1. Periodic retinoic acid-STRA8 signaling intersects with periodic germ-cell competencies to regulate spermatogenesis.

    PNAS 112(18):E2347 (2015) PMID 25902548 PMCID PMC4426408

    Mammalian spermatogenesis--the transformation of stem cells into millions of haploid spermatozoa--is elaborately organized in time and space. We explored the underlying regulatory mechanisms by genetically and chemically perturbing spermatogenesis in vivo, focusing on spermatogonial differentiat...
  2. Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling.

    PLoS Genetics 11(3):e1005019 (2015) PMID 25739037 PMCID PMC4349450

    In mouse embryos at mid-gestation, primordial germ cells (PGCs) undergo licensing to become gametogenesis-competent cells (GCCs), gaining the capacity for meiotic initiation and sexual differentiation. GCCs then initiate either oogenesis or spermatogenesis in response to gonadal cues. Germ cell ...
  3. Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling.

    PLoS Genetics 11(3):e1005019 (2015) PMID 25739037

    In mouse embryos at mid-gestation, primordial germ cells (PGCs) undergo licensing to become gametogenesis-competent cells (GCCs), gaining the capacity for meiotic initiation and sexual differentiation. GCCs then initiate either oogenesis or spermatogenesis in response to gonadal cues. Germ cell ...
  4. Sex chromosome-to-autosome transposition events counter Y-chromosome gene loss in mammals.

    Genome biology 16(1):104 (2015) PMID 26017895 PMCID PMC4446799

    Although the mammalian X and Y chromosomes evolved from a single pair of autosomes, they are highly differentiated: the Y chromosome is dramatically smaller than the X and has lost most of its genes. The surviving genes are a specialized set with extraordinary evolutionary longevity. Most mammal...
  5. The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.

    Journal of Biological Chemistry 289(50):34490 (2014) PMID 25320092 PMCID PMC4263858

    ER-resident proteins destined for degradation are dislocated into the cytosol by components of the ER quality control machinery for proteasomal degradation. Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating/E3 ligase complexes. UBE2J1 is one of the well-characte...
  6. The E2 Ubiquitin-conjugating Enzyme UBE2J1 Is Required for Spermiogenesis in Mice.

    Journal of Biological Chemistry 289(50):34490 (2014) PMID 25320092 PMCID PMC4263858

    ER-resident proteins destined for degradation are dislocated into the cytosol by components of the ER quality control machinery for proteasomal degradation. Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating/E3 ligase complexes. UBE2J1 is one of the well-characte...
  7. The E2 Ubiquitin-conjugating Enzyme UBE2J1 Is Required for Spermiogenesis in Mice.

    Journal of Biological Chemistry 289(50):34490 (2014) PMID 25320092 PMCID PMC4263858

    ER-resident proteins destined for degradation are dislocated into the cytosol by components of the ER quality control machinery for proteasomal degradation. Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating/E3 ligase complexes. UBE2J1 is one of the well-characte...
  8. The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.

    Journal of Biological Chemistry 289(50):34490 (2014) PMID 25320092 PMCID PMC4263858

    ER-resident proteins destined for degradation are dislocated into the cytosol by components of the ER quality control machinery for proteasomal degradation. Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating/E3 ligase complexes. UBE2J1 is one of the well-characte...
  9. The E2 Ubiquitin-conjugating Enzyme UBE2J1 Is Required for Spermiogenesis in Mice.

    Journal of Biological Chemistry 289(50):34490 (2014) PMID 25320092

    ER-resident proteins destined for degradation are dislocated into the cytosol by components of the ER quality control machinery for proteasomal degradation. Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating/E3 ligase complexes. UBE2J1 is one of the well-characte...
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  10. Excess cholesterol induces mouse egg activation and may cause female infertility.

    PNAS 111(46):E4972 (2014) PMID 25368174 PMCID PMC4246315

    The HDL receptor scavenger receptor, class B type I (SR-BI) controls the structure and fate of plasma HDL. Female SR-BI KO mice are infertile, apparently because of their abnormal cholesterol-enriched HDL particles. We examined the growth and meiotic progression of SR-BI KO oocytes and found tha...
  11. Excess cholesterol induces mouse egg activation and may cause female infertility.

    PNAS 111(46):E4972 (2014) PMID 25368174 PMCID PMC4246315

    The HDL receptor scavenger receptor, class B type I (SR-BI) controls the structure and fate of plasma HDL. Female SR-BI KO mice are infertile, apparently because of their abnormal cholesterol-enriched HDL particles. We examined the growth and meiotic progression of SR-BI KO oocytes and found tha...
  12. Excess cholesterol induces mouse egg activation and may cause female infertility.

    PNAS 111(46):E4972 (2014) PMID 25368174 PMCID PMC4246315

    The HDL receptor scavenger receptor, class B type I (SR-BI) controls the structure and fate of plasma HDL. Female SR-BI KO mice are infertile, apparently because of their abnormal cholesterol-enriched HDL particles. We examined the growth and meiotic progression of SR-BI KO oocytes and found tha...
  13. Sequencing the mouse y chromosome reveals convergent gene acquisition and amplification on both sex chromosomes.

    Cell 159(4):800 (2014) PMID 25417157 PMCID PMC4260969

    We sequenced the MSY (male-specific region of the Y chromosome) of the C57BL/6J strain of the laboratory mouse Mus musculus. In contrast to theories that Y chromosomes are heterochromatic and gene poor, the mouse MSY is 99.9% euchromatic and contains about 700 protein-coding genes. Only 2% of th...
  14. Sequencing the mouse Y chromosome reveals convergent gene acquisition and amplification on both sex chromosomes.

    Cell 159(4):800 (2014) PMID 25417157 PMCID PMC4260969

    We sequenced the MSY (male-specific region of the Y chromosome) of the C57BL/6J strain of the laboratory mouse Mus musculus. In contrast to theories that Y chromosomes are heterochromatic and gene poor, the mouse MSY is 99.9% euchromatic and contains about 700 protein-coding genes. Only 2% of th...
  15. Poised chromatin in the mammalian germ line.

    Development 141(19):3619 (2014) PMID 25249456 PMCID PMC4197577

    Poised (bivalent) chromatin is defined by the simultaneous presence of histone modifications associated with both gene activation and repression. This epigenetic feature was first observed at promoters of lineage-specific regulatory genes in embryonic stem cells in culture. More recent work has ...
  16. Poised chromatin in the mammalian germ line.

    Development 141(19):3619 (2014) PMID 25249456 PMCID PMC4197577

    Poised (bivalent) chromatin is defined by the simultaneous presence of histone modifications associated with both gene activation and repression. This epigenetic feature was first observed at promoters of lineage-specific regulatory genes in embryonic stem cells in culture. More recent work has ...
  17. Retinoic acid activates two pathways required for meiosis in mice.

    PLoS Genetics 10(8):e1004541 (2014) PMID 25102060 PMCID PMC4125102

    In all sexually reproducing organisms, cells of the germ line must transition from mitosis to meiosis. In mice, retinoic acid (RA), the extrinsic signal for meiotic initiation, activates transcription of Stra8, which is required for meiotic DNA replication and the subsequent processes of meiotic...
  18. Retinoic acid activates two pathways required for meiosis in mice.

    PLoS Genetics 10(8):e1004541 (2014) PMID 25102060 PMCID PMC4125102

    In all sexually reproducing organisms, cells of the germ line must transition from mitosis to meiosis. In mice, retinoic acid (RA), the extrinsic signal for meiotic initiation, activates transcription of Stra8, which is required for meiotic DNA replication and the subsequent processes of meiotic...
  19. A chromatin-dependent role of the fragile X mental retardation protein FMRP in the DNA damage response.

    Cell 157(4):869 (2014) PMID 24813610 PMCID PMC4038154

    Fragile X syndrome, a common form of inherited intellectual disability, is caused by loss of the fragile X mental retardation protein FMRP. FMRP is present predominantly in the cytoplasm, where it regulates translation of proteins that are important for synaptic function. We identify FMRP as a c...
  20. Mammalian Y chromosomes retain widely expressed dosage-sensitive regulators.

    Nature 508(7497):494 (2014) PMID 24759411 PMCID PMC4139287

    The human X and Y chromosomes evolved from an ordinary pair of autosomes, but millions of years ago genetic decay ravaged the Y chromosome, and only three per cent of its ancestral genes survived. We reconstructed the evolution of the Y chromosome across eight mammals to identify biases in gene ...