. DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.

    Nature Methods 10(10):989 (2013) PMID 23975139

    We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on ...
  8. DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.

    Nature Methods 10(10):989 (2013) PMID 23975139

    We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on ...
  9. MScDB: a mass spectrometry-centric protein sequence database for proteomics.

    Journal of Proteome Research 12(6):2386 (2013) PMID 23627461

    Protein sequence databases are indispensable tools for life science research including mass spectrometry (MS)-based proteomics. In current database construction processes, sequence similarity clustering is used to reduce redundancies in the source data. Albeit powerful, it ignores the peptide-ce...
  10. MScDB: a mass spectrometry-centric protein sequence database for proteomics.

    Journal of Proteome Research 12(6):2386 (2013) PMID 23627461

    Protein sequence databases are indispensable tools for life science research including mass spectrometry (MS)-based proteomics. In current database construction processes, sequence similarity clustering is used to reduce redundancies in the source data. Albeit powerful, it ignores the peptide-ce...
  11. Phosphorylation site localization in peptides by MALDI MS/MS and the Mascot Delta Score.

    Analytical and Bioanalytical Chemistry 402(1):249 (2012) PMID 22038583

    Owing to its broad biological significance, the large-scale analysis of protein phosphorylation is more and more getting into the focus of proteomic research. Thousands of phosphopeptides can nowadays be identified using state-of-the-art tandem mass spectrometers in conjunction with sequence dat...
  12. Phosphorylation site localization in peptides by MALDI MS/MS and the Mascot Delta Score.

    Analytical and Bioanalytical Chemistry 402(1):249 (2012) PMID 22038583

    Owing to its broad biological significance, the large-scale analysis of protein phosphorylation is more and more getting into the focus of proteomic research. Thousands of phosphopeptides can nowadays be identified using state-of-the-art tandem mass spectrometers in conjunction with sequence dat...