1. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways

    Cell 160(5):870 (2015)

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. −1 frameshifting occurs on Escherichia coli’s dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process...
  2. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways.

    Cell 160(5):870 (2015) PMID 25703095 PMCID PMC4344849

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. -1 frameshifting occurs on Escherichia coli's dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process, includin...
  3. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways.

    Cell 160(5):870 (2015) PMID 25703095

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. -1 frameshifting occurs on Escherichia coli's dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process, includin...
  4. Folding a stable RNA pseudoknot through rearrangement of two hairpin structures.

    Nucleic Acids Research 42(7):4505 (2014) PMID 24459133 PMCID PMC3985624

    Folding messenger RNA into specific structures is a common regulatory mechanism involved in translation. In Escherichia coli, the operator of the rpsO gene transcript folds into a pseudoknot or double-hairpin conformation. S15, the gene product, binds only to the pseudoknot, thereby repressing i...
  5. Deciphering Ribosomal Frameshifting Dynamics

    Biophysical Journal 106(2):239a (2014)

  6. Study of Polyribosomal Effects on Frameshifting

    Biophysical Journal 106(2):493a (2014)

  7. Characterization of Ribosomal Bypassing through a 50-Nt mRNA Untranslated Gap

    Biophysical Journal 106(2):493a (2014)

  8. Direct measurement of the mechanical work during translocation by the ribosome.

    eLife 3:e03406 (2014) PMID 25114092 PMCID PMC4126342

    A detailed understanding of tRNA/mRNA translocation requires measurement of the forces generated by the ribosome during this movement. Such measurements have so far remained elusive and, thus, little is known about the relation between force and translocation and how this reflects on its mechani...
  9. Direct measurement of the mechanical work during translocation by the ribosome.

    eLife 3:e03406 (2014) PMID 25114092 PMCID PMC4126342

    A detailed understanding of tRNA/mRNA translocation requires measurement of the forces generated by the ribosome during this movement. Such measurements have so far remained elusive and, thus, little is known about the relation between force and translocation and how this reflects on its mechani...
  10. Single-Molecule Study on Structural Rearrangement of the 5’UTR ofrpsOMessenger RNA

    Biophysical Journal 104(2):259a (2013)

  11. Study of Helicase Activity of the Ribosome using Single-Molecule FRET

    Biophysical Journal 104(2):259a (2013)

  12. Mechanical Force during Translocation by the Ribosome

    Biophysical Journal 104(2):258a (2013)

  13. Deciphering Ribosomal Frameshifting Dynamics

    Biophysical Journal 102(3):68a (2012)

  14. Modelling Programmed Ribosomal Frameshifting with Elastic Network Model and Linear Response Theory

    Biophysical Journal 102(3):70a (2012)

  15. Study of Helicase Activity of the Ribosome using Single-Molecule FRET

    Biophysical Journal 102(3):68a (2012)

  16. The ribosome uses two active mechanisms to unwind messenger RNA during translation.

    Nature 475(7354):118 (2011) PMID 21734708 PMCID PMC4170678

    The ribosome translates the genetic information encoded in messenger RNA into protein. Folded structures in the coding region of an mRNA represent a kinetic barrier that lowers the peptide elongation rate, as the ribosome must disrupt structures it encounters in the mRNA at its entry site to all...
  17. Simulation and analysis of single-ribosome translation.

    Physical Biology 6(2):025006 (2009) PMID 19571367

    In the cell, proteins are synthesized by ribosomes in a multi-step process called translation. The ribosome translocates along the messenger RNA to read the codons that encode the amino acid sequence of a protein. Elongation factors, including EF-G and EF-Tu, are used to catalyze the process. Re...
  18. Following translation by single ribosomes one codon at a time.

    Nature 452(7187):598 (2008) PMID 18327250 PMCID PMC2556548

    We have followed individual ribosomes as they translate single messenger RNA hairpins tethered by the ends to optical tweezers. Here we reveal that translation occurs through successive translocation--and-pause cycles. The distribution of pause lengths, with a median of 2.8 s, indicates that at ...
  19. Measured and calculated CD spectra of G-quartets stacked with the same or opposite polarities.

    Chirality 20(3-4):431 (2008) PMID 17853398

    Circular dichroism (CD) spectroscopy is widely used to characterize the structures of DNA G-quadruplexes. CD bands at 200-300 nm have been empirically related to G-quadruplexes having parallel or antiparallel sugar-phosphate backbones. We propose that a more fundamental interpretation of the ori...
  20. Single-molecule mechanical unfolding and folding of a pseudoknot in human telomerase RNA.

    RNA 13(12):2175 (2007) PMID 17959928 PMCID PMC2080604

    RNA unfolding and folding reactions in physiological conditions can be facilitated by mechanical force one molecule at a time. By using force-measuring optical tweezers, we studied the mechanical unfolding and folding of a hairpin-type pseudoknot in human telomerase RNA in a near-physiological s...