1. ALT telomeres get together with nuclear receptors.

    Cell 160(5):811 (2015) PMID 25723159

    Nuclear receptors bind chromosome ends in "alternative lengthening of telomeres" (ALT) cancer cells that maintain their ends by homologous recombination instead of telomerase. Marzec et al. now demonstrate that, in ALT cells, nuclear receptors not only trigger distal chromatin associations to me...
  2. ALT Telomeres Get Together with Nuclear Receptors

    Cell 160(5):811 (2015)

    Nuclear receptors bind chromosome ends in “alternative lengthening of telomeres” (ALT) cancer cells that maintain their ends by homologous recombination instead of telomerase. Marzec et al. now demonstrate that, in ALT cells, nuclear receptors not only trigger distal chromatin associat...
  3. ALT Telomeres Get Together with Nuclear Receptors.

    Cell 160(5):811 (2015) PMID 25723159

    Nuclear receptors bind chromosome ends in "alternative lengthening of telomeres" (ALT) cancer cells that maintain their ends by homologous recombination instead of telomerase. Marzec et al. now demonstrate that, in ALT cells, nuclear receptors not only trigger distal chromatin associations to me...
  4. A quantitative telomeric chromatin isolation protocol identifies different telomeric states.

    Nature Communications 4:2848 (2013) PMID 24270157

    Telomere composition changes during tumourigenesis, aging and in telomere syndromes in a poorly defined manner. Here we develop a quantitative telomeric chromatin isolation protocol (QTIP) for human cells, in which chromatin is cross-linked, immunopurified and analysed by mass spectrometry. QTIP...
  5. A quantitative telomeric chromatin isolation protocol identifies different telomeric states.

    Nature Communications 4:2848 (2013) PMID 24270157

    Telomere composition changes during tumourigenesis, aging and in telomere syndromes in a poorly defined manner. Here we develop a quantitative telomeric chromatin isolation protocol (QTIP) for human cells, in which chromatin is cross-linked, immunopurified and analysed by mass spectrometry. QTIP...
  6. tRNASec is transcribed by RNA polymerase II in Trypanosoma brucei but not in humans.

    Nucleic Acids Research 38(17):5833 (2010) PMID 20444878 PMCID PMC2943599

    Nuclear-encoded tRNAs are universally transcribed by RNA polymerase III (Pol-III) and contain intragenic promoters. Transcription of vertebrate tRNA(Sec) however requires extragenic promoters similar to Pol-III transcribed U6 snRNA. Here, we present a comparative analysis of tRNA(Sec) transcript...
  7. tRNASec is transcribed by RNA polymerase II in Trypanosoma brucei but not in humans.

    Nucleic Acids Research 38(17):5833 (2010) PMID 20444878 PMCID PMC2943599

    Nuclear-encoded tRNAs are universally transcribed by RNA polymerase III (Pol-III) and contain intragenic promoters. Transcription of vertebrate tRNA(Sec) however requires extragenic promoters similar to Pol-III transcribed U6 snRNA. Here, we present a comparative analysis of tRNA(Sec) transcript...
  8. Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNA Met formylation in Apicomplexa.

    Molecular Microbiology 76(3):706 (2010) PMID 20374492

    Apicomplexans possess three translationally active compartments: the cytosol, a single tubular mitochondrion, and a vestigial plastid organelle called apicoplast. Mitochondrion and apicoplast are of bacterial evolutionary origin and therefore depend on a bacterial-like translation machinery. The...
  9. Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNA Met formylation in Apicomplexa.

    Molecular Microbiology 76(3):706 (2010) PMID 20374492

    Apicomplexans possess three translationally active compartments: the cytosol, a single tubular mitochondrion, and a vestigial plastid organelle called apicoplast. Mitochondrion and apicoplast are of bacterial evolutionary origin and therefore depend on a bacterial-like translation machinery. The...
  10. The selenoproteome is dispensable in bloodstream forms of Trypanosoma brucei.

    Molecular and Biochemical Parasitology 168(2):191 (2009) PMID 19723543

    Here we show that absence of Sep-tRNA:Sec-tRNA synthase (SepSecS) a key enzyme required for the synthesis of the three trypanosomal selenoproteins does not affect growth of bloodstream forms of Trypanosoma brucei. Both life cycle stages of T. brucei are highly sensitive to auranofin, a compound ...
  11. The canonical pathway for selenocysteine insertion is dispensable in Trypanosomes.

    PNAS 106(13):5088 (2009) PMID 19279205 PMCID PMC2664009

    The micronutrient selenium is found in proteins as selenocysteine (Sec), the 21st amino acid cotranslationally inserted in response to a UGA codon. In vitro studies in archaea and mouse showed that Sec-tRNA(Sec) formation is a 3-step process starting with serylation of tRNA(Sec) by seryl-tRNA sy...
  12. The canonical pathway for selenocysteine insertion is dispensable in Trypanosomes.

    PNAS 106(13):5088 (2009) PMID 19279205 PMCID PMC2664009

    The micronutrient selenium is found in proteins as selenocysteine (Sec), the 21st amino acid cotranslationally inserted in response to a UGA codon. In vitro studies in archaea and mouse showed that Sec-tRNA(Sec) formation is a 3-step process starting with serylation of tRNA(Sec) by seryl-tRNA sy...
  13. The selenoproteome is dispensable in bloodstream forms ofTrypanosoma brucei

    Molecular and Biochemical Parasitology 168(2):191 (2009)

    Here we show that absence of Sep-tRNA:Sec-tRNA synthase (SepSecS) a key enzyme required for the synthesis of the three trypanosomal selenoproteins does not affect growth of bloodstream forms of Trypanosoma brucei. Both life cycle stages of T. brucei are highly sensitive to aur...
  14. The selenoproteome is dispensable in bloodstream forms ofTrypanosoma brucei

    Molecular and Biochemical Parasitology 168(2):191 (2009) PMID 19723543

    Here we show that absence of Sep-tRNA:Sec-tRNA synthase (SepSecS) a key enzyme required for the synthesis of the three trypanosomal selenoproteins does not affect growth of bloodstream forms of Trypanosoma brucei. Both life cycle stages of T. brucei are highly sensitive to aur...
  15. The selenoproteome is dispensable in bloodstream forms ofTrypanosoma brucei

    Molecular and Biochemical Parasitology 168(2):191 (2009) PMID 19723543

    Here we show that absence of Sep-tRNA:Sec-tRNA synthase (SepSecS) a key enzyme required for the synthesis of the three trypanosomal selenoproteins does not affect growth of bloodstream forms of Trypanosoma brucei. Both life cycle stages of T. brucei are highly sensitive to aur...
  16. Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei.

    EMBO Journal 26(20):4302 (2007) PMID 17853889 PMCID PMC2034667

    Mitochondrial tRNA import is widespread in eukaryotes. Yet, the mechanism that determines its specificity is unknown. Previous in vivo experiments using the tRNAs(Met), tRNA(Ile) and tRNA(Lys) have suggested that the T-stem nucleotide pair 51:63 is the main localization determinant of tRNAs in T...
  17. Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei.

    EMBO Journal 26(20):4302 (2007) PMID 17853889 PMCID PMC2034667

    Mitochondrial tRNA import is widespread in eukaryotes. Yet, the mechanism that determines its specificity is unknown. Previous in vivo experiments using the tRNAs(Met), tRNA(Ile) and tRNA(Lys) have suggested that the T-stem nucleotide pair 51:63 is the main localization determinant of tRNAs in T...
  18. Trypanosoma seryl-tRNA synthetase is a metazoan-like enzyme with high affinity for tRNASec.

    Journal of Biological Chemistry 281(50):38217 (2006) PMID 17040903

    Trypanosomatids are important human pathogens that form a basal branch of eukaryotes. Their evolutionary history is still unclear as are many aspects of their molecular biology. Here we characterize essential components required for the incorporation of serine and selenocysteine into the proteom...
    PDF not found
  19. Trypanosoma seryl-tRNA synthetase is a metazoan-like enzyme with high affinity for tRNASec.

    Journal of Biological Chemistry 281(50):38217 (2006) PMID 17040903

    Trypanosomatids are important human pathogens that form a basal branch of eukaryotes. Their evolutionary history is still unclear as are many aspects of their molecular biology. Here we characterize essential components required for the incorporation of serine and selenocysteine into the proteom...
    PDF not found