1. C-terminal residues regulate localization and function of the antiapoptotic protein Bfl-1.

    Journal of Biological Chemistry 284(44):30257 (2009) PMID 19759007 PMCID PMC2781581

    Unlike other antiapoptotic members of the Bcl-2 family, Bfl-1 does not contain a well defined C-terminal transmembrane domain, and whether the C-terminal tail of Bfl-1 functions as a membrane anchor is not yet clearly established. The molecular modeling study of the full-length Bfl-1 performed w...
  2. Structure-activity relationships of Bak derived peptides: affinity and specificity modulations by amino acid replacement.

    European Journal of Medicinal Chemistry 43(5):966 (2008) PMID 17692431

    To study the structure-activity relationships (SAR) and the binding activity of pro-apoptotic Bak BH3 domain, we synthesised several 16mer peptide analogues corresponding to the region (72)-GQVGRQLAIIGDDINR-(87). Using different amino acids varying in length, steric and electronic properties, we...
  3. Structure–activity relationships of Bak derived peptides: Affinity and specificity modulations by amino acid replacement

    European Journal of Medicinal Chemistry 43(5):966 (2008)

    To study the structure–activity relationships (SAR) and the binding activity of pro-apoptotic Bak BH3 domain, we synthesised several 16mer peptide analogues corresponding to the region 72-GQVGRQLAIIGDDINR- 87. Using different amino acids varying in length, steric and electronic...
  4. A simple theoretical model for fluorescence polarization binding assay development.

    Journal of Biomolecular Screening 11(8):949 (2006) PMID 17092915

    Fluorescence polarization is a screening technology that is radioactivity free, homogeneous, and ratiometric. The signal measured with this technology is a weighted value of free and bound ligand. As a consequence, saturation curves are accessible only after calculation of the corresponding conc...
  5. The small organic compound HA14-1 prevents Bcl-2 interaction with Bax to sensitize malignant glioma cells to induction of cell death.

    Cancer Research 66(5):2757 (2006) PMID 16510597

    A functional imbalance between proapoptotic Bax and antiapoptotic Bcl-2 is likely to participate in the resistance of cancer cells to therapy. We show here that ethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (HA14-1), a small organic compound recently proposed to...