Severe Meningoencephalitis in a Case of Ebola Virus Disease: A Case Report.
Annals of Internal Medicine 165(4):301 (2016)
Defective HIV-1 proviruses produce novel protein-coding RNA species in HIV-infected patients on combination antiretroviral therapy.
PNAS 113(31):8783 (2016)
Despite years of plasma HIV-RNA levels <40 copies per milliliter during combination antiretroviral therapy (cART), the majority of HIV-infected patients exhibit persistent seropositivity to HIV-1 and evidence of immune activation. These patients also show persistence of proviruses of HIV-1 in ci...
The pharmacist: An indispensable partner in HIV care.
American Journal of Health-System Pharmacy 73(7):431 (2016)
Conducting clinical trials in outbreak settings: Points to consider.
Clinical Trials 13(1):92 (2016)
Implementation of an Ebola virus disease vaccine clinical trial during the Ebola epidemic in Liberia: Design, procedures, and challenges.
Clinical Trials 13(1):49 (2016)
The index case of the Ebola virus disease epidemic in West Africa is believed to have originated in Guinea. By June 2014, Guinea, Liberia, and Sierra Leone were in the midst of a full-blown and complex global health emergency. The devastating effects of this Ebola epidemic in West Africa put the...
Discordance in lymphoid tissue recovery following stem cell transplantation in rhesus macaques: an in vivo imaging study.
Blood 126(24):2632 (2015)
Ionizing irradiation is used routinely to induce myeloablation and immunosuppression. However, it has not been possible to evaluate the extent of ablation without invasive biopsy. For lymphoid recovery, peripheral blood (PB) lymphocytes (PBLs) have been used for analysis, but they represent <2% ...
Initiation of Antiretroviral Therapy in Early Asymptomatic HIV Infection.
New England Journal of Medicine 373(9):795 (2015)
Data from randomized trials are lacking on the benefits and risks of initiating antiretroviral therapy in patients with asymptomatic human immunodeficiency virus (HIV) infection who have a CD4+ count of more than 350 cells per cubic millimeter.
We randomly assigned HIV-positive adults who had a ...
Characteristics and Clinical Management of a Cluster of 3 Patients With Ebola Virus Disease, Including the First Domestically Acquired Cases in the United States.
Annals of Internal Medicine 163(2):81 (2015)
More than 26,000 cases of Ebola virus disease (EVD) have been reported in western Africa, with high mortality. Several patients have been medically evacuated to hospitals in the United States and Europe. Detailed clinical data are limited on the clinical course and management of patients with EV...
Activated platelet-T-cell conjugates in peripheral blood of patients with HIV infection: coupling coagulation/inflammation and T cells.
AIDS 29(11):1297 (2015)
Despite successfully suppressed viremia by treatment, patients with high levels of biomarkers of coagulation/inflammation are at an increased risk of developing non-AIDS defining serious illnesses such as cardiovascular diseases. Thus, there is a relationship between persistent immune activation...
Emergency postexposure vaccination with vesicular stomatitis virus-vectored Ebola vaccine after needlestick.
JAMA 313(12):1249 (2015)
Safe and effective vaccines and drugs are needed for the prevention and treatment of Ebola virus disease, including following a potentially high-risk exposure such as a needlestick.
To assess response to postexposure vaccination in a health care worker who was exposed to the Ebola virus.
Redistribution, hyperproliferation, activation of natural killer cells and CD8 T cells, and cytokine production during first-in-human clinical trial of recombinant human interleukin-15 in patients with cancer.
Journal of Clinical Oncology 33(1):74 (2015)
Interleukin-15 (IL-15) has significant potential in cancer immunotherapy as an activator of antitumor CD8 T and natural killer (NK) cells. The primary objectives of this trial were to determine safety, adverse event profile, dose-limiting toxicity, and maximum-tolerated dose of recombinant human...
Lifespan of effector memory CD4+ T cells determined by replication-incompetent integrated HIV-1 provirus.
AIDS 28(8):1091 (2014)
Determining the precise lifespan of human T-cell is challenging due to the inability of standard techniques to distinguish between dividing and dying cells. Here, we measured the lifespan of a pool of T cells that were derived from a single cell 'naturally' labelled with a single integrated clon...
An update to the HIV-TRePS system: the development of new computational models that do not require a genotype to predict HIV treatment outcomes.
Journal of Antimicrobial Chemotherapy 69(4):1104 (2014)
The optimal individualized selection of antiretroviral drugs in resource-limited settings is challenging because of the limited availability of drugs and genotyping. Here we describe the development of the latest computational models to predict the response to combination antiretroviral therapy ...
Chronic exposure to type-I IFN under lymphopenic conditions alters CD4 T cell homeostasis.
PLoS Pathogens 10(3):e1003976 (2014)
HIV infection and the associated chronic immune activation alter T cell homeostasis leading to CD4 T cell depletion and CD8 T cell expansion. The mechanisms behind these outcomes are not totally defined and only partially explained by the direct cytopathic effect of the virus. In this manuscript...
A model of federal interagency cooperation: the National Interagency Confederation for Biological Research.
Biosecurity and Bioterrorism 12(3):144 (2014)
The terrorist attacks of September 11 and the anthrax mailings a month later prompted a sweeping response by the federal government to improve the preparedness of the US to meet the potential threat posed by a terrorist using a biological agent. This response transcended traditional interagency ...
siRNA enhances DNA-mediated interferon lambda-1 response through crosstalk between RIG-I and IFI16 signalling pathway.
Nucleic Acids Research 42(1):583 (2014)
In addition to silencing specific genes, small interfering RNA (siRNA) transfection is also associated with the non-specific induction of inflammatory cytokines and type I interferon. Those so-called "off-target" effects have considerable implications for the interpretation of in vitro studies a...
Outcomes of influenza A(H1N1)pdm09 virus infection: results from two international cohort studies.
PLoS ONE 9(7):e101785 (2014)
Data from prospectively planned cohort studies on risk of major clinical outcomes and prognostic factors for patients with influenza A(H1N1)pdm09 virus are limited. In 2009, in order to assess outcomes and evaluate risk factors for progression of illness, two cohort studies were initiated: FLU 0...
Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection.
PLoS ONE 9(6):e98989 (2014)
IL-27 is an immunomodulatory cytokine with potent anti-HIV properties in PBMCs, CD4+ T cells, macrophages and immature dendritic cells. Previous smaller studies have suggested that HIV-1 infection may alter IL-27 and influence HIV-1 pathogenesis. The aim of this study was to examine the relation...
Evaluating the potential of IL-27 as a novel therapeutic agent in HIV-1 infection.
Cytokine & Growth Factor Reviews 24(6):571 (2013)
Interleukin 27 (IL-27) is an immunomodulatory cytokine with important roles in both the innate and adaptive immune systems. In the last five years, the addition of exogenous IL-27 to primary cell cultures has been demonstrated to decrease HIV-1 replication in a number of cell types including per...
Cerebrospinal fluid HIV-1 compartmentalization in a patient with AIDS and acute varicella-zoster virus meningomyeloradiculitis.
Clinical Infectious Diseases 57(5):e135 (2013)
We report a case of AIDS presenting as varicella-zoster virus (VZV) meningomyeloradiculitis associated with human immunodeficiency virus (HIV) quasispecies compartmentalization within the cerebrospinal fluid (CSF), and a CSF viral load that was 1 log higher than in peripheral blood. Prolonged an...