1. The addition of accessory enzymes enhances the hydrolytic performance of cellulase enzymes at high solid loadings.

    Bioresource Technology 186:149 (2015) PMID 25812819

    The pretreatment process used and the nature of the biomass feedstock will influence the role that accessory enzymes can play in synergistically interacting with cellulases to effectively deconstruct the substrate. The work reported here assessed the possible boosting effects of the xylanase and...
  2. How effective are traditional methods of compositional analysis in providing an accurate material balance for a range of softwood derived residues?

    Biotechnology for Biofuels 6(1):90 (2013) PMID 23800175 PMCID PMC3704954

    Forest residues represent an abundant and sustainable source of biomass which could be used as a biorefinery feedstock. Due to the heterogeneity of forest residues, such as hog fuel and bark, one of the expected challenges is to obtain an accurate material balance of these feedstocks. Current co...
  3. The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility

    Bioresource Technology 103(1):201 (2012) PMID 22047660

    Highlights ► The work compared two major influences of lignin during the enzymatic hydrolysis of steam pretreated Douglas-fir. ► The overall inhibitory effect of lignin is dependent on enzyme loading and cellulose accessibility. ► At low enzyme loading, lignin’s influence on ...
  4. Influence of steam pretreatment severity on post-treatments used to enhance the enzymatic hydrolysis of pretreated softwoods at low enzyme loadings.

    Biotechnology and Bioengineering 108(10):2300 (2011) PMID 21520024

    It is recognized that some form of post-treatment will usually be required if reasonable hydrolysis yields (>60%) of steam pretreated softwood are to be achieved when using low enzyme loadings (5 FPU/g cellulose). In the work reported here we modified/removed lignin from steam pretreated softwoo...
  5. Can the same steam pretreatment conditions be used for most softwoods to achieve good, enzymatic hydrolysis and sugar yields?

    Bioresource Technology 101(20):7827 (2010) PMID 20570139

    Wood chips from six different Douglas-fir trees and a representative Lodgepole pine were steam pretreated at a single pretreatment condition (200 °C 4% SO₂ 5 min) which had previously been shown to be effective for Spruce and Lodgepole pine chips. All of the softwood samples responded in a simil...
  6. Influence of xylan on the enzymatic hydrolysis of steam-pretreated corn stover and hybrid poplar.

    Biotechnology Progress 25(2):315 (2009) PMID 19266561

    The focus of this study was to alter the xylan content of corn stover and poplar using SO(2)-catalyzed steam pretreatment to determine the effect on subsequent hydrolysis by commercial cellulase preparations supplemented with or without xylanases. Steam pretreated solids with xylan contents rang...
  7. Rapid, microscale, acetyl bromide-based method for high-throughput determination of lignin content in Arabidopsis thaliana.

    Journal of Agricultural and Food Chemistry 56(16):6825 (2008) PMID 18666780

    The acetyl bromide method has been modified to enable the rapid microscale determination of lignin content in Arabidopsis with the goal of determining the genes that control lignin in plants. Modifications include reduction in sample size, use of a microball mill, adoption of a modified rapid me...
  8. The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis, part 1: a modified Simons' staining technique.

    Biotechnology Progress 24(5):1178 (2008) PMID 19194930

    To date, there is limited knowledge available regarding the key features of pretreated lignocellulosic substrates that promote the effective enzymatic hydrolysis of the cellulose component to glucose during bioconversion processes to produce ethanol. Fundamentally, cellulase enzymes require acce...