1. Multidimensional High-Resolution Magic Angle Spinning and Solution-State NMR Characterization of (13)C-labeled Plant Metabolites and Lignocellulose.

    Scientific reports 5:11848 (2015) PMID 26143886 PMCID PMC4491710

    Lignocellulose, which includes mainly cellulose, hemicellulose, and lignin, is a potential resource for the production of chemicals and for other applications. For effective production of materials derived from biomass, it is important to characterize the metabolites and polymeric components of ...
  2. Metabolic flux analysis of genetically engineered Saccharomyces cerevisiae that produces lactate under micro-aerobic conditions.

    Bioprocess and Biosystems Engineering 36(9):1261 (2013) PMID 23223911

    In the present study, to elucidate mechanisms of growth suppression in YIBO-pdc1/5Δ, we performed carbon metabolic flux analysis under micro-aerobic conditions. Our results indicate that growth suppression of YIBO-pdc1/5Δ is caused by decreased flux to the pentose phosphate pathway, which suppli...
  3. Metabolic flux analysis of genetically engineered Saccharomyces cerevisiae that produces lactate under micro-aerobic conditions.

    Bioprocess and Biosystems Engineering 36(9):1261 (2013) PMID 23223911

    In the present study, to elucidate mechanisms of growth suppression in YIBO-pdc1/5Δ, we performed carbon metabolic flux analysis under micro-aerobic conditions. Our results indicate that growth suppression of YIBO-pdc1/5Δ is caused by decreased flux to the pentose phosphate pathway, which suppli...
  4. Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae.

    Journal of Bioscience and Bioengineering 115(5):467 (2013) PMID 23290995

    To create strains that have high productivity of lactic acid without neutralization, a genome-wide screening for strains showing hyper-resistance to 6% l-lactic acid (pH 2.6) was performed using the gene deletion collection of Saccharomyces cerevisiae. We identified 94 genes whose disruption led...
  5. Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass.

    Bioresource Technology 135:103 (2013) PMID 22850175

    The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80 °C or 100 °C. Short [Hpy][Cl] pretreatments, <30 min, at lower temperature accelerate subsequent enzymatic saccharification of A...
  6. Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass

    Bioresource Technology 135:103 (2013)

    The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80°C or 100°C. Short [Hpy][Cl] pretreatments, <30min, at lower temperature accelerate subsequent enzymatic saccharificati...
  7. Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity inSaccharomyces cerevisiae

    Journal of Bioscience and Bioengineering 115(5):467 (2013)

    To create strains that have high productivity of lactic acid without neutralization, a genome-wide screening for strains showing hyper-resistance to 6% l-lactic acid (pH 2.6) was performed using the gene deletion collection of Saccharomyces cerevisiae. We identified 94 genes whose disr...
  8. Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae.

    Journal of Bioscience and Bioengineering 115(5):467 (2013) PMID 23290995

    To create strains that have high productivity of lactic acid without neutralization, a genome-wide screening for strains showing hyper-resistance to 6% l-lactic acid (pH 2.6) was performed using the gene deletion collection of Saccharomyces cerevisiae. We identified 94 genes whose disruption led...
  9. Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass

    Bioresource Technology 135:103 (2013) PMID 22850175

    The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80°C or 100°C. Short [Hpy][Cl] pretreatments, <30min, at lower temperature accelerate subsequent enzymatic saccharificati...
  10. Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter

    Journal of Bioscience and Bioengineering 115(2):193 (2013) PMID 23021912

    A simple fed-batch system for cultivating genetically engineered yeast generating lactate under the regulation of the PDC1 promoter was established. Traditional strategies that avoid occurrence of Crabtree effect, such as respiratory quotient (RQ) control or ethanol control, are not ap...
  11. Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter.

    Journal of Bioscience and Bioengineering 115(2):193 (2013) PMID 23021912

    A simple fed-batch system for cultivating genetically engineered yeast generating lactate under the regulation of the PDC1 promoter was established. Traditional strategies that avoid occurrence of Crabtree effect, such as respiratory quotient (RQ) control or ethanol control, are not applicable t...
  12. Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter

    Journal of Bioscience and Bioengineering 115(2):193 (2013)

    A simple fed-batch system for cultivating genetically engineered yeast generating lactate under the regulation of the PDC1 promoter was established. Traditional strategies that avoid occurrence of Crabtree effect, such as respiratory quotient (RQ) control or ethanol control, are not ap...
  13. Amino acid residues important for CMP-sialic acid recognition by the CMP-sialic acid transporter: analysis of the substrate specificity of UDP-galactose/CMP-sialic acid transporter chimeras.

    Glycobiology 22(12):1731 (2012) PMID 22833315

    In our previous studies, we demonstrated that chimeric molecules of the CMP-sialic acid (CMP-Sia) transporter (CST) and the UDP-galactose (Gal) transporter (UGT) in which the seventh transmembrane helix-containing segment was derived from the CST could transport both CMP-Sia and UDP-Gal and that...
  14. Amino acid residues important for CMP-sialic acid recognition by the CMP-sialic acid transporter: analysis of the substrate specificity of UDP-galactose/CMP-sialic acid transporter chimeras.

    Glycobiology 22(12):1731 (2012) PMID 22833315

    In our previous studies, we demonstrated that chimeric molecules of the CMP-sialic acid (CMP-Sia) transporter (CST) and the UDP-galactose (Gal) transporter (UGT) in which the seventh transmembrane helix-containing segment was derived from the CST could transport both CMP-Sia and UDP-Gal and that...
  15. Exploring the conformational space of amorphous cellulose using NMR chemical shifts

    Carbohydrate Polymers 90(3):1197 (2012) PMID 22939331

    Highlights ► 13C-labeled cellulose synthesized by Acetobacter xylinum for NMR experiments. ► Experimental and calculated chemical shifts (CS) obtained for amorphous cellulose. ► Experimental CS for amorphous cellulose were assigned. ► Molecular dynamics simulations and quantu...
  16. Exploring the conformational space of amorphous cellulose using NMR chemical shifts

    Carbohydrate Polymers 90(3):1197 (2012) PMID 22939331

    Highlights ► 13C-labeled cellulose synthesized by Acetobacter xylinum for NMR experiments. ► Experimental and calculated chemical shifts (CS) obtained for amorphous cellulose. ► Experimental CS for amorphous cellulose were assigned. ► Molecular dynamics simulations and quantu...
  17. Exploring the conformational space of amorphous cellulose using NMR chemical shifts

    Carbohydrate Polymers 90(3):1197 (2012)

    Highlights ► 13C-labeled cellulose synthesized by Acetobacter xylinum for NMR experiments. ► Experimental and calculated chemical shifts (CS) obtained for amorphous cellulose. ► Experimental CS for amorphous cellulose were assigned. ► Molecular dynamics simulations and quantu...
  18. Exploring the conformational space of amorphous cellulose using NMR chemical shifts.

    Carbohydrate polymers 90(3):1197 (2012) PMID 22939331

    (13)C-labeled amorphous cellulose and (13)C NMR chemical shifts by 2D (13)C-(13)C correlation spectroscopy were obtained in the regenerated solid-state from ionic liquids. On the basis of the assigned chemical shifts, combined with information from molecular dynamics and quantum chemistry comput...
  19. Investigation of utilization of the algal biomass residue after oil extraction to lower the total production cost of biodiesel

    Journal of Bioscience and Bioengineering 114(3):330 (2012)

    In this study, component analysis of a novel biodiesel-producing alga, Pseudochoricystis ellipsoidea, was performed. The component analysis results indicated that proteins and amino acids are abundant in P. ellipsoidea while the sugar content is relatively low. Rather than its use as a...
  20. Investigation of utilization of the algal biomass residue after oil extraction to lower the total production cost of biodiesel.

    Journal of Bioscience and Bioengineering 114(3):330 (2012) PMID 22627052

    In this study, component analysis of a novel biodiesel-producing alga, Pseudochoricystis ellipsoidea, was performed. The component analysis results indicated that proteins and amino acids are abundant in P. ellipsoidea while the sugar content is relatively low. Rather than its use as a carbon so...