1. X-ray structure of the arenavirus glycoprotein GP2 in its postfusion hairpin conformation.

    PNAS 108(50):19967 (2011) PMID 22123988 PMCID PMC3250147

    Arenaviruses are important agents of zoonotic disease worldwide. The virions expose a tripartite envelope glycoprotein complex at their surface, formed by the glycoprotein subunits GP1, GP2 and the stable signal peptide. This complex is responsible for binding to target cells and for the subsequ...
  2. X-ray structure of the arenavirus glycoprotein GP2 in its postfusion hairpin conformation.

    PNAS 108(50):19967 (2011) PMID 22123988 PMCID PMC3250147

    Arenaviruses are important agents of zoonotic disease worldwide. The virions expose a tripartite envelope glycoprotein complex at their surface, formed by the glycoprotein subunits GP1, GP2 and the stable signal peptide. This complex is responsible for binding to target cells and for the subsequ...
  3. Impaired antibody response causes persistence of prototypic T cell-contained virus.

    PLoS Biology 7(4):e1000080 (2009) PMID 19355789 PMCID PMC2672599

    CD8 T cells are recognized key players in control of persistent virus infections, but increasing evidence suggests that assistance from other immune mediators is also needed. Here, we investigated whether specific antibody responses contribute to control of lymphocytic choriomeningitis virus (LC...
  4. Impaired antibody response causes persistence of prototypic T cell-contained virus.

    PLoS Biology 7(4):e1000080 (2009) PMID 19355789 PMCID PMC2672599

    CD8 T cells are recognized key players in control of persistent virus infections, but increasing evidence suggests that assistance from other immune mediators is also needed. Here, we investigated whether specific antibody responses contribute to control of lymphocytic choriomeningitis virus (LC...
  5. Lymphocytic choriomeningitis virus uses a novel endocytic pathway for infectious entry via late endosomes

    Virology 378(1):21 (2008) PMID 18554681

    The endocytic entry of lymphocytic choriomeningitis virus (LCMV) into host cells was compared to the entry of viruses known to exploit clathrin or caveolae/raft-dependent pathways. Pharmacological inhibitors, expression of pathway-specific dominant-negative constructs, and siRNA silencing o...
  6. Lymphocytic choriomeningitis virus uses a novel endocytic pathway for infectious entry via late endosomes

    Virology 378(1):21 (2008) PMID 18554681

    The endocytic entry of lymphocytic choriomeningitis virus (LCMV) into host cells was compared to the entry of viruses known to exploit clathrin or caveolae/raft-dependent pathways. Pharmacological inhibitors, expression of pathway-specific dominant-negative constructs, and siRNA silencing o...
  7. Lymphocytic choriomeningitis virus uses a novel endocytic pathway for infectious entry via late endosomes

    Virology 378(1):21 (2008) PMID 18554681

    The endocytic entry of lymphocytic choriomeningitis virus (LCMV) into host cells was compared to the entry of viruses known to exploit clathrin or caveolae/raft-dependent pathways. Pharmacological inhibitors, expression of pathway-specific dominant-negative constructs, and siRNA silencing o...
  8. Extralymphatic virus sanctuaries as a consequence of potent T-cell activation.

    Nature Medicine 13(11):1316 (2007) PMID 17982463

    T helper cells can support the functions of CD8(+) T cells against persistently infecting viruses such as murine lymphocytic choriomeningitis virus (LCMV), cytomegalovirus, hepatitis C virus and HIV. These viruses often resist complete elimination and remain detectable at sanctuary sites, such a...
  9. Early antibodies specific for the neutralizing epitope on the receptor binding subunit of the lymphocytic choriomeningitis virus glycoprotein fail to neutralize the virus.

    Journal of Virology 81(21):11650 (2007) PMID 17699567 PMCID PMC2168768

    Lymphocytic choriomeningitis virus (LCMV) is a murine arenavirus whose glycoprotein consists of a transmembrane subunit (GP-2) and a receptor-binding subunit (GP-1). LCMV-neutralizing antibodies (nAbs) are directed against a single site on GP-1 and occur 1 month after the infection of cytotoxic-...
  10. Early antibodies specific for the neutralizing epitope on the receptor binding subunit of the lymphocytic choriomeningitis virus glycoprotein fail to neutralize the virus.

    Journal of Virology 81(21):11650 (2007) PMID 17699567 PMCID PMC2168768

    Lymphocytic choriomeningitis virus (LCMV) is a murine arenavirus whose glycoprotein consists of a transmembrane subunit (GP-2) and a receptor-binding subunit (GP-1). LCMV-neutralizing antibodies (nAbs) are directed against a single site on GP-1 and occur 1 month after the infection of cytotoxic-...
  11. Extralymphatic virus sanctuaries as a consequence of potent T-cell activation.

    Nature Medicine 13(11):1316 (2007) PMID 17982463

    T helper cells can support the functions of CD8(+) T cells against persistently infecting viruses such as murine lymphocytic choriomeningitis virus (LCMV), cytomegalovirus, hepatitis C virus and HIV. These viruses often resist complete elimination and remain detectable at sanctuary sites, such a...
  12. A lymphocytic choriomeningitis virus glycoprotein variant that is retained in the endoplasmic reticulum efficiently cross-primes CD8(+) T cell responses.

    PNAS 104(33):13426 (2007) PMID 17686978 PMCID PMC1948914

    Recent studies indicate that T cell cross-priming preferentially occurs against long-lived, stable proteins. We have studied cross-priming by using the glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV), a protein that normally is not MHC class I cross-presented. This study shows tha...
  13. A lymphocytic choriomeningitis virus glycoprotein variant that is retained in the endoplasmic reticulum efficiently cross-primes CD8(+) T cell responses.

    PNAS 104(33):13426 (2007) PMID 17686978 PMCID PMC1948914

    Recent studies indicate that T cell cross-priming preferentially occurs against long-lived, stable proteins. We have studied cross-priming by using the glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV), a protein that normally is not MHC class I cross-presented. This study shows tha...
  14. Decreased tumor surveillance after adoptive T-cell therapy.

    Cancer Research 67(15):7467 (2007) PMID 17671217

    The effect of cancer immunotherapy on the endogenous immune response against tumors is largely unknown. Therefore, we studied immune responses against murine tumors expressing the glycoprotein (GP) and/or nucleoprotein of lymphocytic choriomeningitis virus (LCMV) with or without adoptive T-cell ...
  15. Decreased tumor surveillance after adoptive T-cell therapy.

    Cancer Research 67(15):7467 (2007) PMID 17671217

    The effect of cancer immunotherapy on the endogenous immune response against tumors is largely unknown. Therefore, we studied immune responses against murine tumors expressing the glycoprotein (GP) and/or nucleoprotein of lymphocytic choriomeningitis virus (LCMV) with or without adoptive T-cell ...
  16. "Negative vaccination" by specific CD4 T cell tolerisation enhances virus-specific protective antibody responses.

    PLoS ONE 2(11):e1162 (2007) PMID 18000535 PMCID PMC2048666

    Cooperation of CD4+ T helper cells with specific B cells is crucial for protective vaccination against pathogens by inducing long-lived neutralizing antibody responses. During infection with persistence-prone viruses, prolonged virus replication correlates with low neutralizing antibody response...
  17. "Negative vaccination" by specific CD4 T cell tolerisation enhances virus-specific protective antibody responses.

    PLoS ONE 2(11):e1162 (2007) PMID 18000535 PMCID PMC2048666

    Cooperation of CD4+ T helper cells with specific B cells is crucial for protective vaccination against pathogens by inducing long-lived neutralizing antibody responses. During infection with persistence-prone viruses, prolonged virus replication correlates with low neutralizing antibody response...
  18. Functional CD8+ but not CD4+ T cell responses develop independent of thymic epithelial MHC.

    PNAS 103(39):14435 (2006) PMID 16983067 PMCID PMC1599980

    The role of nonthymic epithelial (non-TE) MHC in T cell repertoire selection remains controversial. To analyze the relative roles of thymic epithelial (TE) and non-TE MHC in T cell repertoire selection, we have generated tetraparental aggregation chimeras (B6-nude<=>BALB/c and B6<=>BALB/c-nude) ...
  19. Functional CD8+ but not CD4+ T cell responses develop independent of thymic epithelial MHC.

    PNAS 103(39):14435 (2006) PMID 16983067 PMCID PMC1599980

    The role of nonthymic epithelial (non-TE) MHC in T cell repertoire selection remains controversial. To analyze the relative roles of thymic epithelial (TE) and non-TE MHC in T cell repertoire selection, we have generated tetraparental aggregation chimeras (B6-nude<=>BALB/c and B6<=>BALB/c-nude) ...
  20. Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread.

    Journal of Experimental Medicine 203(8):2033 (2006) PMID 16880253 PMCID PMC2118372

    The biological relevance of nonneutralizing antibodies elicited early after infection with noncytopathic persistence-prone viruses is unclear. We demonstrate that cytotoxic T lymphocyte-deficient TgH(KL25) mice, which are transgenic for the heavy chain of the lymphocytic choriomeningitis virus (...