1. Remarkably Divergent Regions Punctuate the Genome Assembly of the Caenorhabditis elegans Hawaiian Strain CB4856.

    Genetics 200(3):975 (2015) PMID 25995208 PMCID PMC4512556

    The Hawaiian strain (CB4856) of Caenorhabditis elegans is one of the most divergent from the canonical laboratory strain N2 and has been widely used in developmental, population, and evolutionary studies. To enhance the utility of the strain, we have generated a draft sequence of the CB4856 geno...
  2. Regulatory analysis of the C. elegans genome with spatiotemporal resolution.

    Nature 512(7515):400 (2014) PMID 25164749

    Discovering the structure and dynamics of transcriptional regulatory events in the genome with cellular and temporal resolution is crucial to understanding the regulatory underpinnings of development and disease. We determined the genomic distribution of binding sites for 92 transcription factor...
  3. Comparative analysis of regulatory information and circuits across distant species.

    Nature 512(7515):453 (2014) PMID 25164757 PMCID PMC4336544

    Despite the large evolutionary distances between metazoan species, they can show remarkable commonalities in their biology, and this has helped to establish fly and worm as model organisms for human biology. Although studies of individual elements and factors have explored similarities in gene r...
  4. Comparative analysis of regulatory information and circuits across distant species.

    Nature 512(7515):453 (2014) PMID 25164757 PMCID PMC4336544

    Despite the large evolutionary distances between metazoan species, they can show remarkable commonalities in their biology, and this has helped to establish fly and worm as model organisms for human biology. Although studies of individual elements and factors have explored similarities in gene r...
  5. Comparative analysis of regulatory information and circuits across distant species.

    Nature 512(7515):453 (2014) PMID 25164757

    Despite the large evolutionary distances between metazoan species, they can show remarkable commonalities in their biology, and this has helped to establish fly and worm as model organisms for human biology. Although studies of individual elements and factors have explored similarities in gene r...
  6. Comparative analysis of regulatory information and circuits across distant species.

    Nature 512(7515):453 (2014) PMID 25164757

    Despite the large evolutionary distances between metazoan species, they can show remarkable commonalities in their biology, and this has helped to establish fly and worm as model organisms for human biology. Although studies of individual elements and factors have explored similarities in gene r...
  7. Comparative analysis of regulatory information and circuits across distant species.

    Nature 512(7515):453 (2014) PMID 25164757 PMCID PMC4336544

    Despite the large evolutionary distances between metazoan species, they can show remarkable commonalities in their biology, and this has helped to establish fly and worm as model organisms for human biology. Although studies of individual elements and factors have explored similarities in gene r...
  8. Comparative analysis of regulatory information and circuits across distant species.

    Nature 512(7515):453 (2014) PMID 25164757 PMCID PMC4336544

    Despite the large evolutionary distances between metazoan species, they can show remarkable commonalities in their biology, and this has helped to establish fly and worm as model organisms for human biology. Although studies of individual elements and factors have explored similarities in gene r...
  9. Regulatory analysis of the C. elegans genome with spatiotemporal resolution.

    Nature 512(7515):400 (2014) PMID 25164749

    Discovering the structure and dynamics of transcriptional regulatory events in the genome with cellular and temporal resolution is crucial to understanding the regulatory underpinnings of development and disease. We determined the genomic distribution of binding sites for 92 transcription factor...
  10. Isolation of specific neurons from C. elegans larvae for gene expression profiling.

    PLoS ONE 9(11):e112102 (2014) PMID 25372608 PMCID PMC4221280

    The simple and well-described structure of the C. elegans nervous system offers an unprecedented opportunity to identify the genetic programs that define the connectivity and function of individual neurons and their circuits. A correspondingly precise gene expression map of C. elegans neurons wo...
  11. Isolation of Specific Neurons from C. elegans Larvae for Gene Expression Profiling.

    PLoS ONE 9(11):e112102 (2014) PMID 25372608 PMCID PMC4221280

    The simple and well-described structure of the C. elegans nervous system offers an unprecedented opportunity to identify the genetic programs that define the connectivity and function of individual neurons and their circuits. A correspondingly precise gene expression map of C. elegans neurons wo...
  12. Isolation of Specific Neurons from C. elegans Larvae for Gene Expression Profiling.

    PLoS ONE 9(11):e112102 (2014) PMID 25372608 PMCID PMC4221280

    The simple and well-described structure of the C. elegans nervous system offers an unprecedented opportunity to identify the genetic programs that define the connectivity and function of individual neurons and their circuits. A correspondingly precise gene expression map of C. elegans neurons wo...
  13. The million mutation project: a new approach to genetics in Caenorhabditis elegans.

    Genome Research 23(10):1749 (2013) PMID 23800452 PMCID PMC3787271

    We have created a library of 2007 mutagenized Caenorhabditis elegans strains, each sequenced to a target depth of 15-fold coverage, to provide the research community with mutant alleles for each of the worm's more than 20,000 genes. The library contains over 800,000 unique single nucleotide vari...
  14. The million mutation project: a new approach to genetics in Caenorhabditis elegans.

    Genome Research 23(10):1749 (2013) PMID 23800452 PMCID PMC3787271

    We have created a library of 2007 mutagenized Caenorhabditis elegans strains, each sequenced to a target depth of 15-fold coverage, to provide the research community with mutant alleles for each of the worm's more than 20,000 genes. The library contains over 800,000 unique single nucleotide vari...
  15. A high-fidelity cell lineage tracing method for obtaining systematic spatiotemporal gene expression patterns in Caenorhabditis elegans.

    G3: Genes, Genomes, Genetics 3(5):851 (2013) PMID 23550142 PMCID PMC3656732

    Advances in microscopy and fluorescent reporters have allowed us to detect the onset of gene expression on a cell-by-cell basis in a systemic fashion. This information, however, is often encoded in large repositories of images, and developing ways to extract this spatiotemporal expression data i...
  16. A high-fidelity cell lineage tracing method for obtaining systematic spatiotemporal gene expression patterns in Caenorhabditis elegans.

    G3: Genes, Genomes, Genetics 3(5):851 (2013) PMID 23550142 PMCID PMC3656732

    Advances in microscopy and fluorescent reporters have allowed us to detect the onset of gene expression on a cell-by-cell basis in a systemic fashion. This information, however, is often encoded in large repositories of images, and developing ways to extract this spatiotemporal expression data i...
  17. A high-fidelity cell lineage tracing method for obtaining systematic spatiotemporal gene expression patterns in Caenorhabditis elegans.

    G3: Genes, Genomes, Genetics 3(5):851 (2013) PMID 23550142 PMCID PMC3656732

    Advances in microscopy and fluorescent reporters have allowed us to detect the onset of gene expression on a cell-by-cell basis in a systemic fashion. This information, however, is often encoded in large repositories of images, and developing ways to extract this spatiotemporal expression data i...
  18. Genetic control of vulval development in Caenorhabditis briggsae.

    G3: Genes, Genomes, Genetics 2(12):1625 (2012) PMID 23275885 PMCID PMC3516484

    The nematode Caenorhabditis briggsae is an excellent model organism for the comparative analysis of gene function and developmental mechanisms. To study the evolutionary conservation and divergence of genetic pathways mediating vulva formation, we screened for mutations in C. briggsae that cause...
  19. Genetic control of vulval development in Caenorhabditis briggsae.

    G3: Genes, Genomes, Genetics 2(12):1625 (2012) PMID 23275885 PMCID PMC3516484

    The nematode Caenorhabditis briggsae is an excellent model organism for the comparative analysis of gene function and developmental mechanisms. To study the evolutionary conservation and divergence of genetic pathways mediating vulva formation, we screened for mutations in C. briggsae that cause...
  20. A Genome-Scale Resource for In Vivo Tag-Based Protein Function Exploration inC. elegans

    Cell 150(4):855 (2012)

    Understanding the in vivo dynamics of protein localization and their physical interactions is important for many problems in biology. To enable systematic protein function interrogation in a multicellular context, we built a genome-scale transgenic platform for in vivo expression of fl...