Autoantibodies in Sjögren's Syndrome.
Rheumatic Disease Clinics of North America 42(3):419 (2016)
We compiled information on antibodies in Sjögren syndrome, focusing more on clinical manifestations associated with anti-Ro/SSA and anti-La/SSB antibodies and studies regarding novel antibodies. We reviewed previous as well as most recent studies with the subject heading Sjogren in combination w...
Klinefelter's syndrome (47,XXY) is in excess among men with Sjögren's syndrome.
Clinical Immunology 168:25 (2016)
Primary Sjögren's syndrome (pSS) has a strong female bias. We evaluated an X chromosome dose effect by analyzing 47,XXY (Klinefelter's syndrome, 1 in 500 live male births) among subjects with pSS. 47,XXY was determined by examination of fluorescence intensity of single nucleotide polymorphisms f...
Previous diagnosis of Sjögren's Syndrome as rheumatoid arthritis or systemic lupus erythematosus.
Rheumatology 55(7):1195 (2016)
The diagnosis of SS is often difficult and many patients are symptomatic for years with other diagnoses before confirmation of SS. Our aim was to determine whether overlapping clinical and serologic features with RA and SLE may in part drive the misdiagnoses.
A total of 1175 sicca patients were ...
X Chromosome Dose and Sex Bias in Autoimmune Diseases: Increased Prevalence of 47,XXX in Systemic Lupus Erythematosus and Sjögren's Syndrome.
Arthritis & rheumatology (Hoboken, N.J.) 68(5):1290 (2016)
More than 80% of autoimmune disease predominantly affects females, but the mechanism for this female bias is poorly understood. We suspected that an X chromosome dose effect accounts for this, and we undertook this study to test our hypothesis that trisomy X (47,XXX; occurring in ∼1 in 1,000 liv...
Letter to the Editor: Getting to the True Values of Thyroglobulin and Anti-Thyroglobulin Antibodies.
Journal of Clinical Endocrinology & Metabolism 101(3):L38 (2016)
Proteomics in rheumatology.
International Journal of Rheumatic Diseases 18(8):815 (2015)
Therapeutic potential of curcumin and curcumin analogues in rheumatology.
International Journal of Rheumatic Diseases 18(6):591 (2015)
Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel.
Methods in Molecular Biology 1312:77 (2015)
Western blotting enables the detection and characterization of proteins of low abundance. Sodium dodecyl sulfate (SDS) polyacrylamide gel-separated proteins are normally transferred electrophoretically to nitrocellulose or polyvinylidene difluoride membranes. Here we describe the transfer protei...
Western blotting of high and low molecular weight proteins using heat.
Methods in Molecular Biology 1312:247 (2015)
A method for the electrophoretic transfer of high and low molecular weight proteins to nitrocellulose membranes following sodium dodecyl sulfate (SDS) polyacrylamide gel is described here. The transfer was performed with heated (70-75 °C) normal transfer buffer from which methanol had been omitt...
Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane.
Methods in Molecular Biology 1314:165 (2015)
Antibodies directed against ribonucleoprotein (RNP) particles are observed in systemic lupus erythematosus. Ro RNP particle is one such target. It is composed of a 60 kDa protein (Ro 60 or SS-A) that is non-covalently associated with at least one of the four short uridine-rich RNAs (the hY RNAs)...
Other Notable Methods of Membrane Protein Detection: A Brief Review.
Methods in Molecular Biology 1314:357 (2015)
Several techniques have been employed to detect proteins on membranes. These include the use of quantum dot luminescent labels, oxyblot immunochemical detection, polymer immunocomplexes, "coupled" probing approach, in situ renaturation of proteins for detecting enzyme activities in crude or puri...
Significantly reduced lymphadenopathy, salivary gland infiltrates and proteinuria in MRL-lpr/lpr mice treated with ultrasoluble curcumin/turmeric: increased survival with curcumin treatment.
Lupus Science & Medicine 2(1):e000114 (2015)
Commercial curcumin (CU), derived from food spice turmeric (TU), has been widely studied as a potential therapeutic for a variety of oncological and inflammatory conditions. Lack of solubility/bioavailability has hindered curcumin's therapeutic efficacy in human diseases. We have solubilised cur...
Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane.
Methods in Molecular Biology 1314:273 (2015)
Minor impurities in tryptic peptide digests can affect the signal obtained in matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Therefore, it becomes necessary to purify the digests, especially those that fail to yield good mass spectra. Here, we describe a simple pro...
From little helpers to automation.
Methods in Molecular Biology 1312:31 (2015)
Western blot technology has continually evolved to enhance sensitivity, speed, and ease of operation. For enhancing awareness to these developments, this brief review outlines a representative selection of methods and devices, many of which are commercial products. In particular, the steps taken...
Other notable protein blotting methods: a brief review.
Methods in Molecular Biology 1312:487 (2015)
Proteins have been transferred from the gel to the membrane by a variety of methods. These include vacuum blotting, centrifuge blotting, electroblotting of proteins to Teflon tape and membranes for N- and C-terminal sequence analysis, multiple tissue blotting, a two-step transfer of low- and hig...
Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase.
Methods in Molecular Biology 1312:481 (2015)
Dactyloscopy or fingerprint identification is a vital part of forensic evidence. Identification with fingerprints has been known since the finding of finger impressions on the clay surface of Babylonian legal contracts almost 4,000 years ago. The skin on the fingers and palms appears as grooves ...
Strip immunoblotting of multiple antigenic peptides.
Methods in Molecular Biology 1312:269 (2015)
Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis can be employed to efficiently separate multiple antigenic peptides (MAPs). Moreover, the electrophoresed MAPs are amenable for transfer to nitrocellulose membrane for immunoblotting. MAPs involve a hepta lysine core with end groups...
Association between Secondary and Primary Sjögren's Syndrome in a Large Collection of Lupus Families.
Autoimmune Diseases 2015:298506 (2015)
Objective. Systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS) share clinical and immunogenetic features and may occur together. We undertook this study to determine the risk of primary SS among SLE-unaffected relatives of SLE patients and whether or not primary and secondary SS tende...
Invisible Ink Marking in ECL Membrane Assays.
Methods in Molecular Biology 1314:375 (2015)
Invisible ink and writing secret messages have been part of man's fantasy, having proven useful in clandestine and high sensitivity areas. Security inks, made up of invisible materials that give printed, anti-photocopy images capable of being read only under special environments, have become imp...
A miniaturized blotting system for simultaneous detection of different autoantibodies.
Methods in Molecular Biology 1312:165 (2015)
Sera of tumor patients frequently contain autoantibodies to tumor associated antigens. Here we describe a miniaturized immunoblot platform allowing us to screen sera of patients for the presence of autoantibodies to ten autoantigens in parallel.