1. Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion.

    Nucleic Acids Research 43(11):e73 (2015) PMID 25765642 PMCID PMC4477642

    With the aim of broadening the versatility of lentiviral vectors as a tool in nucleic acid research, we expanded the genetic code in the propagation of lentiviral vectors for site-specific incorporation of chemical moieties with unique properties. Through systematic exploration of the structure-...
  2. Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library.

    PLoS ONE 8(3):e59445 (2013) PMID 23527197 PMCID PMC3602433

    RNA interference (RNAi) is an endogenous RNA-destruction phenomenon induced by certain double-stranded RNAs (dsRNAs). In RNAi, dsRNAs are processed into small interfering RNAs (siRNAs) which in turn trigger the cleavage of the target mRNA. Here, using a short hairpin RNA-expression library, we i...
  3. Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library.

    PLoS ONE 8(3):e59445 (2013) PMID 23527197 PMCID PMC3602433

    RNA interference (RNAi) is an endogenous RNA-destruction phenomenon induced by certain double-stranded RNAs (dsRNAs). In RNAi, dsRNAs are processed into small interfering RNAs (siRNAs) which in turn trigger the cleavage of the target mRNA. Here, using a short hairpin RNA-expression library, we i...
  4. Crystallographic snapshots of an entire reaction cycle for a retaining xylanase from Streptomyces olivaceoviridis E-86.

    Journal of Biochemistry 146(1):61 (2009) PMID 19279191

    Retaining glycosyl hydrolases, which catalyse both glycosylation and deglycosylation in a concerted manner, are the most abundant hydrolases. To date, their visualization has tended to be focused on glycosylation because glycosylation reactions can be visualized by inactivating deglycosylation s...
  5. Crystallographic snapshots of an entire reaction cycle for a retaining xylanase from Streptomyces olivaceoviridis E-86.

    Journal of Biochemistry 146(1):61 (2009) PMID 19279191

    Retaining glycosyl hydrolases, which catalyse both glycosylation and deglycosylation in a concerted manner, are the most abundant hydrolases. To date, their visualization has tended to be focused on glycosylation because glycosylation reactions can be visualized by inactivating deglycosylation s...
  6. Loss-of-function screening by randomized intracellular antibodies: identification of hnRNP-K as a potential target for metastasis.

    PNAS 104(21):8983 (2007) PMID 17483488 PMCID PMC1885614

    We have developed a loss-of-function screening system on the basis of intracellular expression of single domain antibodies. We demonstrate its use in identification of potential targets of metastasis of human cancerous cells. Randomized intracellular antibodies were expressed in highly metastati...
  7. Loss-of-function screening by randomized intracellular antibodies: identification of hnRNP-K as a potential target for metastasis.

    PNAS 104(21):8983 (2007) PMID 17483488 PMCID PMC1885614

    We have developed a loss-of-function screening system on the basis of intracellular expression of single domain antibodies. We demonstrate its use in identification of potential targets of metastasis of human cancerous cells. Randomized intracellular antibodies were expressed in highly metastati...
  8. Genome-wide sreening by using small-interfering RNA expression libraries.

    Methods in Molecular Biology 360:131 (2007) PMID 17172728

    RNA interference (RNAi) is an evolutionarily conserved phenomenon in which gene expression is silenced by double-stranded RNA (dsRNA) in a sequence-specific manner. This technology has the potential to affect all aspects of target discovery and validation. With the completion of the human genome...
  9. Ribosome-inactivation display system.

    Methods in Molecular Biology 352:221 (2007) PMID 17041268

    We present a novel strategy for the connection of phenotype and genotype in vitro that can be used for the selection of functional proteins. The strategy involves the generation of a stable complex among a ribosome, an messenger RNA and its translated protein, without removal of the termination ...
  10. Genome-wide sreening by using small-interfering RNA expression libraries.

    Methods in Molecular Biology 360:131 (2007) PMID 17172728

    RNA interference (RNAi) is an evolutionarily conserved phenomenon in which gene expression is silenced by double-stranded RNA (dsRNA) in a sequence-specific manner. This technology has the potential to affect all aspects of target discovery and validation. With the completion of the human genome...
  11. Hammerhead ribozyme-based target discovery.

    Methods in Molecular Biology 360:143 (2007) PMID 17172729

    With the accumulation of vast amounts of data as a result of the sequencing of the human genome, it is necessary to identify human genes that are involved in various cellular, developmental, and disease-related processes and to clarify their functions and potential utility as targets in the trea...
  12. Ribosome-inactivation display system.

    Methods in Molecular Biology 352:221 (2007) PMID 17041268

    We present a novel strategy for the connection of phenotype and genotype in vitro that can be used for the selection of functional proteins. The strategy involves the generation of a stable complex among a ribosome, an messenger RNA and its translated protein, without removal of the termination ...
  13. Hammerhead ribozyme-based target discovery.

    Methods in Molecular Biology 360:143 (2007) PMID 17172729

    With the accumulation of vast amounts of data as a result of the sequencing of the human genome, it is necessary to identify human genes that are involved in various cellular, developmental, and disease-related processes and to clarify their functions and potential utility as targets in the trea...
  14. Leucyl/Phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids.

    ChemBioChem 7(11):1676 (2006) PMID 16977663

  15. Leucyl/Phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids.

    ChemBioChem 7(11):1676 (2006) PMID 16977663

  16. Retraction: Identification of genes that function in the TNF-alpha-mediated apoptotic pathway using randomized hybrid ribozyme libraries.

    Nature Biotechnology 24(9):1170 (2006) PMID 16964231

  17. Retraction: Identification of genes that function in the TNF-alpha-mediated apoptotic pathway using randomized hybrid ribozyme libraries.

    Nature Biotechnology 24(9):1170 (2006) PMID 16964231

  18. Ligand-targeted delivery of therapeutic siRNA.

    Pharmaceutical Research 23(8):1631 (2006) PMID 16850274

    RNA interference (RNAi) is a post-transcriptional gene-silencing phenomenon that is triggered by double-stranded RNA (dsRNA). Since many diseases are associated with the inappropriate production of specific proteins, attempts are being made to exploit RNAi in a clinical settings. However, before...
  19. Ligand-targeted delivery of therapeutic siRNA.

    Pharmaceutical Research 23(8):1631 (2006) PMID 16850274

    RNA interference (RNAi) is a post-transcriptional gene-silencing phenomenon that is triggered by double-stranded RNA (dsRNA). Since many diseases are associated with the inappropriate production of specific proteins, attempts are being made to exploit RNAi in a clinical settings. However, before...
  20. Identification of genes by hybrid ribozymes that couple cleavage activity with the unwinding activity of an endogenous RNA helicase.

    EMBO Reports 7(7):746 (2006) PMID 16819465 PMCID PMC1500838