1. Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase.

    Methods in Molecular Biology 1312:481 (2015) PMID 26044031

    Dactyloscopy or fingerprint identification is a vital part of forensic evidence. Identification with fingerprints has been known since the finding of finger impressions on the clay surface of Babylonian legal contracts almost 4,000 years ago. The skin on the fingers and palms appears as grooves ...
  2. On-Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions.

    Methods in Molecular Biology 1314:255 (2015) PMID 26139273

    Calcium plays an important role in many biochemical processes in different cell types. This divalent cation interacts with specific calcium-binding proteins that serve as calcium sensors and regulatory proteins to mediate its function. Previously, we found that calcium was involved in the protei...
  3. Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane.

    Methods in Molecular Biology 1314:273 (2015) PMID 26139275

    Minor impurities in tryptic peptide digests can affect the signal obtained in matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Therefore, it becomes necessary to purify the digests, especially those that fail to yield good mass spectra. Here, we describe a simple pro...
  4. Invisible Ink Marking in ECL Membrane Assays.

    Methods in Molecular Biology 1314:375 (2015) PMID 26139285

    Invisible ink and writing secret messages have been part of man's fantasy, having proven useful in clandestine and high sensitivity areas. Security inks, made up of invisible materials that give printed, anti-photocopy images capable of being read only under special environments, have become imp...
  5. Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel.

    Methods in Molecular Biology 1312:77 (2015) PMID 26043992

    Western blotting enables the detection and characterization of proteins of low abundance. Sodium dodecyl sulfate (SDS) polyacrylamide gel-separated proteins are normally transferred electrophoretically to nitrocellulose or polyvinylidene difluoride membranes. Here we describe the transfer protei...
  6. Western blotting: an introduction.

    Methods in Molecular Biology 1312:17 (2015) PMID 26043986

    Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membran...
  7. SDS-PAGE to Immunoblot in One Hour.

    Methods in Molecular Biology 1312:449 (2015) PMID 26044026

    An ultra-rapid method for electrophoresing proteins on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transfer of proteins to nitrocellulose membranes, and immunoblotting is described here. Electrophoresis of the autoantigens La and Ro60, as well as molecular weight standards on a 4-...
  8. Other notable protein blotting methods: a brief review.

    Methods in Molecular Biology 1312:487 (2015) PMID 26044032

    Proteins have been transferred from the gel to the membrane by a variety of methods. These include vacuum blotting, centrifuge blotting, electroblotting of proteins to Teflon tape and membranes for N- and C-terminal sequence analysis, multiple tissue blotting, a two-step transfer of low- and hig...
  9. From little helpers to automation.

    Methods in Molecular Biology 1312:31 (2015) PMID 26043987

    Western blot technology has continually evolved to enhance sensitivity, speed, and ease of operation. For enhancing awareness to these developments, this brief review outlines a representative selection of methods and devices, many of which are commercial products. In particular, the steps taken...
  10. Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane.

    Methods in Molecular Biology 1314:165 (2015) PMID 26139264

    Antibodies directed against ribonucleoprotein (RNP) particles are observed in systemic lupus erythematosus. Ro RNP particle is one such target. It is composed of a 60 kDa protein (Ro 60 or SS-A) that is non-covalently associated with at least one of the four short uridine-rich RNAs (the hY RNAs)...
  11. Western blotting of high and low molecular weight proteins using heat.

    Methods in Molecular Biology 1312:247 (2015) PMID 26044007

    A method for the electrophoretic transfer of high and low molecular weight proteins to nitrocellulose membranes following sodium dodecyl sulfate (SDS) polyacrylamide gel is described here. The transfer was performed with heated (70-75 °C) normal transfer buffer from which methanol had been omitt...
  12. Membrane strip affinity purification of autoantibodies.

    Methods in Molecular Biology 1312:257 (2015) PMID 26044008

    A method for affinity purification of autoantibodies from a membrane strip using small volumes of human sera is described. The membrane strip is excised from a western blot containing a target antigen electrophoretically transferred from a sodium dodecyl sulfate (SDS) polyacrylamide gel. This me...
  13. Strip immunoblotting of multiple antigenic peptides.

    Methods in Molecular Biology 1312:269 (2015) PMID 26044009

    Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis can be employed to efficiently separate multiple antigenic peptides (MAPs). Moreover, the electrophoresed MAPs are amenable for transfer to nitrocellulose membrane for immunoblotting. MAPs involve a hepta lysine core with end groups...
  14. Analysis of antibody clonotype by affinity immunoblotting.

    Methods in Molecular Biology 1312:109 (2015) PMID 26043996

    A sensitive and specific method to analyze specific antibody clonotype changes in a lupus patient who developed autoantibodies to the Ro 60 autoantigen under observation is described. Patient sera, collected over several years, were separated by flatbed isoelectric focusing (IEF) and analyzed by...
  15. A miniaturized blotting system for simultaneous detection of different autoantibodies.

    Methods in Molecular Biology 1312:165 (2015) PMID 26044001

    Sera of tumor patients frequently contain autoantibodies to tumor associated antigens. Here we describe a miniaturized immunoblot platform allowing us to screen sera of patients for the presence of autoantibodies to ten autoantigens in parallel.
  16. Haematological manifestations of lupus.

    Lupus Science & Medicine 2(1):e000078 (2015) PMID 25861458 PMCID PMC4378375

    Our purpose was to compile information on the haematological manifestations of systemic lupus erythematosus (SLE), namely leucopenia, lymphopenia, thrombocytopenia, autoimmune haemolytic anaemia (AIHA), thrombotic thrombocytopenic purpura (TTP) and myelofibrosis. During our search of the English...
  17. Other Notable Methods of Membrane Protein Detection: A Brief Review.

    Methods in Molecular Biology 1314:357 (2015) PMID 26139283

    Several techniques have been employed to detect proteins on membranes. These include the use of quantum dot luminescent labels, oxyblot immunochemical detection, polymer immunocomplexes, "coupled" probing approach, in situ renaturation of proteins for detecting enzyme activities in crude or puri...
  18. Antibody-secreting cell specificity in labial salivary glands reflects the clinical presentation and serology in patients with Sjögren's syndrome.

    Arthritis & rheumatology (Hoboken, N.J.) 66(12):3445 (2014) PMID 25199908 PMCID PMC4245382

    The serologic hallmark of primary Sjögren's syndrome (SS) is the presence of IgG antibodies specific for Ro (SSA) and La (SSB). The molecular characteristics of gland-derived B cells at the site of primary SS inflammation have been described previously; however, parallels between glandular antib...
  19. Antibody-secreting cell specificity in labial salivary glands reflects the clinical presentation and serology in patients with Sjögren's syndrome.

    Arthritis & rheumatology (Hoboken, N.J.) 66(12):3445 (2014) PMID 25199908 PMCID PMC4245382

    The serologic hallmark of primary Sjögren's syndrome (SS) is the presence of IgG antibodies specific for Ro (SSA) and La (SSB). The molecular characteristics of gland-derived B cells at the site of primary SS inflammation have been described previously; however, parallels between glandular antib...
  20. Antibody-secreting cell specificity in labial salivary glands reflects the clinical presentation and serology in patients with Sjögren's syndrome.

    Arthritis & rheumatology (Hoboken, N.J.) 66(12):3445 (2014) PMID 25199908 PMCID PMC4245382

    The serologic hallmark of primary Sjögren's syndrome (SS) is the presence of IgG antibodies specific for Ro (SSA) and La (SSB). The molecular characteristics of gland-derived B cells at the site of primary SS inflammation have been described previously; however, parallels between glandular antib...