1. LRP8-Reelin-Regulated Neuronal Enhancer Signature Underlying Learning and Memory Formation.

    Neuron 86(3):696 (2015) PMID 25892301 PMCID PMC4486257

    One of the exceptional properties of the brain is its ability to acquire new knowledge through learning and to store that information through memory. The epigenetic mechanisms linking changes in neuronal transcriptional programs to behavioral plasticity remain largely unknown. Here, we identify ...
  2. Enhancer-bound LDB1 regulates a corticotrope promoter-pausing repression program.

    PNAS 112(5):1380 (2015) PMID 25605944 PMCID PMC4321301

    Substantial evidence supports the hypothesis that enhancers are critical regulators of cell-type determination, orchestrating both positive and negative transcriptional programs; however, the basic mechanisms by which enhancers orchestrate interactions with cognate promoters during activation an...
  3. Enhancer-bound LDB1 regulates a corticotrope promoter-pausing repression program.

    PNAS 112(5):1380 (2015) PMID 25605944

    Substantial evidence supports the hypothesis that enhancers are critical regulators of cell-type determination, orchestrating both positive and negative transcriptional programs; however, the basic mechanisms by which enhancers orchestrate interactions with cognate promoters during activation an...
  4. Enhancer-bound LDB1 regulates a corticotrope promoter-pausing repression program.

    PNAS 112(5):1380 (2015) PMID 25605944 PMCID PMC4321301

    Substantial evidence supports the hypothesis that enhancers are critical regulators of cell-type determination, orchestrating both positive and negative transcriptional programs; however, the basic mechanisms by which enhancers orchestrate interactions with cognate promoters during activation an...
  5. Enhancer-bound LDB1 regulates a corticotrope promoter-pausing repression program.

    PNAS 112(5):1380 (2015) PMID 25605944 PMCID PMC4321301

    Substantial evidence supports the hypothesis that enhancers are critical regulators of cell-type determination, orchestrating both positive and negative transcriptional programs; however, the basic mechanisms by which enhancers orchestrate interactions with cognate promoters during activation an...
  6. Enhancer-bound LDB1 regulates a corticotrope promoter-pausing repression program.

    PNAS 112(5):1380 (2015) PMID 25605944 PMCID PMC4321301

    Substantial evidence supports the hypothesis that enhancers are critical regulators of cell-type determination, orchestrating both positive and negative transcriptional programs; however, the basic mechanisms by which enhancers orchestrate interactions with cognate promoters during activation an...
  7. Ligand-dependent enhancer activation regulated by topoisomerase-I activity.

    Cell 160(3):367 (2015) PMID 25619691

    The discovery that enhancers are regulated transcription units, encoding eRNAs, has raised new questions about the mechanisms of their activation. Here, we report an unexpected molecular mechanism that underlies ligand-dependent enhancer activation, based on DNA nicking to relieve torsional stre...
  8. Ligand-dependent enhancer activation regulated by topoisomerase-I activity.

    Cell 160(3):367 (2015) PMID 25619691

    The discovery that enhancers are regulated transcription units, encoding eRNAs, has raised new questions about the mechanisms of their activation. Here, we report an unexpected molecular mechanism that underlies ligand-dependent enhancer activation, based on DNA nicking to relieve torsional stre...
  9. Ligand-dependent enhancer activation regulated by topoisomerase-I activity.

    Cell 160(3):367 (2015) PMID 25619691

    The discovery that enhancers are regulated transcription units, encoding eRNAs, has raised new questions about the mechanisms of their activation. Here, we report an unexpected molecular mechanism that underlies ligand-dependent enhancer activation, based on DNA nicking to relieve torsional stre...
  10. Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation.

    Nature 516(7530):267 (2014) PMID 25252977

    Post-translational histone modifications have a critical role in regulating transcription, the cell cycle, DNA replication and DNA damage repair. The identification of new histone modifications critical for transcriptional regulation at initiation, elongation or termination is of particular inte...
  11. Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation.

    Nature 516(7530):267 (2014) PMID 25252977 PMCID PMC4461219

    Post-translational histone modifications have a critical role in regulating transcription, the cell cycle, DNA replication and DNA damage repair. The identification of new histone modifications critical for transcriptional regulation at initiation, elongation or termination is of particular inte...
  12. Enhancer activation requires trans-recruitment of a mega transcription factor complex.

    Cell 159(2):358 (2014) PMID 25303530 PMCID PMC4465761

    Enhancers provide critical information directing cell-type-specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here, we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by tr...
  13. Enhancer activation requires trans-recruitment of a mega transcription factor complex.

    Cell 159(2):358 (2014) PMID 25303530

    Enhancers provide critical information directing cell-type-specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here, we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by tr...
  14. Required enhancer-matrin-3 network interactions for a homeodomain transcription program.

    Nature 514(7521):257 (2014) PMID 25119036 PMCID PMC4358797

    Homeodomain proteins, described 30 years ago, exert essential roles in development as regulators of target gene expression; however, the molecular mechanisms underlying transcriptional activity of homeodomain factors remain poorly understood. Here investigation of a developmentally required POU-...
  15. Required enhancer-matrin-3 network interactions for a homeodomain transcription program.

    Nature 514(7521):257 (2014) PMID 25119036 PMCID PMC4358797

    Homeodomain proteins, described 30 years ago, exert essential roles in development as regulators of target gene expression; however, the molecular mechanisms underlying transcriptional activity of homeodomain factors remain poorly understood. Here investigation of a developmentally required POU-...
  16. Ligand-dependent Enhancer Activation Regulated by Topoisomerase-I Activity

    Cell (2014)

    The discovery that enhancers are regulated transcription units, encoding eRNAs, has raised new questions about the mechanisms of their activation. Here, we report an unexpected molecular mechanism that underlies ligand-dependent enhancer activation, based on DNA nicking to relieve tors...
  17. Chem-seq permits identification of genomic targets of drugs against androgen receptor regulation selected by functional phenotypic screens.

    PNAS 111(25):9235 (2014) PMID 24928520 PMCID PMC4078819

    Understanding the mechanisms by which compounds discovered using cell-based phenotypic screening strategies might exert their effects would be highly augmented by new approaches exploring their potential interactions with the genome. For example, altered androgen receptor (AR) transcriptional pr...
  18. Chem-seq permits identification of genomic targets of drugs against androgen receptor regulation selected by functional phenotypic screens.

    PNAS 111(25):9235 (2014) PMID 24928520 PMCID PMC4078819

    Understanding the mechanisms by which compounds discovered using cell-based phenotypic screening strategies might exert their effects would be highly augmented by new approaches exploring their potential interactions with the genome. For example, altered androgen receptor (AR) transcriptional pr...
  19. ncRNA- and Pc2 Methylation-Dependent Gene Relocation between Nuclear Structures Mediates Gene Activation Programs

    Cell 155(2):478 (2013)

  20. lncRNA-dependent mechanisms of androgen-receptor-regulated gene activation programs.

    Nature 500(7464):598 (2013) PMID 23945587 PMCID PMC4034386

    Although recent studies have indicated roles of long non-coding RNAs (lncRNAs) in physiological aspects of cell-type determination and tissue homeostasis, their potential involvement in regulated gene transcription programs remains rather poorly understood. The androgen receptor regulates a larg...