1. The yeast Saccharomyces cerevisiae: An overview of methods to study autophagy progression

    Methods 75:3 (2015) PMID 25526918

    Macroautophagy (hereafter autophagy) is a highly evolutionarily conserved process essential for sustaining cellular integrity, homeostasis, and survival. Most eukaryotic cells constitutively undergo autophagy at a low basal level. However, various stimuli, including starvation, organel...
  2. Assays for the biochemical and ultrastructural measurement of selective and nonselective types of autophagy in the yeast Saccharomyces cerevisiae

    Methods 75:141 (2015) PMID 25484341

    Autophagy is a conserved intracellular catabolic pathway that degrades unnecessary or dysfunctional cellular components. Components destined for degradation are sequestered into double-membrane vesicles called autophagosomes, which subsequently fuse with the vacuole/lysosome delivering...
  3. Rph1/KDM4 Mediates Nutrient-Limitation Signaling that Leads to the Transcriptional Induction of Autophagy.

    Current Biology 25(5):546 (2015) PMID 25660547 PMCID PMC4348152

    Autophagy is a conserved process mediating vacuolar degradation and recycling. Autophagy is highly upregulated upon various stresses and is essential for cell survival in deleterious conditions. Autophagy defects are associated with severe pathologies, whereas unchecked autophagy activity causes...
  4. Human trophoblasts confer resistance to viruses implicated in perinatal infection.

    American Journal of Obstetrics and Gynecology 212(1):71.e1 (2015) PMID 25108145 PMCID PMC4275367

    Primary human trophoblasts were previously shown to be resistant to viral infection, and able to confer this resistance to nontrophoblast cells. Can trophoblasts protect nontrophoblastic cells from infection by viruses or other intracellular pathogens that are implicated in perinatal infection? ...
  5. BPIFB3 regulates autophagy and coxsackievirus B replication through a noncanonical pathway independent of the core initiation machinery.

    mBio 5(6):e02147 (2014) PMID 25491355 PMCID PMC4324245

    Enteroviruses require autophagy to facilitate the formation of autophagosome (AP)-like double-membrane vesicles that provide the scaffolding for RNA replication. Here, we identify bactericidal/permeability-increasing protein (BPI) fold-containing family B, member 3 (BPIFB3) as a gene whose silen...
  6. Autophagy as a mechanism of antiviral defense at the maternal-fetal interface.

    Autophagy 9(12):2173 (2013) PMID 24231730

    Mechanisms to protect against viral infections are crucial during pregnancy as maternal-fetal transmission can have serious pathological outcomes, including fetal infection and its sequelae, such as growth restriction, birth defects, and/or fetal death. The trophoblast forms the interface betwee...
  7. Autophagy as a mechanism of antiviral defense at the maternal-fetal interface.

    Autophagy 9(12):2173 (2013) PMID 24231730

    Mechanisms to protect against viral infections are crucial during pregnancy as maternal-fetal transmission can have serious pathological outcomes, including fetal infection and its sequelae, such as growth restriction, birth defects, and/or fetal death. The trophoblast forms the interface betwee...
  8. Lipid raft- and SRC family kinase-dependent entry of coxsackievirus B into human placental trophoblasts.

    Journal of Virology 87(15):8569 (2013) PMID 23720726 PMCID PMC3719791

    Maternal-fetal transmission of group B coxsackieviruses (CVB) during pregnancy has been associated with a number of diverse pathological outcomes, including hydrops fetalis, fetal myocarditis, meningoencephalitis, neurodevelopmental delays, congenital skin lesions, miscarriage, and/or stillbirth...
  9. Lipid raft- and SRC family kinase-dependent entry of coxsackievirus B into human placental trophoblasts.

    Journal of Virology 87(15):8569 (2013) PMID 23720726 PMCID PMC3719791

    Maternal-fetal transmission of group B coxsackieviruses (CVB) during pregnancy has been associated with a number of diverse pathological outcomes, including hydrops fetalis, fetal myocarditis, meningoencephalitis, neurodevelopmental delays, congenital skin lesions, miscarriage, and/or stillbirth...
  10. Human placental trophoblasts confer viral resistance to recipient cells.

    PNAS 110(29):12048 (2013) PMID 23818581 PMCID PMC3718097

    Placental trophoblasts form the interface between the fetal and maternal environments and serve to limit the maternal-fetal spread of viruses. Here we show that cultured primary human placental trophoblasts are highly resistant to infection by a number of viruses and, importantly, confer this re...
  11. Human placental trophoblasts confer viral resistance to recipient cells.

    PNAS 110(29):12048 (2013) PMID 23818581 PMCID PMC3718097

    Placental trophoblasts form the interface between the fetal and maternal environments and serve to limit the maternal-fetal spread of viruses. Here we show that cultured primary human placental trophoblasts are highly resistant to infection by a number of viruses and, importantly, confer this re...
  12. Focal Adhesion Kinase Is a Component of Antiviral RIG-I-like Receptor Signaling

    Cell Host & Microbe 11(2):153 (2012)

    Viruses modulate the actin cytoskeleton at almost every step of their cellular journey from entry to egress. Cellular sensing of these cytoskeletal changes may function in the recognition of viral infection. Here we show that focal adhesion kinase (FAK), a focal adhesion localized tyro...
  13. Focal adhesion kinase is a component of antiviral RIG-I-like receptor signaling.

    Cell Host & Microbe 11(2):153 (2012) PMID 22341464 PMCID PMC3995454

    Viruses modulate the actin cytoskeleton at almost every step of their cellular journey from entry to egress. Cellular sensing of these cytoskeletal changes may function in the recognition of viral infection. Here we show that focal adhesion kinase (FAK), a focal adhesion localized tyrosine kinas...
  14. The actin cytoskeleton as a barrier to virus infection of polarized epithelial cells.

    Viruses 3(12):2462 (2011) PMID 22355449 PMCID PMC3280511

    Many diverse viruses target a polarized epithelial monolayer during host invasion. The polarized epithelium is adept at restricting the movement of solutes, ions, macromolecules, and pathogens across the mucosa. This regulation can be attributed to the presence of a junctional complex between ad...
  15. The coxsackievirus B 3C protease cleaves MAVS and TRIF to attenuate host type I interferon and apoptotic signaling.

    PLoS Pathogens 7(3):e1001311 (2011) PMID 21436888 PMCID PMC3059221

    The host innate immune response to viral infections often involves the activation of parallel pattern recognition receptor (PRR) pathways that converge on the induction of type I interferons (IFNs). Several viruses have evolved sophisticated mechanisms to attenuate antiviral host signaling by di...