1. …But is it an improvement?

    Biochemistry and Molecular Biology Education 43(4):301 (2015) PMID 26173430

  2. The symphony of autophagy and calcium signaling.

    Autophagy 11(7):973 (2015) PMID 26046237

    Posttranslational regulation of macroautophagy (hereafter autophagy), including phosphorylating and dephosphorylating components of the autophagy-related (Atg) core machinery and the corresponding upstream transcriptional factors, is important for the precise modulation of autophagy levels. Seve...
  3. A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy.

    Nature Cell Biology 17(7):930 (2015) PMID 26098573

    Autophagy is an essential eukaryotic pathway requiring tight regulation to maintain homeostasis and preclude disease. Using yeast and mammalian cells, we report a conserved mechanism of autophagy regulation by RNA helicase RCK family members in association with the decapping enzyme Dcp2. Under n...
  4. Molecular interactions of the Saccharomyces cerevisiae Atg1 complex provide insights into assembly and regulatory mechanisms.

    Autophagy 11(6):891 (2015) PMID 25998554 PMCID PMC4502708

    The Atg1 complex, which contains 5 major subunits: Atg1, Atg13, Atg17, Atg29, and Atg31, regulates the induction of autophagy and autophagosome formation. To gain a better understanding of the overall architecture and assembly mechanism of this essential autophagy regulatory complex, we have rec...
  5. TOS-sing aside the glycolytic role of HK2/hexokinase-II to activate autophagy.

    Autophagy 11(6):865 (2015) PMID 26075875 PMCID PMC4502652

    Hexokinase is the first enzyme in the glycolytic pathway catalyzing the reaction in which glucose is phosphorylated into glucose-6-phosphate. Mammals possess 4 isoforms of hexokinase; HK2 (hexokinase 2) is the predominant form in insulin-sensitive tissues such as adipocytes, as well as skeletal ...
  6. Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium.

    Autophagy 11(6):939 (2015) PMID 26075877 PMCID PMC4502815

    Autophagy regulates cellular homeostasis and response to environmental stress. Within the retinal pigment epithelium (RPE) of the eye, the level of autophagy can change with both age and disease. The purpose of this study is to determine the relationship between reduced autophagy and age-related...
  7. How to control self-digestion: transcriptional, post-transcriptional, and post-translational regulation of autophagy.

    Trends in Cell Biology 25(6):354 (2015) PMID 25759175 PMCID PMC4441840

    Macroautophagy (hereafter autophagy), literally defined as a type of self-eating, is a dynamic cellular process in which cytoplasm is sequestered within a unique compartment termed the phagophore. Upon completion, the phagophore matures into a double-membrane autophagosome that fuses with the ly...
  8. Not learning how to ride a bike: The lecture approach.

    Biochemistry and Molecular Biology Education 43(3):210 (2015) PMID 25787694

  9. Autophagy: research topic, painting, poem, dance…: The combination of art and information can enhance the enjoyment and effectiveness of learning.

    EMBO Reports 16(5):547 (2015) PMID 25838538 PMCID PMC4428040

  10. Rph1 mediates the nutrient-limitation signaling pathway leading to transcriptional activation of autophagy.

    Autophagy 11(4):718 (2015) PMID 25751780

    To maintain proper cellular homeostasis, the magnitude of autophagy activity has to be finely tuned in response to environmental changes. Many aspects of autophagy regulation have been extensively studied: pathways integrating signals through the master regulators TORC1 and PKA lead to multiple ...
  11. Toward an understanding of autophagosome-lysosome fusion: The unsuspected role of ATG14.

    Autophagy 11(4):583 (2015) PMID 25920502

    Although largely overlooked relative to the process of phagophore formation, the mechanism through which autophagosomes fuse with lysosomes is a critical aspect of macroautophagy that is not fully understood. In particular, this step must be carefully regulated to prevent premature fusion of an ...
  12. The yeast Saccharomyces cerevisiae: An overview of methods to study autophagy progression

    Methods 75:3 (2015) PMID 25526918

    Macroautophagy (hereafter autophagy) is a highly evolutionarily conserved process essential for sustaining cellular integrity, homeostasis, and survival. Most eukaryotic cells constitutively undergo autophagy at a low basal level. However, various stimuli, including starvation, organel...
  13. Assays for the biochemical and ultrastructural measurement of selective and nonselective types of autophagy in the yeast Saccharomyces cerevisiae

    Methods 75:141 (2015) PMID 25484341

    Autophagy is a conserved intracellular catabolic pathway that degrades unnecessary or dysfunctional cellular components. Components destined for degradation are sequestered into double-membrane vesicles called autophagosomes, which subsequently fuse with the vacuole/lysosome delivering...
  14. Rph1/KDM4 Mediates Nutrient-Limitation Signaling that Leads to the Transcriptional Induction of Autophagy.

    Current Biology 25(5):546 (2015) PMID 25660547 PMCID PMC4348152

    Autophagy is a conserved process mediating vacuolar degradation and recycling. Autophagy is highly upregulated upon various stresses and is essential for cell survival in deleterious conditions. Autophagy defects are associated with severe pathologies, whereas unchecked autophagy activity causes...
  15. The yeast Saccharomyces cerevisiae: An overview of methods to study autophagy progression.

    Methods 75:3 (2015) PMID 25526918 PMCID PMC4355233

    Macroautophagy (hereafter autophagy) is a highly evolutionarily conserved process essential for sustaining cellular integrity, homeostasis, and survival. Most eukaryotic cells constitutively undergo autophagy at a low basal level. However, various stimuli, including starvation, organelle deterio...
  16. Atg23 and Atg27 Act at the Early Stages of Atg9 Trafficking in S. cerevisiae.

    Traffic 16(2):172 (2015) PMID 25385507 PMCID PMC4305007

    Atg9 is a conserved multipass transmembrane protein with an essential role in autophagy. In Saccharomyces cerevisiae, it travels through the secretory pathway to a unique compartment, the Atg9 peripheral structures. These structures are then targeted to the phagophore assembly site (PAS), where ...
  17. Atg23 and Atg27 act at the early stages of Atg9 trafficking in S. cerevisiae.

    Traffic 16(2):172 (2015) PMID 25385507 PMCID PMC4305007

    Atg9 is a conserved multipass transmembrane protein with an essential role in autophagy. In Saccharomyces cerevisiae, it travels through the secretory pathway to a unique compartment, the Atg9 peripheral structures. These structures are then targeted to the phagophore assembly site (PAS), where ...
  18. Molecular mechanisms of autophagy in the cardiovascular system.

    Circulation Research 116(3):456 (2015) PMID 25634969 PMCID PMC4313620

    Autophagy is a catabolic recycling pathway triggered by various intra- or extracellular stimuli that is conserved from yeast to mammals. During autophagy, diverse cytosolic constituents are enveloped by double-membrane vesicles, autophagosomes, which later fuse with lysosomes or the vacuole to d...
  19. Posttranslational modification of autophagy-related proteins in macroautophagy.

    Autophagy 11(1):28 (2015) PMID 25484070

    Macroautophagy is an intracellular catabolic process involved in the formation of multiple membrane structures ranging from phagophores to autophagosomes and autolysosomes. Dysfunction of macroautophagy is implicated in both physiological and pathological conditions. To date, 38 autophagy-relate...
  20. A few key points about figure presentation.

    Autophagy 11(1):1 (2015) PMID 25607738