1. A Massive Expansion of Effector Genes Underlies Gall-Formation in the Wheat Pest Mayetiola destructor.

    Current Biology 25(5):613 (2015) PMID 25660540

    Gall-forming arthropods are highly specialized herbivores that, in combination with their hosts, produce extended phenotypes with unique morphologies [1]. Many are economically important, and others have improved our understanding of ecology and adaptive radiation [2]. However, the mechanisms th...
  2. Unbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat.

    Scientific reports 5:8092 (2015) PMID 25627558 PMCID PMC4308708

    Glutathione, γ-glutamylcysteinylglycine, exists abundantly in nearly all organisms. Glutathione participates in various physiological processes involved in redox reactions by serving as an electron donor/acceptor. We found that the abundance of total glutathione increased up to 60% in resistant ...
  3. Unbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat.

    Scientific reports 5:8092 (2015) PMID 25627558

    Glutathione, γ-glutamylcysteinylglycine, exists abundantly in nearly all organisms. Glutathione participates in various physiological processes involved in redox reactions by serving as an electron donor/acceptor. We found that the abundance of total glutathione increased up to 60% in resistant ...
  4. A Massive Expansion of Effector Genes Underlies Gall-Formation in the Wheat Pest Mayetiola destructor

    Current Biology (2014)

    • The plant galling Mayetiola destructor genome is replete with effector genes • The SSGP-71 effector gene family is the largest known arthropod gene family. ...
  5. Molecular markers for species identification of Hessian fly males caught on sticky pheromone traps.

    Journal of Economic Entomology 107(3):1110 (2014) PMID 25026671

    Pheromone traps have been widely used to monitor insect population activity. However, sticky pheromone traps for the Hessian fly (Mayetiola destructor), one of the most destructive pests of wheat, have been used only in recent years. Hessian fly male adults are small and fragile, and preserving ...
  6. Impact of temperatures on Hessian fly (Diptera: Cecidomyiidae) resistance in selected wheat cultivars (Poales: Poaceae) in the Great Plains region.

    Journal of Economic Entomology 107(3):1266 (2014) PMID 25026692

    Changes in temperature can result in fundamental changes in plant physiology. This study investigated the impact of different temperatures from 14 to 26 degrees C on the resistance or susceptibility to the Hessian fly, Mayetiola destructor (Say), of selected wheat cultivars that are either curre...
  7. Virulence and biotype analyses of Hessian fly (Diptera: Cecidomyiidae) populations from Texas, Louisiana, and Oklahoma.

    Journal of Economic Entomology 107(1):417 (2014) PMID 24665728

    Hessian fly, Mayetiola destructor (Say, 1817), is a major pest of wheat, and is controlled mainly through deploying fly-resistant wheat cultivars. The challenge for the plant resistance approach is that virulence of Hessian fly populations in the field is dynamic, and wheat cultivars may lose re...
  8. Transient heat stress compromises the resistance of wheat (Poales: Poaceae) seedlings to Hessian fly (Diptera: Cecidomyiidae) infestation.

    Journal of Economic Entomology 107(1):389 (2014) PMID 24665724

    Heat stress exerts a profound impact on the resistance of plants to parasites. In this research, we investigated the impact of an acute transient heat stress on the resistance of the wheat line 'Molly,' which contains the R gene H13, to an avirulent Hessian fly (Mayetiola destructor (Say)) popul...
  9. Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).

    PLoS ONE 9(6):e100958 (2014) PMID 24964065 PMCID PMC4071006

    Highly specialized obligate plant-parasites exist within several groups of arthropods (insects and mites). Many of these are important pests, but the molecular basis of their parasitism and its evolution are poorly understood. One hypothesis is that plant parasitic arthropods use effector protei...
  10. Pyrosequencing reveals the predominance of pseudomonadaceae in gut microbiome of a gall midge.

    Pathogens 3(2):459 (2014) PMID 25437809

    Gut microbes are known to play various roles in insects such as digestion of inaccessible nutrients, synthesis of deficient amino acids, and interaction with ecological environments, including host plants. Here, we analyzed the gut microbiome in Hessian fly, a serious pest of wheat. A total of 3...
  11. Pyrosequencing reveals the predominance of pseudomonadaceae in gut microbiome of a gall midge.

    Pathogens 3(2):459 (2014) PMID 25437809 PMCID PMC4243456

    Gut microbes are known to play various roles in insects such as digestion of inaccessible nutrients, synthesis of deficient amino acids, and interaction with ecological environments, including host plants. Here, we analyzed the gut microbiome in Hessian fly, a serious pest of wheat. A total of 3...
  12. Serine and cysteine protease-like genes in the genome of a gall midge and their interactions with host plant genotypes.

    Insect Biochemistry and Molecular Biology 43(8):701 (2013) PMID 23727407

    Proteases play important roles in a wide range of physiological processes in organisms. For plant-feeding insects, digestive proteases are targets for engineering protease inhibitors for pest control. In this study, we identified 105 putative serine- and cysteine-protease genes from the genome o...
  13. Serine and cysteine protease-like genes in the genome of a gall midge and their interactions with host plant genotypes

    Insect Biochemistry and Molecular Biology 43(8):701 (2013)

    Proteases play important roles in a wide range of physiological processes in organisms. For plant-feeding insects, digestive proteases are targets for engineering protease inhibitors for pest control. In this study, we identified 105 putative serine- and cysteine-protease genes from th...
  14. Genetic association of OPR genes with resistance to Hessian fly in hexaploid wheat.

    BMC Genomics 14:369 (2013) PMID 23724909 PMCID PMC3674912

    Hessian fly (Mayetiola destructor) is one of the most destructive pests of wheat. The genes encoding 12-oxo-phytodienoic acid reductase (OPR) and lipoxygenase (LOX) play critical roles in insect resistance pathways in higher plants, but little is known about genes controlling resistance to Hessi...
  15. Deep sequencing and genome-wide analysis reveals the expansion of MicroRNA genes in the gall midge Mayetiola destructor.

    BMC Genomics 14:187 (2013) PMID 23496979 PMCID PMC3608969

    MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in regulating post transcriptional gene expression. Gall midges encompass a large group of insects that are of economic importance and also possess fascinating biological traits. The gall midge Mayetiola destructor, commonly k...
  16. Wheat Mds-1 encodes a heat-shock protein and governs susceptibility towards the Hessian fly gall midge.

    Nature Communications 4:2070 (2013) PMID 23792912

    Gall midges induce formation of host nutritive cells and alter plant metabolism to utilize host resources. Here we show that the gene Mayetiola destructor susceptibility-1 on wheat chromosome 3AS encodes a small heat-shock protein and is a major susceptibility gene for infestation of wheat by th...
  17. Mobilization of lipids and fortification of cell wall and cuticle are important in host defense against Hessian fly.

    BMC Genomics 14:423 (2013) PMID 23800119 PMCID PMC3701548

    Wheat - Hessian fly interaction follows a typical gene-for-gene model. Hessian fly larvae die in wheat plants carrying an effective resistance gene, or thrive in susceptible plants that carry no effective resistance gene. Gene sets affected by Hessian fly attack in resistant plants were found to...
  18. TSG: a new algorithm for binary and multi-class cancer classification and informative genes selection.

    BMC Medical Genomics 6 Suppl 1:S3 (2013) PMID 23445528 PMCID PMC3552704

    One of the challenges in classification of cancer tissue samples based on gene expression data is to establish an effective method that can select a parsimonious set of informative genes. The Top Scoring Pair (TSP), k-Top Scoring Pairs (k-TSP), Support Vector Machines (SVM), and prediction analy...
  19. Rapid mobilization of membrane lipids in wheat leaf sheaths during incompatible interactions with Hessian fly.

    Molecular Plant-Microbe Interactions 25(7):920 (2012) PMID 22668001 PMCID PMC3586561

    Hessian fly (HF) is a biotrophic insect that interacts with wheat on a gene-for-gene basis. We profiled changes in membrane lipids in two isogenic wheat lines: a susceptible line and its backcrossed offspring containing the resistance gene H13. Our results revealed a 32 to 45% reduction in total...
  20. Gall midges (Hessian flies) as plant pathogens.

    Phytopathology 50:339 (2012) PMID 22656645

    Gall midges constitute an important group of plant-parasitic insects. The Hessian fly (HF; Mayetiola destructor), the most investigated gall midge, was the first insect hypothesized to have a gene-for-gene interaction with its host plant, wheat (Triticum spp.). Recent investigations support that...