1. Proteome rearrangements after auditory learning: high-resolution profiling of synapse-enriched protein fractions from mouse brain.

    Journal of Neurochemistry 138(1):124 (2016) PMID 27062398

    Learning and memory processes are accompanied by rearrangements of synaptic protein networks. While various studies have demonstrated the regulation of individual synaptic proteins during these processes, much less is known about the complex regulation of synaptic proteomes. Recently, we reporte...
  2. An S-Acylation Switch of Conserved G Domain Cysteines Is Required for Polarity Signaling by ROP GTPases

    Current Biology 25(21):2875 (2015)

  3. Plant cytokinesis: a tale of membrane traffic and fusion.

    Biochemical Society Transactions 43(1):73 (2015) PMID 25619248

    Cytokinesis separates the forming daughter cells. Higher plants have lost the ability to constrict the plasma membrane (PM) in the division plane. Instead, trans-Golgi network (TGN)-derived membrane vesicles are targeted to the centre of the division plane and generate, by homotypic fusion, the ...
  4. Delivery of endocytosed proteins to the cell-division plane requires change of pathway from recycling to secretion.

    eLife 3:e02131 (2014) PMID 24714496 PMCID PMC3979144

    Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify ...
  5. Plant secretome - from cellular process to biological activity.

    Biochimica et Biophysica Acta 1834(11):2429 (2013) PMID 23557863

    Recent studies suggest that plants secrete a large number of proteins and peptides into the extracellular space. Secreted proteins play a crucial role in stress response, communication and development of organisms. Here we review the current knowledge of the secretome of more than ten plant spec...
  6. Radiolabeling of [18F]altanserin - a microfluidic approach.

    Nuclear Medicine and Biology 39(7):1087 (2012) PMID 22633218

    Our aim was the optimization of radiochemical parameters for the microfluidic preparation of [(18)F]altanserin. The four main parameters evaluated were (1) precursor concentration, (2) reaction temperature, (3) bolus flow rate through the microreactor and (4) bolus volume. For the determination ...
  7. Polarized cell growth in Arabidopsis requires endosomal recycling mediated by GBF1-related ARF exchange factors.

    Nature Cell Biology 14(1):80 (2012) PMID 22138577

    Polarized tip growth is a fundamental cellular process in many eukaryotic organisms, mediating growth of neuronal axons and dendrites or fungal hyphae. In plants, pollen and root hairs are cellular model systems for analysing tip growth. Cell growth depends on membrane traffic. The regulation of...
  8. Elucidating the principles of the molecular organization of heteropolymeric tight junction strands.

    Experientia. Basel 68(23):3903 (2011) PMID 21533891 PMCID PMC4336547

    Paracellular barrier properties of tissues are mainly determined by the composition of claudin heteropolymers. To analyze the molecular organization of tight junctions (TJ), we investigated the ability of claudins (Cld) to form homo- and heteromers. Cld1, -2, -3, -5, and -12 expressed in cerebra...
  9. Antibiotic resistance in sputum isolates of Streptococcus pneumoniae in chronic obstructive pulmonary disease is related to antibiotic exposure.

    Journal of Chronic Obstructive Pulmonary Disease 7(5):337 (2010) PMID 20854048

    Streptococcus pneumoniae (S. pneumoniae) is recovered from sputum of patients with chronic obstructive pulmonary disease (COPD) during stable disease and exacerbations. In patients with community acquired pneumonia, antibiotic exposure in the prior 3-6 months is associated with recovery of antib...
  10. An S-acylation switch of conserved G domain cysteines is required for polarity signaling by ROP GTPases.

    Current Biology 20(10):914 (2010) PMID 20451389

    Rho GTPases are master regulators of cell polarity. For their function, Rhos must associate with discrete plasma membrane domains. Rho of Plants (ROPs) or RACs comprise a single family. Prenylation and S-acylation of hypervariable domain cysteines of Ras and Rho GTPases are required for their fu...
  11. Endocytic and secretory traffic in Arabidopsis merge in the trans-Golgi network/early endosome, an independent and highly dynamic organelle.

    Plant Cell 22(4):1344 (2010) PMID 20435907 PMCID PMC2879741

    Plants constantly adjust their repertoire of plasma membrane proteins that mediates transduction of environmental and developmental signals as well as transport of ions, nutrients, and hormones. The importance of regulated secretory and endocytic trafficking is becoming increasingly clear; howev...
  12. Role of the GNOM gene in Arabidopsis apical-basal patterning--From mutant phenotype to cellular mechanism of protein action.

    European Journal of Cell Biology 89(2-3):138 (2010) PMID 20036441

    How the apical-basal axis of polarity is established in embryogenesis is still a mystery in plant development. This axis appeared specifically compromised by mutations in the Arabidopsis GNOM gene. Surprisingly, GNOM encodes an ARF guanine-nucleotide exchange factor (ARF-GEF) that regulates the ...
  13. Post-Golgi traffic in plants.

    Traffic 10(7):819 (2009) PMID 19416470

    Secretory and endocytic traffic through the post-Golgi endomembrane system regulates the abundance of plasma-membrane proteins such as receptors, transporters and ion channels, modulating the ability of a cell to communicate with its neighbours and to adapt to a changing environment. The major p...
  14. Intrinsic tissue fluorescence in an organotypic perfusion culture of the porcine ocular fundus exposed to blue light and free radicals.

    Graefe's Archive for Clinical and Experimental ... 246(7):979 (2008) PMID 18351374

    A wide variety of pathological pathways may result in age-related macular degeneration. Because of its complexity, there is no comprehensive model of the disease yet. One key feature is the accumulation of the autofluorescent pigment lipofuscin in the retinal pigment epithelium (RPE). Thus, we d...
  15. Plant Cytokinesis Requires De Novo Secretory Trafficking but Not Endocytosis

    Current Biology 17(23):2047 (2007)

    During plant cytokinesis membrane vesicles are efficiently delivered to the cell-division plane, where they fuse with one another to form a laterally expanding cell plate [1, 2]. These membrane vesicles were generally believed to originate from Golgi stacks. Recently, however, it was p...
  16. Functional diversification of closely related ARF-GEFs in protein secretion and recycling.

    Nature 448(7152):488 (2007) PMID 17653190

    Guanine-nucleotide exchange factors on ADP-ribosylation factor GTPases (ARF-GEFs) regulate vesicle formation in time and space by activating ARF substrates on distinct donor membranes. Mammalian GBF1 (ref. 2) and yeast Gea1/2 (ref. 3) ARF-GEFs act at Golgi membranes, regulating COPI-coated vesic...
  17. Retinal pigment epithelium cell damage by A2-E and its photo-derivatives.

    Molecular Vision 12:1348 (2006) PMID 17110917

    A2-E, a major component of the retinal pigment epithelium (RPE) lipofuscin, is a compound that can neither be degraded by nor eliminated from cells and is toxic as well as phototoxic to the cells. Illumination of A2-E with short wavelength light results in isomerization, photooxidation, as well ...
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  18. Sodium fluorescein as a retinal pH indicator?

    Physiological Measurement 26(4):N9 (2005) PMID 15886427

    Retinal neovascularization is a symptom associated with various diseases revealing ocular fundus manifestation. Often, these neovascularizations originate from retinal hypoxia. A concomitant phenomenon of hypoxia is acidosis. To recognise this would permit the identification and treatment of hyp...
  19. Partial loss-of-function alleles reveal a role for GNOM in auxin transport-related, post-embryonic development of Arabidopsis.

    Development 131(2):389 (2004) PMID 14681187

    The Arabidopsis GNOM gene encodes an ARF GDP/GTP exchange factor involved in embryonic axis formation and polar localisation of the auxin efflux regulator PIN1. To examine whether GNOM also plays a role in post-embryonic development and to clarify its involvement in auxin transport, we have char...
  20. The Arabidopsis HINKEL Gene Encodes a Kinesin-Related Protein Involved in Cytokinesis and Is Expressed in a Cell Cycle-Dependent Manner

    Current Biology 12(2):153 (2002)

    Plant cytokinesis starts in the center of the division plane, with vesicle fusion generating a new membrane compartment, the cell plate, that subsequently expands laterally by continuous fusion of newly arriving vesicles to its margin [1–4]. Targeted delivery of vesicles is assisted by...