1. Ultrasensitive Detection of Ricin Toxin in Multiple Sample Matrixes Using Single-Domain Antibodies.

    Analytical chemistry 87(13):6570 (2015) PMID 26001138

    Ricin is an extremely potent ribosomal inactivating protein listed as a Category B select agent. Although ricin intoxication is not transmittable from person to person, even a single ricin molecule can lead to cell necrosis because it inactivates 1500 ribosomes/min. Since there is currently no v...
  2. The Scope of Analytical Chemistry.

    Analytical chemistry 87(13):6425 (2015) PMID 26086069

  3. Single-molecule arrays for ultrasensitive detection of host immune response to dengue virus infection.

    Journal of Clinical Microbiology 53(5):1722 (2015) PMID 25694521 PMCID PMC4400757

    Dengue virus serotype 2-positive plasma was applied to two indirect single-molecule arrays (Simoas) for the detection of anti-dengue virus IgG and IgM. The Simoas were 1,000 and 10,000 times more sensitive than enzyme-linked immunosorbent assays (ELISAs) for the detection of IgG and IgM, respect...
  4. Stoichiometry of the α-Complementation Reaction of Escherichia coli β-Galactosidase As Revealed through Single-Molecule Studies.

    Biochemistry (Washington) 54(8):1583 (2015) PMID 25668156

    The α-complementation reaction of β-galactosidase was studied at single-molecule resolution using arrays of femtoliter-sized wells. Single molecules of the complementation species were observed to be stable for long periods of time, demonstrating that the α-complementation reaction is irreversib...
  5. Stoichiometry of the α-complementation reaction of Escherichia coli β-galactosidase as revealed through single-molecule studies.

    Biochemistry (Washington) 54(8):1583 (2015) PMID 25668156

    The α-complementation reaction of β-galactosidase was studied at single-molecule resolution using arrays of femtoliter-sized wells. Single molecules of the complementation species were observed to be stable for long periods of time, demonstrating that the α-complementation reaction is irreversib...
  6. Salivary diagnostics using a portable point-of-service platform: a review.

    Clinical Therapeutics 37(3):498 (2015) PMID 25732629 PMCID PMC4390544

    Clinical diagnostics can be improved by faster and more accessible disease detection. Our laboratory has developed a point-of-service (POS) device capable of rapid, sensitive, automated, and multiplexed biomarker detection that uses human saliva instead of other biofluids. Here, we review the te...
  7. Catalytic kinetics of single gold nanoparticles observed via optical microwell arrays.

    Nanotechnology 26(5):055704 (2015) PMID 25590419

    Catalytic activities and kinetics are measured at the single-particle level for gold nanoparticles catalyzing a fluorogenic oxidation reaction. This measurement is accomplished by confining the reactions in optically addressable microwell arrays. Citrate-capped gold nanoparticles are isolated in...
  8. Catalytic kinetics of single gold nanoparticles observed via optical microwell arrays.

    Nanotechnology 26(5):055704 (2015) PMID 25590419

    Catalytic activities and kinetics are measured at the single-particle level for gold nanoparticles catalyzing a fluorogenic oxidation reaction. This measurement is accomplished by confining the reactions in optically addressable microwell arrays. Citrate-capped gold nanoparticles are isolated in...
  9. Catalytic kinetics of single gold nanoparticles observed via optical microwell arrays.

    Nanotechnology 26(5):055704 (2015) PMID 25590419

    Catalytic activities and kinetics are measured at the single-particle level for gold nanoparticles catalyzing a fluorogenic oxidation reaction. This measurement is accomplished by confining the reactions in optically addressable microwell arrays. Citrate-capped gold nanoparticles are isolated in...
  10. Ultra-sensitive protein detection via Single Molecule Arrays towards early stage cancer monitoring.

    Scientific reports 5:11034 (2015) PMID 26052106 PMCID PMC4458912

    The early diagnosis of cancers and continued monitoring of tumor growth would be greatly facilitated by the development of a blood-based, non-invasive, screening technique for early cancer detection. Current technologies for cancer screening and detection typically rely on imaging techniques or ...
  11. Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering.

    Nature Nanotechnology 9(12):969 (2014) PMID 25466541 PMCID PMC4472305

    Rapid progress in identifying disease biomarkers has increased the importance of creating high-performance detection technologies. Over the last decade, the design of many detection platforms has focused on either the nano or micro length scale. Here, we review recent strategies that combine nan...
  12. Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering.

    Nature Nanotechnology 9(12):969 (2014) PMID 25466541

    Rapid progress in identifying disease biomarkers has increased the importance of creating high-performance detection technologies. Over the last decade, the design of many detection platforms has focused on either the nano or micro length scale. Here, we review recent strategies that combine nan...
  13. Disease detection by ultrasensitive quantification of microdosed synthetic urinary biomarkers.

    Journal of the American Chemical Society 136(39):13709 (2014) PMID 25198059 PMCID PMC4183649

    The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting...
  14. Disease detection by ultrasensitive quantification of microdosed synthetic urinary biomarkers.

    Journal of the American Chemical Society 136(39):13709 (2014) PMID 25198059 PMCID PMC4183649

    The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting...
  15. Protein measurements in microwells.

    Lab On a Chip 14(17):3195 (2014) PMID 24825470

    Protein measurements are essential to many fields ranging from fundamental biochemistry to clinical diagnostics. The ability to make measurements of proteins with ultra-sensitivity will enable early diagnosis of diseases by accessing a concentration regime below the detection limit of present pr...
  16. Protein measurements in microwells.

    Lab On a Chip 14(17):3195 (2014) PMID 24825470

    Protein measurements are essential to many fields ranging from fundamental biochemistry to clinical diagnostics. The ability to make measurements of proteins with ultra-sensitivity will enable early diagnosis of diseases by accessing a concentration regime below the detection limit of present pr...
  17. An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.

    Lab On a Chip 14(6):1087 (2014) PMID 24448498 PMCID PMC3996899

    During the last decade, saliva has emerged as a potentially ideal diagnostic biofluid for noninvasive testing. In this paper, we present an automated, integrated platform useable by minimally trained personnel in the field for the diagnosis of respiratory diseases using human saliva as a sample ...
  18. An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.

    Lab On a Chip 14(6):1087 (2014) PMID 24448498 PMCID PMC3996899

    During the last decade, saliva has emerged as a potentially ideal diagnostic biofluid for noninvasive testing. In this paper, we present an automated, integrated platform useable by minimally trained personnel in the field for the diagnosis of respiratory diseases using human saliva as a sample ...
  19. An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.

    Lab On a Chip 14(6):1087 (2014) PMID 24448498 PMCID PMC3996899

    During the last decade, saliva has emerged as a potentially ideal diagnostic biofluid for noninvasive testing. In this paper, we present an automated, integrated platform useable by minimally trained personnel in the field for the diagnosis of respiratory diseases using human saliva as a sample ...
  20. An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.

    Lab On a Chip 14(6):1087 (2014) PMID 24448498 PMCID PMC3996899

    During the last decade, saliva has emerged as a potentially ideal diagnostic biofluid for noninvasive testing. In this paper, we present an automated, integrated platform useable by minimally trained personnel in the field for the diagnosis of respiratory diseases using human saliva as a sample ...