Mesenchymal stem cells in regenerative medicine: Focus on articular cartilage and intervertebral disc regeneration.
Methods 99:69 (2016)
Musculoskeletal disorders represent a major cause of disability and morbidity globally and result in enormous costs for health and social care systems. Development of cell-based therapies is rapidly proliferating in a number of disease areas, including musculoskeletal disorders. Novel biological...
Current Management Strategies in Breast Cancer by Targeting Key Altered Molecular Players.
Frontiers in Oncology 6:45 (2016)
Breast cancer is the second largest disease affecting women worldwide. It remains the most frequently reported and leading cause of death among women in both developed and developing countries. Tamoxifen and raloxifene are commonly used selective estrogen receptor modulators for treatment of bre...
Interleukin-33 exacerbates acute colitis via interleukin-4 in mice.
Immunology 140(1):70 (2013)
Interleukin-33 (IL-33) and its receptor ST2 are over-expressed in clinical colitis tissue. However, the significance of these observations is at present unknown. Significantly, we demonstrate here that IL33 and ST2 are the primary early genes induced in the inflamed colon of BALB/c mice followin...
Retraction: VAMP8 is essential in anaphylatoxin-induced degranulation, TNF-α secretion, peritonitis, and systemic inflammation.
Journal of Immunology 190(6):3007 (2013)
Retraction: Sphingosine kinase1 is pivotal for Fc"RI-mediated mast cell signaling and functional responses in vitro and in vivo.
Journal of Immunology 189(6):3262 (2012)
Retraction. The cytokine interleukin-33 mediates anaphylactic shock.
PNAS 109(34):13877 (2012)
IL-33 exacerbates autoantibody-induced arthritis.
Journal of Immunology 184(5):2620 (2010)
Rheumatoid arthritis pathogenesis comprises dysregulation in both innate and adaptive immunity. There is therefore intense interest in the factors that integrate these immunologic pathways in rheumatoid arthritis. In this paper, we report that IL-33, a novel member of the IL-1 family, can exacer...
Phospholipase D1 mediates TNFalpha-induced inflammation in a murine model of TNFalpha-induced peritonitis.
PLoS ONE 5(5):e10506 (2010)
Tumor Necrosis Factor alpha (TNFalpha) is a pleiotropic cytokine extensively studied for its role in the pathogenesis of a variety of disease conditions, including in inflammatory diseases. We have recently shown that, in vitro, that TNFalpha utilizes PLD1 to mediate the activation of NFkappaB a...
Resveratrol attenuates C5a-induced inflammatory responses in vitro and in vivo by inhibiting phospholipase D and sphingosine kinase activities.
FASEB Journal 23(8):2412 (2009)
The anti-inflammatory activity of the phytoalexin resveratrol (RSV) was evaluated in C5 anaphylatoxin (C5a)-stimulated primary neutrophils and in a mouse model of acute peritonitis. Pretreatment of human and mouse neutrophils with RSV significantly blocked oxidative burst, leukocyte migration, d...
VAMP8 is essential in anaphylatoxin-induced degranulation, TNF-alpha secretion, peritonitis, and systemic inflammation.
Journal of Immunology 183(2):1413 (2009)
VAMP8, a member of the soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) family of fusion proteins, initially characterized in endosomal and endosomal-lysosomal fusion, may also function in regulated exocytosis. VAMP8 physiological function in inflammation has...
Sphingosine kinase 1 is pivotal for Fc epsilon RI-mediated mast cell signaling and functional responses in vitro and in vivo.
Journal of Immunology 183(1):221 (2009)
Mast cell degranulation is pivotal to allergic diseases; investigating novel pathways triggering mast cell degranulation would undoubtedly have important therapeutic potential. FcepsilonRI-mediated degranulation has contradictorily been shown to require SphK1 or SphK2, depending on the reports. ...
The cytokine interleukin-33 mediates anaphylactic shock.
PNAS 106(24):9773 (2009)
Anaphylactic shock is characterized by elevated immunoglobulin-E (IgE) antibodies that signal via the high affinity Fc epsilon receptor (Fc epsilonRI) to release inflammatory mediators. Here we report that the novel cytokine interleukin-33 (IL-33) potently induces anaphylactic shock in mice and ...
Refining siRNA in vivo transfection: silencing SPHK1 reveals its key role in C5a-induced inflammation in vivo.
The International Journal of Biochemistry & Cel... 40(9):1817 (2008)
The transfection of siRNA in vivo is essential for the study of gene functions, target validation, and for gene therapy. However, the successful delivery of siRNA in whole organisms is still very difficult to achieve. A high-pressure delivery technique, called the "hydrodynamics" method, has bee...
Inhibition of Fc epsilon RI-mediated mast cell responses by ES-62, a product of parasitic filarial nematodes.
Nature Medicine 13(11):1375 (2007)
Atopic allergy is characterized by an increase in IgE antibodies that signal through the high-affinity Fcepsilon receptor (FcepsilonRI) to induce the release of inflammatory mediators from mast cells. For unknown reasons, the prevalence of allergic diseases has recently increased steeply in the ...
Protein i: interference at protein level by intrabodies.
Frontiers in Bioscience 12:1344 (2007)
Immunoglobulin molecules have long been used in biomedical research as in vitro tools for identification, purification and functional manipulation of target proteins. The specificity and diversity of immunoglobulins can be exploited to target a wide range of intracellular proteins by expressing ...
Short interfering RNA (siRNA) as a novel therapeutic.
Clinical and Experimental Pharmacology and Phys... 33(5-6):504 (2006)
1. RNA interference (RNAi) is a robust method of post-transcriptional silencing of genes using double-stranded RNA (dsRNA) with sequence homology driven specificity. The dsRNA can be between 21 and 23 nucleotides long: this is converted to small interfering RNA (siRNA), which then mediates gene ...