Functionality, growth and accelerated aging of tissue engineered living autologous vascular grafts.
Biomaterials 33(33):8277 (2012)
Living autologous tissue engineered vascular-grafts (TEVGs) with growth-capacity may overcome the limitations of contemporary artificial-prostheses. However, the multi-step in vitro production of TEVGs requires extensive ex vivo cell-manipulations with unknown effects on functionality and qualit...
3D microtissue formation of undifferentiated bone marrow mesenchymal stem cells leads to elevated apoptosis.
Tissue Engineering, Part A: Tissue Engineering 18(7-8):692 (2012)
Current implantation formats to deliver bone marrow-derived mesenchymal stem cells (MSCs) to the site of myocardial injury resulted only in limited cell retention and integration. As an alternative concept to single cell transplantation, we investigated the fate of cell tracker-labeled syngenic ...
Clemastine causes immune suppression through inhibition of extracellular signal-regulated kinase-dependent proinflammatory cytokines.
Journal of Allergy and Clinical Immunology 128(6):1286 (2011)
Antihistamines are considered safe and used worldwide against allergy, pruritus, nausea, and cough and as sleeping aids. Nonetheless, a growing number of reports suggest that antihistamines also have immunoregulatory functions.
We examined the extent and by what potential mechanisms histamine-1-...
Persistence of recipient-type endothelium after allogeneic hematopoietic stem cell transplantation.
Haematologica 96(1):119 (2011)
The possibility that allogeneic hematopoietic stem cell transplantation performed across the ABO blood group-barrier is associated with an increase of graft-versus-host disease, in particular endothelial damage, has not been elucidated so far. For this reason, we investigated the level of endoth...
Minimally-invasive implantation of living tissue engineered heart valves: a comprehensive approach from autologous vascular cells to stem cells.
Journal of the American College of Cardiology 56(6):510 (2010)
The aim of this study was to demonstrate the feasibility of combining the novel heart valve replacement technologies of: 1) tissue engineering; and 2) minimally-invasive implantation based on autologous cells and composite self-expandable biodegradable biomaterials.
Minimally-invasive valve repl...
A novel concept for scaffold-free vessel tissue engineering: self-assembly of microtissue building blocks.
Journal of Biotechnology 148(1):46 (2010)
Current scientific attempts to generate in vitro tissue-engineered living blood vessels (TEBVs) show substantial limitations, thereby preventing routine clinical use. In the present report, we describe a novel biotechnology concept to create living small diameter TEBV based exclusively on microt...
Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions.
The American Journal of Pathology 174(6):2310 (2009)
Phagocytosis is essential for the removal of photoreceptor debris following retinal injury. We used two mouse models, mice injected with green fluorescent protein-labeled bone marrow cells or green fluorescent protein-labeled microglia, to study the origin and activation patterns of phagocytic c...
Innate immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections.
PNAS 106(17):7107 (2009)
Neutrophils are the most abundant leukocytes in circulation and provide a primary innate immune defense function against bacterial pathogens before development of a specific immune response. These specialized phagocytes are short lived (12-24 hours) and continuously replenished from bone marrow....
Impaired antibody response causes persistence of prototypic T cell-contained virus.
PLoS Biology 7(4):e1000080 (2009)
CD8 T cells are recognized key players in control of persistent virus infections, but increasing evidence suggests that assistance from other immune mediators is also needed. Here, we investigated whether specific antibody responses contribute to control of lymphocytic choriomeningitis virus (LC...
Polyclonal and specific antibodies mediate protective immunity against enteric helminth infection.
Cell Host & Microbe 4(4):362 (2008)
Anti-helminth immunity involves CD4+ T cells, yet the precise effector mechanisms responsible for parasite killing or expulsion remain elusive. We now report an essential role for antibodies in mediating immunity against the enteric helminth Heligmosomoides polygyrus (Hp), a natural murine paras...
Aggravation of viral hepatitis by platelet-derived serotonin.
Nature Medicine 14(7):756 (2008)
More than 500 million people worldwide are persistently infected with hepatitis B virus or hepatitis C virus. Although both viruses are poorly cytopathic, persistence of either virus carries a risk of chronic liver inflammation, potentially resulting in liver steatosis, liver cirrhosis, end-stag...
Cryopreserved amniotic fluid-derived cells: a lifelong autologous fetal stem cell source for heart valve tissue engineering.
The Journal of heart valve disease 17(4):446 (2008)
Fetal stem cells represent a promising cell source for heart valve tissue engineering. In particular, amniotic fluid-derived cells (AFDC) have been shown to lead to autologous fetal-like heart valve tissues in vitro for pediatric application. In order to expand the versatility of these cells als...
PARP1 is required for adhesion molecule expression in atherogenesis.
Cardiovascular Research 78(1):158 (2008)
Atherosclerosis is the leading cause of death in Western societies and a chronic inflammatory disease. However, the key mediators linking recruitment of inflammatory cells to atherogenesis remain poorly defined. Poly(ADP-ribose) polymerase 1 (PARP1) is a nuclear enzyme, which plays a role in acu...
Differentiation of human follicular thyroid adenomas from carcinomas by gene expression profiling.
Oncology Reports 19(2):329 (2008)
It is difficult to distinguish benign from malignant follicular thyroid tumors by histological or cytological examination. The goal of this study was to reveal gene expression variations between benign and malignant follicular lesions of the thyroid gland. We investigated gene expression profile...
Extralymphatic virus sanctuaries as a consequence of potent T-cell activation.
Nature Medicine 13(11):1316 (2007)
T helper cells can support the functions of CD8(+) T cells against persistently infecting viruses such as murine lymphocytic choriomeningitis virus (LCMV), cytomegalovirus, hepatitis C virus and HIV. These viruses often resist complete elimination and remain detectable at sanctuary sites, such a...
Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source.
Circulation 116(11 Suppl):I64 (2007)
A novel concept providing prenatally tissue engineered human autologous heart valves based on routinely obtained fetal amniotic fluid progenitors as single cell source is introduced.
Fetal human amniotic progenitors were isolated from routinely sampled amniotic fluid and sorted using CD133 magne...
MyD88 protects from lethal encephalitis during infection with vesicular stomatitis virus.
European Journal of Immunology 37(9):2434 (2007)
MyD88 is a key adaptor molecule in innate resistance, engaged in most Toll-like receptor, as well as IL-1 and IL-18, signalling. Here, we analyzed the role of MyD88 in innate resistance during infection with vesicular stomatitis virus (VSV) using myd88(-/-) mice. We found an increased susceptibi...
Is EGFR a moving target during radiotherapy of carcinoma of the uterine cervix?
Gynecologic Oncology 106(2):394 (2007)
The epidermal growth factor receptor (EGFR) is frequently overexpressed in uterine cervix carcinoma. The role of the pre-treatment EGFR expression levels and the changes of expression induced by ionizing radiation (IR) have not been conclusively defined.
The staining intensity (SI) and labeling ...
Vesicular stomatitis virus glycoprotein displaying retrovirus-like particles induce a type I IFN receptor-dependent switch to neutralizing IgG antibodies.
Journal of Immunology 178(9):5839 (2007)
Vesicular stomatitis virus (VSV) infection rapidly induces IFN-alphabeta that confers initial survival, whereas long-term protection is mediated by neutralizing IgG responses. Because coadministration of IFN-alphabeta can enhance Ab responses against soluble Ags, we addressed whether virus-induc...
Local recurrence model of malignant pleural mesothelioma for investigation of intrapleural treatment.
European Journal of Cardio-thoracic Surgery 31(5):773 (2007)
Local recurrence remains a major problem in the treatment of malignant pleural mesothelioma. The aim of the underlying study was to establish a standardised local recurrence model in rats which enables to study different intrapleural therapies.
Fifty microlitre containing 1 x 10(6) cells of a sy...