1. Comprehensive transcriptome analysis using synthetic long-read sequencing reveals molecular co-association of distant splicing events.

    Nature Biotechnology 33(7):736 (2015) PMID 25985263

    Alternative splicing shapes mammalian transcriptomes, with many RNA molecules undergoing multiple distant alternative splicing events. Comprehensive transcriptome analysis, including analysis of exon co-association in the same molecule, requires deep, long-read sequencing. Here we introduce an R...
  2. A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate.

    Cell 160(5):1013 (2015) PMID 25684364 PMCID PMC4344913

    Aging is a complex process that affects multiple organs. Modeling aging and age-related diseases in the lab is challenging because classical vertebrate models have relatively long lifespans. Here, we develop the first platform for rapid exploration of age-dependent traits and diseases in vertebr...
  3. A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate.

    Cell 160(5):1013 (2015) PMID 25684364 PMCID PMC4344913

    Aging is a complex process that affects multiple organs. Modeling aging and age-related diseases in the lab is challenging because classical vertebrate models have relatively long lifespans. Here, we develop the first platform for rapid exploration of age-dependent traits and diseases in vertebr...
  4. A Platform for Rapid Exploration of Aging and Diseases in a Naturally Short-Lived Vertebrate

    Cell 160(5):1013 (2015)

    Aging is a complex process that affects multiple organs. Modeling aging and age-related diseases in the lab is challenging because classical vertebrate models have relatively long lifespans. Here, we develop the first platform for rapid exploration of age-dependent traits and diseases ...
  5. A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate.

    Cell 160(5):1013 (2015) PMID 25684364

    Aging is a complex process that affects multiple organs. Modeling aging and age-related diseases in the lab is challenging because classical vertebrate models have relatively long lifespans. Here, we develop the first platform for rapid exploration of age-dependent traits and diseases in vertebr...
  6. Pharyngeal mesoderm regulatory network controls cardiac and head muscle morphogenesis.

    PNAS 109(46):18839 (2012) PMID 23112163 PMCID PMC3503185

    The search for developmental mechanisms driving vertebrate organogenesis has paved the way toward a deeper understanding of birth defects. During embryogenesis, parts of the heart and craniofacial muscles arise from pharyngeal mesoderm (PM) progenitors. Here, we reveal a hierarchical regulatory ...
  7. Pharyngeal mesoderm regulatory network controls cardiac and head muscle morphogenesis.

    PNAS 109(46):18839 (2012) PMID 23112163 PMCID PMC3503185

    The search for developmental mechanisms driving vertebrate organogenesis has paved the way toward a deeper understanding of birth defects. During embryogenesis, parts of the heart and craniofacial muscles arise from pharyngeal mesoderm (PM) progenitors. Here, we reveal a hierarchical regulatory ...
  8. The actin regulator N-WASp is required for muscle-cell fusion in mice.

    PNAS 109(28):11211 (2012) PMID 22736793 PMCID PMC3396508

    A fundamental aspect of skeletal myogenesis involves extensive rounds of cell fusion, in which individual myoblasts are incorporated into growing muscle fibers. Here we demonstrate that N-WASp, a ubiquitous nucleation-promoting factor of branched microfilament arrays, is an essential contributor...
  9. The actin regulator N-WASp is required for muscle-cell fusion in mice.

    PNAS 109(28):11211 (2012) PMID 22736793 PMCID PMC3396508

    A fundamental aspect of skeletal myogenesis involves extensive rounds of cell fusion, in which individual myoblasts are incorporated into growing muscle fibers. Here we demonstrate that N-WASp, a ubiquitous nucleation-promoting factor of branched microfilament arrays, is an essential contributor...
  10. The occipital lateral plate mesoderm is a novel source for vertebrate neck musculature.

    Development 137(17):2961 (2010) PMID 20699298

    In vertebrates, body musculature originates from somites, whereas head muscles originate from the cranial mesoderm. Neck muscles are located in the transition between these regions. We show that the chick occipital lateral plate mesoderm has myogenic capacity and gives rise to large muscles loca...
  11. The occipital lateral plate mesoderm is a novel source for vertebrate neck musculature.

    Development 137(17):2961 (2010) PMID 20699298

    In vertebrates, body musculature originates from somites, whereas head muscles originate from the cranial mesoderm. Neck muscles are located in the transition between these regions. We show that the chick occipital lateral plate mesoderm has myogenic capacity and gives rise to large muscles loca...
  12. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis.

    Journal of Cell Biology 187(1):91 (2009) PMID 19786578 PMCID PMC2762088

    Merkel cells (MCs) are located in the touch-sensitive area of the epidermis and mediate mechanotransduction in the skin. Whether MCs originate from embryonic epidermal or neural crest progenitors has been a matter of intense controversy since their discovery >130 yr ago. In addition, how MCs are...
  13. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis.

    Journal of Cell Biology 187(1):91 (2009) PMID 19786578 PMCID PMC2762088

    Merkel cells (MCs) are located in the touch-sensitive area of the epidermis and mediate mechanotransduction in the skin. Whether MCs originate from embryonic epidermal or neural crest progenitors has been a matter of intense controversy since their discovery >130 yr ago. In addition, how MCs are...
  14. Distinct origins and genetic programs of head muscle satellite cells.

    Developmental Cell 16(6):822 (2009) PMID 19531353 PMCID PMC3684422

    Adult skeletal muscle possesses a remarkable regenerative capacity, due to the presence of satellite cells, adult muscle stem cells. We used fate-mapping techniques in avian and mouse models to show that trunk (Pax3(+)) and cranial (MesP1(+)) skeletal muscle and satellite cells derive from separ...
  15. Distinct origins and genetic programs of head muscle satellite cells.

    Developmental Cell 16(6):822 (2009) PMID 19531353 PMCID PMC3684422

    Adult skeletal muscle possesses a remarkable regenerative capacity, due to the presence of satellite cells, adult muscle stem cells. We used fate-mapping techniques in avian and mouse models to show that trunk (Pax3(+)) and cranial (MesP1(+)) skeletal muscle and satellite cells derive from separ...
  16. Distinct Origins and Genetic Programs of Head Muscle Satellite Cells

    Developmental Cell 16(6):822 (2009)

    Adult skeletal muscle possesses a remarkable regenerative capacity, due to the presence of satellite cells, adult muscle stem cells. We used fate-mapping techniques in avian and mouse models to show that trunk (Pax3 +) and cranial (MesP1 +) skeletal muscle and satellite cells de...
  17. The contribution of Islet1-expressing splanchnic mesoderm cells to distinct branchiomeric muscles reveals significant heterogeneity in head muscle development.

    Development 135(4):647 (2008) PMID 18184728

    During embryogenesis, paraxial mesoderm cells contribute skeletal muscle progenitors, whereas cardiac progenitors originate in the lateral splanchnic mesoderm (SpM). Here we focus on a subset of the SpM that contributes to the anterior or secondary heart field (AHF/SHF), and lies adjacent to the...
  18. The contribution of Islet1-expressing splanchnic mesoderm cells to distinct branchiomeric muscles reveals significant heterogeneity in head muscle development.

    Development 135(4):647 (2008) PMID 18184728

    During embryogenesis, paraxial mesoderm cells contribute skeletal muscle progenitors, whereas cardiac progenitors originate in the lateral splanchnic mesoderm (SpM). Here we focus on a subset of the SpM that contributes to the anterior or secondary heart field (AHF/SHF), and lies adjacent to the...