1. Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis.

    Cell 161(2):264 (2015) PMID 25860609

    The gastrointestinal (GI) tract contains much of the body's serotonin (5-hydroxytryptamine, 5-HT), but mechanisms controlling the metabolism of gut-derived 5-HT remain unclear. Here, we demonstrate that the microbiota plays a critical role in regulating host 5-HT. Indigenous spore-forming bacter...
  2. Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis.

    Cell 161(2):264 (2015) PMID 25860609 PMCID PMC4393509

    The gastrointestinal (GI) tract contains much of the body's serotonin (5-hydroxytryptamine, 5-HT), but mechanisms controlling the metabolism of gut-derived 5-HT remain unclear. Here, we demonstrate that the microbiota plays a critical role in regulating host 5-HT. Indigenous spore-forming bacter...
  3. Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis.

    Cell 161(2):264 (2015) PMID 25860609

    The gastrointestinal (GI) tract contains much of the body's serotonin (5-hydroxytryptamine, 5-HT), but mechanisms controlling the metabolism of gut-derived 5-HT remain unclear. Here, we demonstrate that the microbiota plays a critical role in regulating host 5-HT. Indigenous spore-forming bacter...
  4. Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis.

    Cell 161(2):264 (2015) PMID 25860609 PMCID PMC4393509

    The gastrointestinal (GI) tract contains much of the body's serotonin (5-hydroxytryptamine, 5-HT), but mechanisms controlling the metabolism of gut-derived 5-HT remain unclear. Here, we demonstrate that the microbiota plays a critical role in regulating host 5-HT. Indigenous spore-forming bacter...
  5. The pumping lid: investigating multi-material 3D printing for equipment-free, programmable generation of positive and negative pressures for microfluidic applications.

    Lab On a Chip 14(24):4616 (2014) PMID 25231706

    Equipment-free pumping is a challenging problem and an active area of research in microfluidics, with applications for both laboratory and limited-resource settings. This paper describes the pumping lid method, a strategy to achieve equipment-free pumping by controlled generation of pressure. Pr...
  6. Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering.

    Nature Nanotechnology 9(12):969 (2014) PMID 25466541

    Rapid progress in identifying disease biomarkers has increased the importance of creating high-performance detection technologies. Over the last decade, the design of many detection platforms has focused on either the nano or micro length scale. Here, we review recent strategies that combine nan...
  7. Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness.

    Nature 514(7524):638 (2014) PMID 25274297 PMCID PMC4214913

    Systemic infection induces conserved physiological responses that include both resistance and 'tolerance of infection' mechanisms. Temporary anorexia associated with an infection is often beneficial, reallocating energy from food foraging towards resistance to infection or depriving pathogens of...
  8. Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness.

    Nature 514(7524):638 (2014) PMID 25274297 PMCID PMC4214913

    Systemic infection induces conserved physiological responses that include both resistance and 'tolerance of infection' mechanisms. Temporary anorexia associated with an infection is often beneficial, reallocating energy from food foraging towards resistance to infection or depriving pathogens of...
  9. Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness.

    Nature 514(7524):638 (2014) PMID 25274297 PMCID PMC4214913

    Systemic infection induces conserved physiological responses that include both resistance and 'tolerance of infection' mechanisms. Temporary anorexia associated with an infection is often beneficial, reallocating energy from food foraging towards resistance to infection or depriving pathogens of...
  10. Digital, ultrasensitive, end-point protein measurements with large dynamic range via Brownian trapping with drift.

    Journal of the American Chemical Society 136(42):14662 (2014) PMID 25289692

    This communication shows that the concept of Brownian trapping with drift can be applied to improve quantitative molecular measurements. It has the potential to combine the robustness of end-point spatially resolved readouts, the ultrasensitivity of digital single-molecule measurements, and the ...
  11. Digital biology and chemistry.

    Lab On a Chip 14(17):3225 (2014) PMID 24889331

    This account examines developments in "digital" biology and chemistry within the context of microfluidics, from a personal perspective. Using microfluidics as a frame of reference, we identify two areas of research within digital biology and chemistry that are of special interest: (i) the study ...
  12. Individually addressable arrays of replica microbial cultures enabled by splitting SlipChips.

    Integrative Biology 6(8):796 (2014) PMID 24953827 PMCID PMC4131746

    Isolating microbes carrying genes of interest from environmental samples is important for applications in biology and medicine. However, this involves the use of genetic assays that often require lysis of microbial cells, which is not compatible with the goal of obtaining live cells for isolatio...
  13. Individually addressable arrays of replica microbial cultures enabled by splitting SlipChips.

    Integrative Biology 6(8):796 (2014) PMID 24953827 PMCID PMC4131746

    Isolating microbes carrying genes of interest from environmental samples is important for applications in biology and medicine. However, this involves the use of genetic assays that often require lysis of microbial cells, which is not compatible with the goal of obtaining live cells for isolatio...
  14. Individually addressable arrays of replica microbial cultures enabled by splitting SlipChips.

    Integrative Biology 6(8):796 (2014) PMID 24953827 PMCID PMC4131746

    Isolating microbes carrying genes of interest from environmental samples is important for applications in biology and medicine. However, this involves the use of genetic assays that often require lysis of microbial cells, which is not compatible with the goal of obtaining live cells for isolatio...
  15. Measuring fate and rate of single-molecule competition of amplification and restriction digestion, and its use for rapid genotyping tested with hepatitis C viral RNA.

    Angewandte Chemie. International edition in Eng... 53(31):8088 (2014) PMID 24889060 PMCID PMC4116457

    We experimentally monitored, at the single-molecule level, the competition among reverse transcription, exponential amplification (RT-LAMP), and linear degradation (restriction enzymes) starting with hepatitis C viral RNA molecules. We found significant heterogeneity in the rate of single-molecu...
  16. Measuring fate and rate of single-molecule competition of amplification and restriction digestion, and its use for rapid genotyping tested with hepatitis C viral RNA.

    Angewandte Chemie. International edition in Eng... 53(31):8088 (2014) PMID 24889060 PMCID PMC4116457

    We experimentally monitored, at the single-molecule level, the competition among reverse transcription, exponential amplification (RT-LAMP), and linear degradation (restriction enzymes) starting with hepatitis C viral RNA molecules. We found significant heterogeneity in the rate of single-molecu...
  17. Gene-targeted microfluidic cultivation validated by isolation of a gut bacterium listed in Human Microbiome Project's Most Wanted taxa.

    PNAS 111(27):9768 (2014) PMID 24965364 PMCID PMC4103313

    This paper describes a microfluidics-based workflow for genetically targeted isolation and cultivation of microorganisms from complex clinical samples. Data sets from high-throughput sequencing suggest the existence of previously unidentified bacterial taxa and functional genes with high biomedi...
  18. Gene-targeted microfluidic cultivation validated by isolation of a gut bacterium listed in Human Microbiome Project's Most Wanted taxa.

    PNAS 111(27):9768 (2014) PMID 24965364 PMCID PMC4103313

    This paper describes a microfluidics-based workflow for genetically targeted isolation and cultivation of microorganisms from complex clinical samples. Data sets from high-throughput sequencing suggest the existence of previously unidentified bacterial taxa and functional genes with high biomedi...
  19. A microfluidic device for dry sample preservation in remote settings.

    Lab On a Chip 13(22):4331 (2013) PMID 24056744 PMCID PMC3851311

    This paper describes a microfluidic device for dry preservation of biological specimens at room temperature that incorporates chemical stabilization matrices. Long-term stabilization of samples is crucial for remote medical analysis, biosurveillance, and archiving, but the current paradigm for t...
  20. Increased robustness of single-molecule counting with microfluidics, digital isothermal amplification, and a mobile phone versus real-time kinetic measurements.

    Analytical chemistry 85(22):11129 (2013) PMID 24199852 PMCID PMC3924768

    Quantitative bioanalytical measurements are commonly performed in a kinetic format and are known to not be robust to perturbation that affects the kinetics itself or the measurement of kinetics. We hypothesized that the same measurements performed in a "digital" (single-molecule) format would sh...