The transcription factor ATF2 promotes melanoma metastasis by suppressing protein fucosylation.
Science Signaling 8(406):ra124 (2015)
Melanoma is one of the most lethal skin cancers worldwide, primarily because of its propensity to metastasize. Thus, the elucidation of mechanisms that govern metastatic propensity is urgently needed. We found that protein kinase Cε (PKCε)-mediated activation of activating transcription factor 2...
Overexpression of zinc-finger protein 777 (ZNF777) inhibits proliferation at low cell density through down-regulation of FAM129A.
Journal of Cellular Biochemistry 116(6):954 (2015)
Krüppel-associated box-containing zinc finger proteins (KRAB-ZFPs) regulate a wide range of cellular processes. KRAB-ZFPs have a KRAB domain, which binds to transcriptional corepressors, and a zinc finger domain, which binds to DNA to activate or repress gene transcription. Here, we characterize...
Determination of carbohydrate structure recognized by prostate-specific F77 monoclonal antibody through expression analysis of glycosyltransferase genes.
Journal of Biological Chemistry 289(23):16478 (2014)
This study reports the determination of the carbohydrate epitope of monoclonal antibody F77 previously raised against human prostate cancer PC-3 cells (Zhang, G., Zhang, H., Wang, Q., Lal, P., Carroll, A. M., de la Llera-Moya, M., Xu, X., and Greene, M. I. (2010) Proc. Natl. Acad. Sci. U. S. A. ...
Carbohydrate sequence of the prostate cancer-associated antigen F77 assigned by a mucin O-glycome designer array.
Journal of Biological Chemistry 289(23):16462 (2014)
Monoclonal antibody F77 was previously raised against human prostate cancer cells and has been shown to recognize a carbohydrate antigen, but the carbohydrate sequence of the antigen was elusive. Here, we make multifaceted approaches to characterize F77 antigen, including binding analyses with t...
Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans.
FEBS Letters 587(19):3195 (2013)
Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acet...
2108 BLOOD GROUP ANTIGEN-TARGETING PEPTIDE SUPPRESSES ANTI-BLOOD GROUP ANTIBODY BINDING TO ANTIGEN IN RENAL GLOMERULAR CAPILLARIES AFTER ABO-INCOMPATIBLE BLOOD REPERFUSION
The Journal of Urology 189(4):e864 (2013)
In vivo regulation of steroid hormones by the Chst10 sulfotransferase in mouse.
Journal of Biological Chemistry 288(7):5007 (2013)
Chst10 adds sulfate to glucuronic acid to form a carbohydrate antigen, HNK-1, in glycoproteins and glycolipids. To determine the role of Chst10 in vivo, we generated systemic Chst10-deficient mutant mice. Although Chst10(-/-) mice were born and grew to adulthood with no gross defects, they were ...
Blood group antigen-targeting peptide suppresses anti-blood group antibody binding to antigen in renal glomerular capillaries after ABO-incompatible blood reperfusion.
Transplantation 95(3):418 (2013)
Antibody-mediated rejection after ABO-incompatible kidney transplantation (ABO-I KTx) is a major barrier to transplantation success. The advent of immunosuppressive therapy has markedly improved graft survival in ABO-I KTx. However, compared with normal KTx, clinical conditions during ABO-I KTx ...
Priming mass spectrometry-based sulfoglycomic mapping for identification of terminal sulfated lacdiNAc glycotope.
Glycoconjugate Journal 30(2):183 (2013)
In an effort to prime our mass spectrometry (MS)-based sulfoglycomic mapping platform technology for facile identification of sulfated lacdiNAc (GalNAcβ1-4GlcNAcβ1-), we have re-examined the N-glycans of bovine thyroid stimulating hormone. We showed that MALDI-MS mapping of permethylated glycans...
Tumor targeting by a carbohydrate ligand-mimicking peptide.
Methods in Molecular Biology 1022:369 (2013)
Annexin A1 (Anxa1) is a highly specific surface marker of tumor vasculature. We used peptide-displaying phage technology to identify a carbohydrate ligand-mimicking 7-mer peptide, IFLLWQR (IF7), which can target Anxa1 in tumor vasculature. Here, we describe the binding activity of carbohydrate t...
Cell adhesion molecules in human embryo implantation.
Sheng li xue bao : [Acta physiologica Sinica] 64(3):247 (2012)
The process of human embryo implantation is mediated not only by evolutionarily conserved mechanisms, but also by a mechanism unique to humans. Evidence suggests that the cell adhesion molecules, L-selectin and trophinin, play a unique role in human embryo implantation. Here, we describe the dua...
Peptide-displaying phage technology in glycobiology.
Glycobiology 22(3):318 (2012)
Phage display technology is an emerging drug discovery tool. Using that approach, short peptides that mimic part of a carbohydrate's conformation are selected by screening a peptide-displaying phage library with anti-carbohydrate antibodies. Chemically synthesized peptides with an identified seq...
Essential role of gastric gland mucin in preventing gastric cancer in mice.
Journal of Clinical Investigation 122(3):923 (2012)
Gastric gland mucin secreted from the lower portion of the gastric mucosa contains unique O-linked oligosaccharides (O-glycans) having terminal α1,4-linked N-acetylglucosamine residues (αGlcNAc). Previously, we identified human α1,4-N-acetylglucosaminyltransferase (α4GnT), which is responsible f...
Identification of mono- and disulfated N-acetyl-lactosaminyl Oligosaccharide structures as epitopes specifically recognized by humanized monoclonal antibody HMOCC-1 raised against ovarian cancer.
Journal of Biological Chemistry 287(9):6592 (2012)
A humanized monoclonal antibody raised against human ovarian cancer RMG-I cells and designated as HMOCC-1 (Suzuki, N., Aoki, D., Tamada, Y., Susumu, N., Orikawa, K., Tsukazaki, K., Sakayori, M., Suzuki, A., Fukuchi, T., Mukai, M., Kojima-Aikawa, K., Ishida, I., and Nozawa, S. (2004) Gynecol. Onc...
Enhancement of mouse sperm motility by trophinin-binding peptide.
Reproductive Biology and Endocrinology 10:101 (2012)
Trophinin is an intrinsic membrane protein that forms a complex in the cytoplasm with bystin and tastin, linking it microtubule-associated motor dynein (ATPase) in some cell types. Previously, we found that human sperm tails contain trophinin, bystin and tastin proteins, and that trophinin-bindi...
Trophinin in cell adhesion and signal transduction.
Frontiers in bioscience (elite edition) 4:342 (2012)
The process of human embryo implantation is mediated not only by evolutionarily conserved mechanisms but by activities unique to humans. Among the latter, evidence suggests that the cell adhesion molecule trophinin plays a unique role in human embryo implantation. Here, we describe characteristi...
Identification of endothelial cell surface carbohydrate-binding receptors by carbohydrate ligand mimicry peptides.
Advances in Experimental Medicine and Biology 749:57 (2012)
Carbohydrate mimetic peptides as research reagent and therapeutic.
Biological & Pharmaceutical Bulletin 35(10):1626 (2012)
Although numerous carbohydrates play significant roles in mammalian cells, development of carbohydrate-based reagents and therapeutics are hampered by the technical difficulty of chemically synthesizing complex carbohydrate structures. Use of carbohydrate mimetic peptides circumvents this diffic...
Targeted drug delivery to tumor vasculature by a carbohydrate mimetic peptide.
PNAS 108(49):19587 (2011)
Although numerous carbohydrates play significant roles in mammalian cells, carbohydrate-based drug discovery has not been explored due to the technical difficulty of chemically synthesizing complex carbohydrate structures. Previously, we identified a series of carbohydrate mimetic peptides and f...
Trophinin-mediated cell adhesion induces apoptosis of human endometrial epithelial cells through PKC-δ.
Cell Cycle 10(1):135 (2011)
Trophinin is an intrinsic membrane protein expressed in trophectoderm cells of embryos and in uterine epithelial cells. Trophinin potentially mediates apical cell adhesion at human embryo implantation sites through trophinin-trophinin binding in these two cell types. Trophinin-mediated cell adhe...