1. The polar auxin transport inhibitor TIBA inhibits endoreduplication in dark grown spinach hypocotyls.

    Plant Science 225:45 (2014) PMID 25017158

    We addressed the question of whether an additional round of endoreduplication in dark-grown hypocotyls is a common feature in dicotyledonous plants having endopolyploid tissues. Ploidy distributions of hypocotyl tissues derived from in vitro-grown spinach (Spinacia oleracea L. cv. Atlas) seedlin...
  2. The polar auxin transport inhibitor TIBA inhibits endoreduplication in dark grown spinach hypocotyls.

    Plant Science 225:45 (2014) PMID 25017158

    We addressed the question of whether an additional round of endoreduplication in dark-grown hypocotyls is a common feature in dicotyledonous plants having endopolyploid tissues. Ploidy distributions of hypocotyl tissues derived from in vitro-grown spinach (Spinacia oleracea L. cv. Atlas) seedlin...
  3. The polar auxin transport inhibitor TIBA inhibits endoreduplication in dark grown spinach hypocotyls

    Plant Science 225:45 (2014)

    • An additional round of endoreduplication was found in dark-grown spinach hypocotyls. • Inhibition of the endoreduplication depends on the light intensity. ...
  4. The polar auxin transport inhibitor TIBA inhibits endoreduplication in dark grown spinach hypocotyls.

    Plant Science 225:45 (2014) PMID 25017158

    We addressed the question of whether an additional round of endoreduplication in dark-grown hypocotyls is a common feature in dicotyledonous plants having endopolyploid tissues. Ploidy distributions of hypocotyl tissues derived from in vitro-grown spinach (Spinacia oleracea L. cv. Atlas) seedlin...
  5. [Conservation and divergence of cytoplasmic splicing in the organisms].

    Seikagaku. The Journal of Japanese Biochemical ... 85(12):1095 (2013) PMID 24505943

  6. Defects in IRE1 enhance cell death and fail to degrade mRNAs encoding secretory pathway proteins in the Arabidopsis unfolded protein response.

    PNAS 110(14):5713 (2013) PMID 23509268 PMCID PMC3619347

    The unfolded protein response (UPR) is a cellular response highly conserved in eukaryotes to obviate accumulation of misfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring enzyme 1 (IRE1) catalyzes the cytoplasmic splicing of mRNA encoding bZIP transcription factors to activate...
  7. An attempt to detect siRNA-mediated genomic DNA modification by artificially induced mismatch siRNA in Arabidopsis.

    PLoS ONE 8(11):e81326 (2013) PMID 24278423 PMCID PMC3837478

    Although tremendous progress has been made in recent years in identifying molecular mechanisms of small interfering RNA (siRNA) functions in higher plants, the possibility of direct interaction between genomic DNA and siRNA remains an enigma. Such an interaction was proposed in the 'RNA cache' h...
  8. An attempt to detect siRNA-mediated genomic DNA modification by artificially induced mismatch siRNA in Arabidopsis.

    PLoS ONE 8(11):e81326 (2013) PMID 24278423 PMCID PMC3837478

    Although tremendous progress has been made in recent years in identifying molecular mechanisms of small interfering RNA (siRNA) functions in higher plants, the possibility of direct interaction between genomic DNA and siRNA remains an enigma. Such an interaction was proposed in the 'RNA cache' h...
  9. Plant transducers of the endoplasmic reticulum unfolded protein response.

    Trends in Plant Science 17(12):720 (2012) PMID 22796463

    The unfolded protein response (UPR) activates a set of genes to overcome accumulation of unfolded proteins in the endoplasmic reticulum (ER), a condition termed ER stress, and constitutes an essential part of ER protein quality control that ensures efficient maturation of secretory and membrane ...
  10. Development of a series of gateway binary vectors possessing a tunicamycin resistance gene as a marker for the transformation of Arabidopsis thaliana.

    Bioscience, Biotechnology, and Biochemistry 75(4):804 (2011) PMID 21512216

    We made two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing a UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene driven by the nopaline synthase promoter (Pnos) as a tunicamycin resistance marker for the transformation of Arabidopsis thalia...
  11. Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor.

    Scientific reports 1:29 (2011) PMID 22355548 PMCID PMC3216516

    IRE1 plays an essential role in the endoplasmic reticulum (ER) stress response in yeast and mammals. We found that a double mutant of Arabidopsis IRE1A and IRE1B (ire1a/ire1b) is more sensitive to the ER stress inducer tunicamycin than the wild-type. Transcriptome analysis revealed that genes wh...
  12. Development of a series of gateway binary vectors possessing a tunicamycin resistance gene as a marker for the transformation of Arabidopsis thaliana.

    Bioscience, Biotechnology, and Biochemistry 75(4):804 (2011) PMID 21512216

    We made two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing a UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene driven by the nopaline synthase promoter (Pnos) as a tunicamycin resistance marker for the transformation of Arabidopsis thalia...
  13. Characterization of a plant-specific gene induced by endoplasmic reticulum stress in Arabidopsis thaliana.

    Bioscience, Biotechnology, and Biochemistry 74(10):2087 (2010) PMID 20944397

    We have conducted transcriptome analysis and have identified a number of genes that are upregulated by treatment with the endoplasmic reticulum (ER) stress inducer tunicamycin. Here we focused on one particular gene of unknown function. This gene, designated tunicamycin induced 1 (TIN1), encodes...
  14. Characterization of a plant-specific gene induced by endoplasmic reticulum stress in Arabidopsis thaliana.

    Bioscience, Biotechnology, and Biochemistry 74(10):2087 (2010) PMID 20944397

    We have conducted transcriptome analysis and have identified a number of genes that are upregulated by treatment with the endoplasmic reticulum (ER) stress inducer tunicamycin. Here we focused on one particular gene of unknown function. This gene, designated tunicamycin induced 1 (TIN1), encodes...
  15. The Arabidopsis membrane-bound transcription factor AtbZIP60 is a novel plant-specific endoplasmic reticulum stress transducer.

    Plant Signaling & Behavior 4(6):514 (2009) PMID 19816135 PMCID PMC2688297

  16. The Arabidopsis membrane-bound transcription factor AtbZIP60 is a novel plant-specific endoplasmic reticulum stress transducer.

    Plant Signaling & Behavior 4(6):514 (2009) PMID 19816135 PMCID PMC2688297

  17. Arabidopsis bZIP60 is a proteolysis-activated transcription factor involved in the endoplasmic reticulum stress response.

    Plant Cell 20(11):3107 (2008) PMID 19017746 PMCID PMC2613661

    Proteins synthesized in the endoplasmic reticulum (ER) of eukaryotic cells must be folded correctly before translocation out of the ER. Disruption of protein folding results in the induction of genes for ER-resident chaperones, for example, BiP. This phenomenon is known as the ER stress response...
  18. NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum.

    Journal of Plant Research 121(6):603 (2008) PMID 18758894

    A spermine-based signal transduction pathway plays a defensive role against incompatible pathogens. We identified a novel spermine-responsive cDNA from Nicotiana tabacum that encodes a basic region/leucine zipper protein with a putative transmembrane domain. Identity to Arabidopsis thaliana AtbZ...
  19. Construction of a binary vector for transformation of Arabidopsis thaliana with a new selection marker.

    Bioscience, Biotechnology, and Biochemistry 72(11):3041 (2008) PMID 18997421

    A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation...
  20. Construction of a binary vector for transformation of Arabidopsis thaliana with a new selection marker.

    Bioscience, Biotechnology, and Biochemistry 72(11):3041 (2008) PMID 18997421

    A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation...