1. The ethical scientist: An old-fashioned view.

    Biopolymers 103(8):424 (2015) PMID 25269998 PMCID PMC4382459

    My personal view of ethical behavior as a scientific researcher in an academic environment is presented. I discuss the behavior of a graduate student, a postdoctoral, and a professor. Ethical behavior in teaching, choosing a research project, publishing papers, and obtaining a job is discussed. ...
  2. Ribosome. Mechanical force releases nascent chain-mediated ribosome arrest in vitro and in vivo.

    Science 348(6233):457 (2015) PMID 25908824

    Protein synthesis rates can affect gene expression and the folding and activity of the translation product. Interactions between the nascent polypeptide and the ribosome exit tunnel represent one mode of regulating synthesis rates. The SecM protein arrests its own translation, and release of arr...
  3. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways

    Cell 160(5):870 (2015)

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. −1 frameshifting occurs on Escherichia coli’s dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process...
  4. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways.

    Cell 160(5):870 (2015) PMID 25703095 PMCID PMC4344849

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. -1 frameshifting occurs on Escherichia coli's dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process, includin...
  5. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways.

    Cell 160(5):870 (2015) PMID 25703095 PMCID PMC4344849

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. -1 frameshifting occurs on Escherichia coli's dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process, includin...
  6. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways.

    Cell 160(5):870 (2015) PMID 25703095

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. -1 frameshifting occurs on Escherichia coli's dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process, includin...
  7. A frameshifting stimulatory stem loop destabilizes the hybrid state and impedes ribosomal translocation.

    PNAS 111(15):5538 (2014) PMID 24706807 PMCID PMC3992627

    Ribosomal frameshifting occurs when a ribosome slips a few nucleotides on an mRNA and generates a new sequence of amino acids. Programmed -1 ribosomal frameshifting (-1PRF) is used in various systems to express two or more proteins from a single mRNA at precisely regulated levels. We used single...
  8. A frameshifting stimulatory stem loop destabilizes the hybrid state and impedes ribosomal translocation.

    PNAS 111(15):5538 (2014) PMID 24706807 PMCID PMC3992627

    Ribosomal frameshifting occurs when a ribosome slips a few nucleotides on an mRNA and generates a new sequence of amino acids. Programmed -1 ribosomal frameshifting (-1PRF) is used in various systems to express two or more proteins from a single mRNA at precisely regulated levels. We used single...
  9. Probing the mechanisms of translation with force.

    Chemical Reviews 114(6):3266 (2014) PMID 24405158 PMCID PMC3968188

  10. Probing the mechanisms of translation with force.

    Chemical Reviews 114(6):3266 (2014) PMID 24405158 PMCID PMC3968188

  11. Probing the mechanisms of translation with force.

    Chemical Reviews 114(6):3266 (2014) PMID 24405158 PMCID PMC3968188

  12. Deciphering Ribosomal Frameshifting Dynamics

    Biophysical Journal 106(2):239a (2014)

  13. An mRNA Downstream Structure Promotes Frameshifting by Destabilizing the Hybrid State and Impeding the EF-G Driven Translocation Process

    Biophysical Journal 106(2):490a (2014)

  14. An mRNA Downstream Structure Promotes Frameshifting by Destabilizing the Hybrid State and Impeding the EF-G Driven Translocation Process

    Biophysical Journal 106(2):490a (2014)

  15. Direct measurement of the mechanical work during translocation by the ribosome.

    eLife 3:e03406 (2014) PMID 25114092 PMCID PMC4126342

    A detailed understanding of tRNA/mRNA translocation requires measurement of the forces generated by the ribosome during this movement. Such measurements have so far remained elusive and, thus, little is known about the relation between force and translocation and how this reflects on its mechani...
  16. Fun and games in Berkeley: the early years (1956-2013).

    Biophysics 43:1 (2014) PMID 24702008

    Life at Berkeley for the past 57 years involved research on the thermodynamics, kinetics, and spectroscopic properties of RNA to better understand its structures, interactions, and functions. We (myself and all the graduate students and postdocs who shared in the fun) began with dinucleoside pho...
  17. Fun and games in Berkeley: the early years (1956-2013).

    Biophysics 43:1 (2014) PMID 24702008

    Life at Berkeley for the past 57 years involved research on the thermodynamics, kinetics, and spectroscopic properties of RNA to better understand its structures, interactions, and functions. We (myself and all the graduate students and postdocs who shared in the fun) began with dinucleoside pho...
  18. Direct measurement of the mechanical work during translocation by the ribosome.

    eLife 3:e03406 (2014) PMID 25114092 PMCID PMC4126342

    A detailed understanding of tRNA/mRNA translocation requires measurement of the forces generated by the ribosome during this movement. Such measurements have so far remained elusive and, thus, little is known about the relation between force and translocation and how this reflects on its mechani...
  19. Direct measurement of the mechanical work during translocation by the ribosome.

    eLife 3:e03406 (2014) PMID 25114092 PMCID PMC4126342

    A detailed understanding of tRNA/mRNA translocation requires measurement of the forces generated by the ribosome during this movement. Such measurements have so far remained elusive and, thus, little is known about the relation between force and translocation and how this reflects on its mechani...
  20. Frameshifting dynamics.

    Biopolymers 99(12):1147 (2013) PMID 23722586 PMCID PMC4011568

    Translation of messenger RNA by a ribosome occurs three nucleotides at a time from start signal to stop. However, a frameshift means that some nucleotides are read twice or some are skipped, and the following sequence of amino acids is completely different from the sequence in the original frame...