1. Gut biogeography of the bacterial microbiota.

    Nature Reviews: Microbiology 14(1):20 (2016) PMID 26499895 PMCID PMC4837114

    Animals assemble and maintain a diverse but host-specific gut microbial community. In addition to characteristic microbial compositions along the longitudinal axis of the intestines, discrete bacterial communities form in microhabitats, such as the gut lumen, colonic mucus layers and colonic cry...
  2. Oligoribonuclease is the primary degradative enzyme for pGpG in Pseudomonas aeruginosa that is required for cyclic-di-GMP turnover.

    PNAS 112(36):E5048 (2015) PMID 26305945 PMCID PMC4568665

    The bacterial second messenger cyclic di-GMP (c-di-GMP) controls biofilm formation and other phenotypes relevant to pathogenesis. Cyclic-di-GMP is synthesized by diguanylate cyclases (DGCs). Phosphodiesterases (PDE-As) end signaling by linearizing c-di-GMP to 5'-phosphoguanylyl-(3',5')-guanosine...
  3. Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis.

    Cell 161(2):264 (2015) PMID 25860609 PMCID PMC4393509

    The gastrointestinal (GI) tract contains much of the body's serotonin (5-hydroxytryptamine, 5-HT), but mechanisms controlling the metabolism of gut-derived 5-HT remain unclear. Here, we demonstrate that the microbiota plays a critical role in regulating host 5-HT. Indigenous spore-forming bacter...
  4. Bacterial colonization factors control specificity and stability of the gut microbiota.

    Nature 501(7467):426 (2013) PMID 23955152 PMCID PMC3893107

    Mammals harbour a complex gut microbiome, comprising bacteria that confer immunological, metabolic and neurological benefits. Despite advances in sequence-based microbial profiling and myriad studies defining microbiome composition during health and disease, little is known about the molecular p...
  5. A rapid assay for affinity and kinetics of molecular interactions with nucleic acids.

    Nucleic Acids Research 40(7):e48 (2012) PMID 22210888 PMCID PMC3326337

    The Differential Radial Capillary Action of Ligand Assay (DRaCALA) allows detection of protein interactions with low-molecular weight ligands based on separation of the protein-ligand complex by differential capillary action. Here, we present an application of DRaCALA to the study of nucleic aci...
  6. Conservative change to the phosphate moiety of cyclic diguanylic monophosphate remarkably affects its polymorphism and ability to bind DGC, PDE, and PilZ proteins.

    Journal of the American Chemical Society 133(24):9320 (2011) PMID 21612220

    The cyclic dinucleotide c-di-GMP is a master regulator of bacterial virulence and biofilm formation. The activations of c-di-GMP metabolism proteins, diguanylate cyclases (DGCs) and phosophodiesterases (PDEs), usually lead to diametrically opposite phenotypes in bacteria. Analogues of c-di-GMP, ...