1. Casein Kinase 1 and Phosphorylation of Cohesin Subunit Rec11 (SA3) Promote Meiotic Recombination through Linear Element Formation.

    PLoS Genetics 11(5):e1005225 (2015) PMID 25993311 PMCID PMC4439085

    Proper meiotic chromosome segregation, essential for sexual reproduction, requires timely formation and removal of sister chromatid cohesion and crossing-over between homologs. Early in meiosis cohesins hold sisters together and also promote formation of DNA double-strand breaks, obligate precur...
  2. Control of RecBCD enzyme activity by DNA binding- and Chi hotspot-dependent conformational changes.

    Journal of Molecular Biology 426(21):3479 (2014) PMID 25073102 PMCID PMC4188757

    Faithful repair of DNA double-strand breaks by homologous recombination is crucial to maintain functional genomes. The major Escherichia coli pathway of DNA break repair requires RecBCD enzyme, a complex protein machine with multiple activities. Upon encountering a Chi recombination hotspot (5' ...
  3. Control of RecBCD enzyme activity by DNA binding- and Chi hotspot-dependent conformational changes.

    Journal of Molecular Biology 426(21):3479 (2014) PMID 25073102 PMCID PMC4188757

    Faithful repair of DNA double-strand breaks by homologous recombination is crucial to maintain functional genomes. The major Escherichia coli pathway of DNA break repair requires RecBCD enzyme, a complex protein machine with multiple activities. Upon encountering a Chi recombination hotspot (5' ...
  4. Control of RecBCD enzyme activity by DNA binding- and Chi hotspot-dependent conformational changes.

    Journal of Molecular Biology 426(21):3479 (2014) PMID 25073102 PMCID PMC4188757

    Faithful repair of DNA double-strand breaks by homologous recombination is crucial to maintain functional genomes. The major Escherichia coli pathway of DNA break repair requires RecBCD enzyme, a complex protein machine with multiple activities. Upon encountering a Chi recombination hotspot (5' ...
  5. Evolutionarily diverse determinants of meiotic DNA break and recombination landscapes across the genome.

    Genome Research 24(10):1650 (2014) PMID 25024163 PMCID PMC4199369

    Fission yeast Rec12 (Spo11 homolog) initiates meiotic recombination by forming developmentally programmed DNA double-strand breaks (DSBs). DSB distributions influence patterns of heredity and genome evolution, but the basis of the highly nonrandom choice of Rec12 cleavage sites is poorly underst...
  6. Evolutionarily diverse determinants of meiotic DNA break and recombination landscapes across the genome.

    Genome Research 24(10):1650 (2014) PMID 25024163 PMCID PMC4199369

    Fission yeast Rec12 (Spo11 homolog) initiates meiotic recombination by forming developmentally programmed DNA double-strand breaks (DSBs). DSB distributions influence patterns of heredity and genome evolution, but the basis of the highly nonrandom choice of Rec12 cleavage sites is poorly underst...
  7. Evolutionarily diverse determinants of meiotic DNA break and recombination landscapes across the genome.

    Genome Research 24(10):1650 (2014) PMID 25024163 PMCID PMC4199369

    Fission yeast Rec12 (Spo11 homolog) initiates meiotic recombination by forming developmentally programmed DNA double-strand breaks (DSBs). DSB distributions influence patterns of heredity and genome evolution, but the basis of the highly nonrandom choice of Rec12 cleavage sites is poorly underst...
  8. Influence of introgression and geological processes on phylogenetic relationships of Western North American mountain suckers (Pantosteus, Catostomidae).

    PLoS ONE 9(3):e90061 (2014) PMID 24619087 PMCID PMC3949674

    Intense geological activity caused major topographic changes in Western North America over the past 15 million years. Major rivers here are composites of different ancient rivers, resulting in isolation and mixing episodes between river basins over time. This history influenced the diversificati...
  9. DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature.

    Nucleic Acids Research 42(1):359 (2014) PMID 24089141 PMCID PMC3874177

    Crossovers formed by recombination between homologous chromosomes are important for proper homolog segregation during meiosis and for generation of genetic diversity. Optimal molecular analysis of DNA intermediates of recombination requires synchronous cultures. We previously described a mutant,...
  10. Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast.

    eLife 3:e02630 (2014) PMID 24963140 PMCID PMC4066438

    Hybrid sterility is one of the earliest postzygotic isolating mechanisms to evolve between two recently diverged species. Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha, which mate to form viable hybr...
  11. Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase.

    Nature Protocols 9(1):223 (2014) PMID 24385151 PMCID PMC4117242

    Synchronous cultures are often indispensable for studying meiosis. Here we present an optimized protocol for induction of synchronous meiosis in the fission yeast Schizosaccharomyces pombe. Chemical inactivation of an ATP analog-sensitive form of the Pat1 kinase (pat1-as2) by adding the ATP anal...
  12. Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast.

    eLife 3:e02630 (2014) PMID 24963140 PMCID PMC4066438

    Hybrid sterility is one of the earliest postzygotic isolating mechanisms to evolve between two recently diverged species. Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha, which mate to form viable hybr...
  13. DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature.

    Nucleic Acids Research 42(1):359 (2014) PMID 24089141 PMCID PMC3874177

    Crossovers formed by recombination between homologous chromosomes are important for proper homolog segregation during meiosis and for generation of genetic diversity. Optimal molecular analysis of DNA intermediates of recombination requires synchronous cultures. We previously described a mutant,...
  14. Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase.

    Nature Protocols 9(1):223 (2014) PMID 24385151 PMCID PMC4117242

    Synchronous cultures are often indispensable for studying meiosis. Here we present an optimized protocol for induction of synchronous meiosis in the fission yeast Schizosaccharomyces pombe. Chemical inactivation of an ATP analog-sensitive form of the Pat1 kinase (pat1-as2) by adding the ATP anal...
  15. Influence of introgression and geological processes on phylogenetic relationships of Western North American mountain suckers (Pantosteus, Catostomidae).

    PLoS ONE 9(3):e90061 (2014) PMID 24619087 PMCID PMC3949674

    Intense geological activity caused major topographic changes in Western North America over the past 15 million years. Major rivers here are composites of different ancient rivers, resulting in isolation and mixing episodes between river basins over time. This history influenced the diversificati...
  16. Making chromosomes hot for breakage.

    Cell Cycle 12(9):1327 (2013) PMID 23588069 PMCID PMC3674054

  17. Making chromosomes hot for breakage.

    Cell Cycle 12(9):1327 (2013) PMID 23588069 PMCID PMC3674054

  18. Protein Determinants of Meiotic DNA Break Hot Spots

    Molecular Cell 49(5):983 (2013)

    Meiotic recombination, crucial for proper chromosome segregation and genome evolution, is initiated by programmed DNA double-strand breaks (DSBs) in yeasts and likely all sexually reproducing species. In fission yeast, DSBs occur up to hundreds of times more frequently at special sites...
  19. Protein determinants of meiotic DNA break hot spots.

    Molecular Cell 49(5):983 (2013) PMID 23395004 PMCID PMC3595357

    Meiotic recombination, crucial for proper chromosome segregation and genome evolution, is initiated by programmed DNA double-strand breaks (DSBs) in yeasts and likely all sexually reproducing species. In fission yeast, DSBs occur up to hundreds of times more frequently at special sites, called h...
  20. Protein determinants of meiotic DNA break hot spots.

    Molecular Cell 49(5):983 (2013) PMID 23395004 PMCID PMC3595357

    Meiotic recombination, crucial for proper chromosome segregation and genome evolution, is initiated by programmed DNA double-strand breaks (DSBs) in yeasts and likely all sexually reproducing species. In fission yeast, DSBs occur up to hundreds of times more frequently at special sites, called h...