Plant cytokinesis-No ring, no constriction but centrifugal construction of the partitioning membrane.
Seminars in Cell & Developmental Biology 53:10 (2016)
Plants have evolved a unique way of partitioning the cytoplasm of dividing cells: Instead of forming a contractile ring that constricts the plasma membrane, plant cells target membrane vesicles to the plane of division where the vesicles fuse with one another to form the partitioning membrane. P...
Auxin responsiveness of the MONOPTEROS-BODENLOS module in primary root initiation critically depends on the nuclear import kinetics of the Aux/IAA inhibitor BODENLOS.
Plant Journal 85(2):269 (2016)
Primary root formation in early embryogenesis of Arabidopsis thaliana is initiated with the specification of a single cell called hypophysis. This initial step requires the auxin-dependent release of the transcription factor MONOPTEROS (MP, also known as ARF5) from its inhibition by the Aux/IAA ...
An S-Acylation Switch of Conserved G Domain Cysteines Is Required for Polarity Signaling by ROP GTPases
Current Biology 25(21):2875 (2015)
Profiling of embryonic nuclear vs. cellular RNA in Arabidopsis thaliana.
Genomics Data 4:96 (2015)
In Arabidopsis, various cell type-specific whole-genome expression analyses have been conducted. However, the vast majority of these were performed with cellular RNA from root tissues or other easily accessible cell types . Nuclear RNA was neglected for a long time as not being representative...
Plant cytokinesis: a tale of membrane traffic and fusion.
Biochemical Society Transactions 43(1):73 (2015)
Cytokinesis separates the forming daughter cells. Higher plants have lost the ability to constrict the plasma membrane (PM) in the division plane. Instead, trans-Golgi network (TGN)-derived membrane vesicles are targeted to the centre of the division plane and generate, by homotypic fusion, the ...
Twin plants from supernumerary egg cells in Arabidopsis.
Current Biology 25(2):225 (2015)
Sexual reproduction of flowering plants is distinguished by double fertilization—the two sperm cells delivered by a pollen tube fuse with the two gametic cells of the female gametophyte, the egg and the central cell—inside the ovule to give rise to the embryo and the nutritive endosperm, respect...
High lipid order of Arabidopsis cell-plate membranes mediated by sterol and DYNAMIN-RELATED PROTEIN1A function.
Plant Journal 80(5):745 (2014)
Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant...
Cell type-specific transcriptome analysis in the early Arabidopsis thaliana embryo.
Development 141(24):4831 (2014)
In multicellular organisms, cellular differences in gene activity are a prerequisite for differentiation and establishment of cell types. In order to study transcriptome profiles, specific cell types have to be isolated from a given tissue or even the whole organism. However, whole-transcriptome...
Current Biology 24(19):R944 (2014)
Current Biology 24(19) (2014)
Gerd Jürgens is Professor of Developmental Genetics at the Center for Plant Molecular Biology (ZMBP), University of Tübingen, and a Director at the Max Planck Institute for Developmental Biology, Tübingen, Germany.
Protein delivery to vacuole requires SAND protein-dependent Rab GTPase conversion for MVB-vacuole fusion.
Current Biology 24(12):1383 (2014)
Plasma-membrane proteins such as ligand-binding receptor kinases, ion channels, or nutrient transporters are turned over by targeting to a lytic compartment--lysosome or vacuole--for degradation. After their internalization, these proteins arrive at an early endosome, which then matures into a l...
Delivery of endocytosed proteins to the cell-division plane requires change of pathway from recycling to secretion.
eLife 3:e02131 (2014)
Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify ...
Plant secretome - from cellular process to biological activity.
Biochimica et Biophysica Acta 1834(11):2429 (2013)
Recent studies suggest that plants secrete a large number of proteins and peptides into the extracellular space. Secreted proteins play a crucial role in stress response, communication and development of organisms. Here we review the current knowledge of the secretome of more than ten plant spec...
Transcriptional repression of BODENLOS by HD-ZIP transcription factor HB5 in Arabidopsis thaliana.
Journal of Experimental Botany 64(10):3009 (2013)
In Arabidopsis thaliana, the phytohormone auxin is an important patterning agent during embryogenesis and post-embryonic development, exerting effects through transcriptional regulation. The main determinants of the transcriptional auxin response machinery are AUXIN RESPONSE FACTOR (ARF) transcr...
Arabidopsis μ-adaptin subunit AP1M of adaptor protein complex 1 mediates late secretory and vacuolar traffic and is required for growth.
PNAS 110(25):10318 (2013)
Adaptor protein (AP) complexes are the predominant coat proteins of membrane vesicles in post-Golgi trafficking of mammalian cells. Each AP complex contains a specific medium subunit, μ-adaptin, that selects cargo proteins bearing sequence-specific sorting motifs. Much less is known about the AP...
SNARE complexes of different composition jointly mediate membrane fusion in Arabidopsis cytokinesis.
Molecular Biology of the Cell 24(10):1593 (2013)
Membrane fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes. Although membrane fusion is required for separating daughter cells in eukaryotic cytokinesis, the SNARE complexes involved are not known. In plants, membrane vesicles targeted ...
Sec1/Munc18 Protein Stabilizes Fusion-Competent Syntaxin for Membrane Fusion in Arabidopsis Cytokinesis
Developmental Cell 22(5):989 (2012)
Intracellular membrane fusion requires complexes of syntaxins with other SNARE proteins and regulatory Sec1/Munc18 (SM) proteins. In membrane fusion mediating, e.g., neurotransmitter release or glucose-stimulated insulin secretion in mammals, SM proteins preferentially interact with th...
Arabidopsis WD repeat domain55 Interacts with DNA damaged binding protein1 and is required for apical patterning in the embryo.
Plant Cell 24(3):1013 (2012)
CUL4-RING ubiquitin E3 ligases (CRL4s) were recently shown to exert their specificity through the binding of various substrate receptors, which bind the CUL4 interactor DNA damaged binding protein1 (DDB1) through a WDxR motif. In a segregation-based mutagenesis screen, we identified a WDxR motif...
Different auxin response machineries control distinct cell fates in the early plant embryo.
Developmental Cell 22(1):211 (2012)
The cell types of the plant root are first specified early during embryogenesis and are maintained throughout plant life. Auxin plays an essential role in embryonic root initiation, in part through the action of the ARF5/MP transcription factor and its auxin-labile inhibitor IAA12/BDL. MP and BD...
Early embryogenesis in flowering plants: setting up the basic body pattern.
Plant Biology 63:483 (2012)
Early embryogenesis is the critical developmental phase during which the basic features of the plant body are established: the apical-basal axis of polarity, different tissue layers, and both the root pole and the shoot pole. Polarization of the zygote correlates with the generation of apical an...