1. Nuclear-Receptor-Mediated Telomere Insertion Leads to Genome Instability in ALT Cancers

    Cell 160(5):913 (2015)

    The breakage-fusion-bridge cycle is a classical mechanism of telomere-driven genome instability in which dysfunctional telomeres are fused to other chromosomal extremities, creating dicentric chromosomes that eventually break at mitosis. Here, we uncover a distinct pathway of telomere-...
  2. Nuclear-Receptor-Mediated Telomere Insertion Leads to Genome Instability in ALT Cancers

    Cell 160(5):913 (2015) PMID 25723166

    The breakage-fusion-bridge cycle is a classical mechanism of telomere-driven genome instability in which dysfunctional telomeres are fused to other chromosomal extremities, creating dicentric chromosomes that eventually break at mitosis. Here, we uncover a distinct pathway of telomere-...
  3. Nuclear-Receptor-Mediated Telomere Insertion Leads to Genome Instability in ALT Cancers.

    Cell 160(5):913 (2015) PMID 25723166

    The breakage-fusion-bridge cycle is a classical mechanism of telomere-driven genome instability in which dysfunctional telomeres are fused to other chromosomal extremities, creating dicentric chromosomes that eventually break at mitosis. Here, we uncover a distinct pathway of telomere-driven gen...
  4. Long lasting control of viral rebound with a new drug ABX464 targeting Rev - mediated viral RNA biogenesis.

    Retrovirology 12:30 (2015) PMID 25889234 PMCID PMC4422473

    Current therapies have succeeded in controlling AIDS pandemic. However, there is a continuing need for new drugs, in particular those acting through new and as yet unexplored mechanisms of action to achieve HIV infection cure. We took advantage of the unique feature of proviral genome to require...
  5. Stable assembly of HIV-1 export complexes occurs cotranscriptionally.

    RNA 20(1):1 (2014) PMID 24255166 PMCID PMC3866638

    The HIV-1 Rev protein mediates export of unspliced and singly spliced viral transcripts by binding to the Rev response element (RRE) and recruiting the cellular export factor CRM1. Here, we investigated the recruitment of Rev to the transcription sites of HIV-1 reporters that splice either post-...
  6. Stable assembly of HIV-1 export complexes occurs cotranscriptionally.

    RNA 20(1):1 (2014) PMID 24255166 PMCID PMC3866638

    The HIV-1 Rev protein mediates export of unspliced and singly spliced viral transcripts by binding to the Rev response element (RRE) and recruiting the cellular export factor CRM1. Here, we investigated the recruitment of Rev to the transcription sites of HIV-1 reporters that splice either post-...
  7. Stable assembly of HIV-1 export complexes occurs cotranscriptionally.

    RNA 20(1):1 (2014) PMID 24255166 PMCID PMC3866638

    The HIV-1 Rev protein mediates export of unspliced and singly spliced viral transcripts by binding to the Rev response element (RRE) and recruiting the cellular export factor CRM1. Here, we investigated the recruitment of Rev to the transcription sites of HIV-1 reporters that splice either post-...
  8. Stable assembly of HIV-1 export complexes occurs cotranscriptionally.

    RNA 20(1):1 (2014) PMID 24255166 PMCID PMC3866638

    The HIV-1 Rev protein mediates export of unspliced and singly spliced viral transcripts by binding to the Rev response element (RRE) and recruiting the cellular export factor CRM1. Here, we investigated the recruitment of Rev to the transcription sites of HIV-1 reporters that splice either post-...
  9. FISH-quant: automatic counting of transcripts in 3D FISH images.

    Nature Methods 10(4):277 (2013) PMID 23538861

  10. FISH-quant: automatic counting of transcripts in 3D FISH images.

    Nature Methods 10(4):277 (2013) PMID 23538861

  11. Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation.

    Journal of Cell Biology 193(5):819 (2011) PMID 21624952 PMCID PMC3105549

    Splicing is a key process that expands the coding capacity of genomes. Its kinetics remain poorly characterized, and the distribution of splicing time caused by the stochasticity of single splicing events is expected to affect regulation efficiency. We conducted a small-scale survey on 40 intron...
  12. Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation.

    Journal of Cell Biology 193(5):819 (2011) PMID 21624952 PMCID PMC3105549

    Splicing is a key process that expands the coding capacity of genomes. Its kinetics remain poorly characterized, and the distribution of splicing time caused by the stochasticity of single splicing events is expected to affect regulation efficiency. We conducted a small-scale survey on 40 intron...
  13. Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation.

    Journal of Cell Biology 193(5):819 (2011) PMID 21624952 PMCID PMC3105549

    Splicing is a key process that expands the coding capacity of genomes. Its kinetics remain poorly characterized, and the distribution of splicing time caused by the stochasticity of single splicing events is expected to affect regulation efficiency. We conducted a small-scale survey on 40 intron...
  14. Endosomal trafficking of HIV-1 gag and genomic RNAs regulates viral egress.

    Journal of Biological Chemistry 284(29):19727 (2009) PMID 19451649 PMCID PMC2740597

    HIV-1 Gag can assemble and generate virions at the plasma membrane, but it is also present in endosomes where its role remains incompletely characterized. Here, we show that HIV-1 RNAs and Gag are transported on endosomal vesicles positive for TiVamp, a v-SNARE involved in fusion events with the...
  15. Endosomal trafficking of HIV-1 gag and genomic RNAs regulates viral egress.

    Journal of Biological Chemistry 284(29):19727 (2009) PMID 19451649 PMCID PMC2740597

    HIV-1 Gag can assemble and generate virions at the plasma membrane, but it is also present in endosomes where its role remains incompletely characterized. Here, we show that HIV-1 RNAs and Gag are transported on endosomal vesicles positive for TiVamp, a v-SNARE involved in fusion events with the...
  16. The transcriptional cycle of HIV-1 in real-time and live cells.

    Journal of Cell Biology 179(2):291 (2007) PMID 17954611 PMCID PMC2064765

    RNA polymerase II (RNAPII) is a fundamental enzyme, but few studies have analyzed its activity in living cells. Using human immunodeficiency virus (HIV) type 1 reporters, we study real-time messenger RNA (mRNA) biogenesis by photobleaching nascent RNAs and RNAPII at specific transcription sites....
  17. The transcriptional cycle of HIV-1 in real-time and live cells.

    Journal of Cell Biology 179(2):291 (2007) PMID 17954611 PMCID PMC2064765

    RNA polymerase II (RNAPII) is a fundamental enzyme, but few studies have analyzed its activity in living cells. Using human immunodeficiency virus (HIV) type 1 reporters, we study real-time messenger RNA (mRNA) biogenesis by photobleaching nascent RNAs and RNAPII at specific transcription sites....
  18. Bsr, a nuclear-retained RNA with monoallelic expression.

    Molecular Biology of the Cell 18(8):2817 (2007) PMID 17507654 PMCID PMC1949380

    The imprinted Dlk1-Gtl2 and Prader-Willi syndrome (PWS) regions are characterized by a complex noncoding transcription unit spanning arrays of tandemly repeated C/D RNA genes. These noncoding RNAs (ncRNAs) are thought to play an essential but still poorly understood role. To better understand th...
  19. The clathrin adaptor complex AP-1 binds HIV-1 and MLV Gag and facilitates their budding.

    Molecular Biology of the Cell 18(8):3193 (2007) PMID 17538020 PMCID PMC1949356

    Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the clathrin adaptor complex AP-1 is involved in retroviral release. The absence of AP-1mu obtained by...
  20. The clathrin adaptor complex AP-1 binds HIV-1 and MLV Gag and facilitates their budding.

    Molecular Biology of the Cell 18(8):3193 (2007) PMID 17538020 PMCID PMC1949356

    Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the clathrin adaptor complex AP-1 is involved in retroviral release. The absence of AP-1mu obtained by...