1. Comparative epigenomics in distantly related teleost species identifies conserved cis-regulatory nodes active during the vertebrate phylotypic period.

    Genome Research 24(7):1075 (2014) PMID 24709821 PMCID PMC4079964

    The complex relationship between ontogeny and phylogeny has been the subject of attention and controversy since von Baer's formulations in the 19th century. The classic concept that embryogenesis progresses from clade general features to species-specific characters has often been revisited. It h...
  2. Comparative epigenomics in distantly related teleost species identifies conserved cis-regulatory nodes active during the vertebrate phylotypic period.

    Genome Research 24(7):1075 (2014) PMID 24709821 PMCID PMC4079964

    The complex relationship between ontogeny and phylogeny has been the subject of attention and controversy since von Baer's formulations in the 19th century. The classic concept that embryogenesis progresses from clade general features to species-specific characters has often been revisited. It h...
  3. Comparative epigenomics in distantly related teleost species identifies conserved cis-regulatory nodes active during the vertebrate phylotypic period.

    Genome Research 24(7):1075 (2014) PMID 24709821 PMCID PMC4079964

    The complex relationship between ontogeny and phylogeny has been the subject of attention and controversy since von Baer's formulations in the 19th century. The classic concept that embryogenesis progresses from clade general features to species-specific characters has often been revisited. It h...
  4. Comparative epigenomics in distantly related teleost species identifies conserved cis-regulatory nodes active during the vertebrate phylotypic period.

    Genome Research 24(7):1075 (2014) PMID 24709821 PMCID PMC4079964

    The complex relationship between ontogeny and phylogeny has been the subject of attention and controversy since von Baer's formulations in the 19th century. The classic concept that embryogenesis progresses from clade general features to species-specific characters has often been revisited. It h...
  5. Comparative epigenomics in distantly related teleost species identifies conserved cis-regulatory nodes active during the vertebrate phylotypic period.

    Genome Research 24(7):1075 (2014) PMID 24709821 PMCID PMC4079964

    The complex relationship between ontogeny and phylogeny has been the subject of attention and controversy since von Baer's formulations in the 19th century. The classic concept that embryogenesis progresses from clade general features to species-specific characters has often been revisited. It h...
  6. Gene regulatory networks and transcriptional mechanisms that control myogenesis.

    Developmental Cell 28(3):225 (2014) PMID 24525185

    We discuss the upstream regulators of myogenesis that lead to the activation of myogenic determination genes and subsequent differentiation, focusing on the mouse model. Key upstream genes, such as Pax3 and Pax7, Six1 and Six4, or Pitx2, participate in gene regulatory networks at different sites...
  7. Gene regulatory networks and transcriptional mechanisms that control myogenesis.

    Developmental Cell 28(3):225 (2014) PMID 24525185

    We discuss the upstream regulators of myogenesis that lead to the activation of myogenic determination genes and subsequent differentiation, focusing on the mouse model. Key upstream genes, such as Pax3 and Pax7, Six1 and Six4, or Pitx2, participate in gene regulatory networks at different sites...
  8. Dial M(RF) for myogenesis.

    FEBS Journal 280(17):3980 (2013) PMID 23751110

    The transcriptional regulatory network that controls the determination and differentiation of skeletal muscle cells in the embryo has at its core the four myogenic regulatory factors (MRFs) Myf5, MyoD, Mrf4 and MyoG. These basic helix-loop-helix transcription factors act by binding, as obligate ...
  9. Dial M(RF) for myogenesis.

    FEBS Journal 280(17):3980 (2013) PMID 23751110

    The transcriptional regulatory network that controls the determination and differentiation of skeletal muscle cells in the embryo has at its core the four myogenic regulatory factors (MRFs) Myf5, MyoD, Mrf4 and MyoG. These basic helix-loop-helix transcription factors act by binding, as obligate ...
  10. Transcriptional dominance of Pax7 in adult myogenesis is due to high-affinity recognition of homeodomain motifs.

    Developmental Cell 22(6):1208 (2012) PMID 22609161 PMCID PMC3376216

    Pax3 and Pax7 regulate stem cell function in skeletal myogenesis. However, molecular insight into their distinct roles has remained elusive. Using gene expression data combined with genome-wide binding-site analysis, we show that both Pax3 and Pax7 bind identical DNA motifs and jointly activate ...
  11. Musculin and TCF21 coordinate the maintenance of myogenic regulatory factor expression levels during mouse craniofacial development.

    Development 139(5):958 (2012) PMID 22318627 PMCID PMC3274357

    The specification of the skeletal muscle lineage during craniofacial development is dependent on the activity of MYF5 and MYOD, two members of the myogenic regulatory factor family. In the absence of MYF5 or MYOD there is not an overt muscle phenotype, whereas in the double Myf5;MyoD knockout br...
  12. Musculin and TCF21 coordinate the maintenance of myogenic regulatory factor expression levels during mouse craniofacial development.

    Development 139(5):958 (2012) PMID 22318627 PMCID PMC3274357

    The specification of the skeletal muscle lineage during craniofacial development is dependent on the activity of MYF5 and MYOD, two members of the myogenic regulatory factor family. In the absence of MYF5 or MYOD there is not an overt muscle phenotype, whereas in the double Myf5;MyoD knockout br...
  13. Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments.

    Developmental Biology 355(2):372 (2011) PMID 21527258 PMCID PMC3123743

    The transcriptional regulation of the Mrf4/Myf5 locus depends on a multitude of enhancers that, in equilibria with transcription balancing sequences and the promoters, regulate the expression of the two genes throughout embryonic development and in the adult. Transcription in a particular set of...
  14. Regulation of gene expression in vertebrate skeletal muscle.

    Experimental Cell Research 316(18):3014 (2010) PMID 20633554

    During embryonic development the integration of numerous synergistic signalling pathways turns a single cell into a multicellular organism with specialized cell types and highly structured, organized tissues. To achieve this, cells must grow, proliferate, differentiate and die according to their...
  15. Interplay between DNA methylation and transcription factor availability: implications for developmental activation of the mouse Myogenin gene.

    Molecular and Cellular Biology 30(15):3805 (2010) PMID 20498275 PMCID PMC2916394

    During development, gene activation is stringently regulated to restrict expression only to the correct cell type and correct developmental stage. Here, we present mechanistic evidence that suggests DNA methylation contributes to this regulation by suppressing premature gene activation. Using th...
  16. Interplay between DNA methylation and transcription factor availability: implications for developmental activation of the mouse Myogenin gene.

    Molecular and Cellular Biology 30(15):3805 (2010) PMID 20498275 PMCID PMC2916394

    During development, gene activation is stringently regulated to restrict expression only to the correct cell type and correct developmental stage. Here, we present mechanistic evidence that suggests DNA methylation contributes to this regulation by suppressing premature gene activation. Using th...
  17. Global transcriptional regulation of the locus encoding the skeletal muscle determination genes Mrf4 and Myf5.

    Genes & Development 22(2):265 (2008) PMID 18198342 PMCID PMC2192759

    The linked Mrf4 and Myf5 genes encode two transcription factors essential for the determination and differentiation of skeletal muscle in the embryo. The locus is controlled by a multitude of interdigitated enhancers that activate gene expression at different times and in precisely defined proge...
  18. Global transcriptional regulation of the locus encoding the skeletal muscle determination genes Mrf4 and Myf5.

    Genes & Development 22(2):265 (2008) PMID 18198342 PMCID PMC2192759

    The linked Mrf4 and Myf5 genes encode two transcription factors essential for the determination and differentiation of skeletal muscle in the embryo. The locus is controlled by a multitude of interdigitated enhancers that activate gene expression at different times and in precisely defined proge...
  19. Expression of the Lingo/LERN gene family during mouse embryogenesis.

    Gene Expression Patterns 8(2):79 (2008) PMID 18297755

    We have analysed the expression during mouse development of the four member Lingo/LERN gene family which encodes type 1 transmembrane proteins containing 12 extracellular leucine rich repeats, an immunoglobulin C2 domain and a short intracellular tail. Each family member has a distinct pattern o...
  20. Mrf4 (myf6) is dynamically expressed in differentiated zebrafish skeletal muscle.

    Gene Expression Patterns 7(7):738 (2007) PMID 17638597 PMCID PMC3001336

    Mrf4 (Myf6) is a member of the basic helix-loop-helix (bHLH) myogenic regulatory transcription factor (MRF) family, which also contains Myod, Myf5 and myogenin. Mrf4 is implicated in commitment of amniote cells to skeletal myogenesis and is also abundantly expressed in many adult muscle fibres. ...