1. Molecular identification of tuliposide B-converting enzyme: a lactone-forming carboxylesterase from the pollen of tulip.

    Plant Journal 83(2):252 (2015) PMID 25997073

    6-Tuliposides A (PosA) and B (PosB), which are the major secondary metabolites in tulip (Tulipa gesneriana), are enzymatically converted to the antimicrobial lactonized aglycons, tulipalins A (PaA) and B (PaB), respectively. We recently identified a PosA-converting enzyme (TCEA) as the first rep...
  2. A protocol for axenic liquid cell cultures of a woody leguminous mangrove, Caesalpinia crista, and their amino acids profiling.

    Natural product communications 10(5):755 (2015) PMID 26058151

    Callus induction, maintenance and protoplast cultures were achieved from immature seeds of a woody leguminous mangrove, Caesalpinia crista. Axenic cultures were possible during 1.5 months of pod storage in 0.1% benzalkonium chloride solution. Callus induction was achieved using 1 mL liquid mediu...
  3. A stepwise protocol for induction and selection of prominent coniferous cell cultures for the production of β-thujaplicin.

    Natural product communications 10(5):783 (2015) PMID 26058158

    In order to demonstrate the potential of plant cell culture systems to produce a target natural bioactive compound, we proposed a stepwise protocol for β-thujaplicin production as follows. 1. Induction phase: Characteristics of callus cultures originating from newly flushed shoots of 10 conifer ...
  4. The biosynthetic activities of primary and secondary metabolites in suspension cultures of Aquilaria microcarpa.

    Natural product communications 10(5):779 (2015) PMID 26058157

    Two types of suspension-cultured Aquilaria microcarpa cells, friable and aggregated, were selectively generated. The biosynthetic activities of primary and secondary metabolites in target cells were detected using laser scanning microscopy (LSM) imaging with diphenylboric acid 2-amino ethyl este...
  5. Plant cell, tissue and organ culture: the most flexible foundations for plant metabolic engineering applications.

    Natural product communications 10(5):815 (2015) PMID 26058164

    Significant advances in plant cell, tissue and organ culture (PCTOC) have been made in the last five decades. PCTOC is now thought to be the underlying technique for understanding general or specific biological functions of the plant kingdom, and it is one of the most flexible foundations for mo...
  6. Environmentally benign process for the preparation of antimicrobial α-methylene-β-hydroxy-γ-butyrolactone (tulipalin B) from tulip biomass.

    Bioscience, Biotechnology, and Biochemistry 79(1):25 (2015) PMID 25126881

    Tulipalin B (α-methylene-β-hydroxy-γ-butyrolactone, PaB) is an antimicrobial natural product occurring in tulip (Tulipa gesneriana). PaB is directly formed from the precursor glucose ester 6-tuliposide B (PosB) by endogenous Pos-converting enzyme (TCE). Despite the potential usefulness of antiba...
  7. Characterization of giant spheroplasts generated from the aerobic anoxygenic photosynthetic marine bacterium Roseobacter litoralis.

    Journal of General and Applied Microbiology 61(2):44 (2015) PMID 26018500

    We generated and characterized giant spheroplasts from the aerobic anoxygenic photosynthetic marine bacterium Roseobacter litoralis. The giant spheroplasts contained vacuole-like structures within the cells, mainly consisting of a single membrane. The in vivo absorption spectrum of the giant sph...
  8. The co-occurrence of two pyridine alkaloids, mimosine and trigonelline, in Leucaena leucocephala.

    Zeitschrift fuer Naturforschung. Section C 69(3-4):124 (2014) PMID 24873033

    Leucaena leucocephala is a nitrogen-fixing tropical leguminous tree that produces two pyridine alkaloids, i. e. mimosine [beta-(3-hydroxy-4-pyridon-1-yl)-L-alanine] and trigonelline (1-methylpyridinium-3-carboxylate). Mimosine has been detected in leaves, flowers, pods, seeds, and roots, and it ...
  9. Two new β-glucosidases from ethanol-fermenting fungus Mucor circinelloides NBRC 4572: enzyme purification, functional characterization, and molecular cloning of the gene.

    Applied Microbiology and Biotechnology 97(23):10045 (2013) PMID 24061417

    Two β-glucosidases (BGLs 1 and 2) were purified to homogeneity from the extracellular enzyme preparations of the ethanol-fermenting Mucor circinelloides NBRC 4572 statically grown on rice straw. BGLs 1 and 2 are monomeric glycoproteins whose apparent molecular masses (Ms) are around 78 kDa, whic...
  10. Molecular diversity of tuliposide A-converting enzyme in the tulip.

    Bioscience, Biotechnology, and Biochemistry 77(5):1042 (2013) PMID 23649245

    Tuliposide A-converting enzyme (TCEA) catalyzes the conversion of 6-tuliposide A to its lactonized aglycon, tulipalin A, in the tulip (Tulipa gesneriana). The TgTCEA gene, isolated previously from petals, was transcribed in all tulip tissues but not in the bulbs despite the presence of TCEA acti...
  11. A novel lactone-forming carboxylesterase: molecular identification of a tuliposide A-converting enzyme in tulip.

    Plant Physiology 159(2):565 (2012) PMID 22474185 PMCID PMC3375925

    Tuliposides, the glucose esters of 4-hydroxy-2-methylenebutanoate and 3,4-dihydroxy-2-methylenebutanoate, are major secondary metabolites in tulip (Tulipa gesneriana). Their lactonized aglycons, tulipalins, function as defensive chemicals due to their biological activities. We recently found tha...
  12. A novel xylogenic suspension culture model for exploring lignification in Phyllostachys bamboo.

    Plant Methods 8(1):40 (2012) PMID 22978662 PMCID PMC3462127

    Some prominent cultured plant cell lines, such as the BY-2 cell line of tobacco (Nicotiana tabacum cv. 'Bright Yellow 2') and the T87 cell line of Arabidopsis (Arabidopsis thaliana L. Heynh., ecotype Columbia) are used as model plant cells. These suspension cell culture systems are highly applic...
  13. Methyl jasmonate-induced enhancement of expression activity of Am-FaPS-1, a putative farnesyl diphosphate synthase gene from Aquilaria microcarpa.

    Journal of Natural Medicines 65(1):194 (2011) PMID 20686864

    A cDNA clone, designated Am-FaPS-1 (1310 bp), was isolated from callus culture derived from the leaf tissues of Aquilaria microcarpa. This gene contains an open reading frame encoding the protein of 342 amino acid residues with high homology to farnesyl diphosphate synthase from various plant so...
  14. Development of bioethanol production by consolidated bioprocess usingM.javanicus

    Journal of Biotechnology 150:158 (2010)

  15. Distribution and biosynthesis of theanine in Theaceae plants.

    Plant Physiology and Biochemistry 48(1):70 (2010) PMID 19828327

    The theanine content of the leaves of 27 species or varieties of Theaceae plants was investigated. Theanine was present in 21 species or varieties, but in much lower amounts (<0.2 mumol/g fresh weight) than the quantity detected in Camellia sinensis var. sinensis. The major free amino acids in l...
  16. Transcriptional activation of putative calmodulin genes am-cam-1 and am-cam-2 from Aquilaria microcarpa, in response to external stimuli.

    Biological & Pharmaceutical Bulletin 33(11):1911 (2010) PMID 21048321

    A homology-based cloning strategy yielded two cDNA clones designated Am-cam-1 and Am-cam-2, presumably encoding calmodulin protein from a callus culture derived from the leaf tissues of Aquilaria microcarpa. An appreciable increase in the transcriptional activity of Am-cam-1 was reproducibly obs...
  17. Purification and characterization of a tuliposide-converting enzyme from bulbs of Tulipa gesneriana.

    Bioscience, Biotechnology, and Biochemistry 73(8):1895 (2009) PMID 19661715

    An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral...
  18. A simple shoot multiplication procedure using internode explants, and its application for particle bombardment and Agrobacterium-mediated transformation in watercress.

    Journal of Plant Research 122(4):455 (2009) PMID 19308313

    A shoot multiplication system derived from internode explants was investigated with the aim of improving genetic characteristics of watercress (Nasturtium officinale R. Br.). Internodes of ca. 1 cm excised from in vitro stock shoot culture were placed on half-strength Murashige and Skoog (MS) me...
  19. Possibility for selective accumulation of polyphenolics in tissue cultures of senno (Lychnis senno Siebold et Zucc.).

    Natural product communications 4(3):377 (2009) PMID 19413116

    Senno (Lychnis senno Siebold et Zucc.), a traditional ornamental plant in Japan had been used as a crude drug acting as natural blood thinners. Since tissue culture protocols have been established, we analyzed polyphenol accumulation profiles in shoot culture, multiple shoot culture, and callus ...
  20. Ethylamine content and theanine biosynthesis in different organs of Camellia sinensis seedlings.

    Zeitschrift fuer Naturforschung. Section C 64(5-6):387 (2009) PMID 19678543

    We examined the distribution of ethylamine, glutamic acid and alanine, which are utilized in theanine biosynthesis, and other major amino acids in leaves, stems, cotyledons and roots of 6-week-old tea seedlings. Ethylamine and glutamic acid, which are substrates of theanine synthetase, were dist...