1. Discovery of oxazolo[4,5-b]pyridines and related heterocyclic analogs as novel SIRT1 activators.

    Bioorganic & Medicinal Chemistry Letters 19(8):2350 (2009) PMID 19303289

    SIRT1 is an NAD(+)-dependent protein deacetylase that appears to produce beneficial effects on metabolic parameters such as glucose and insulin homeostasis. Activation of SIRT1 by resveratrol (1) has been shown to modulate insulin resistance, increase mitochondrial content and prolong survival i...
  2. Discovery of oxazolo[4,5-b]pyridines and related heterocyclic analogs as novel SIRT1 activators.

    Bioorganic & Medicinal Chemistry Letters 19(8):2350 (2009) PMID 19303289

    SIRT1 is an NAD(+)-dependent protein deacetylase that appears to produce beneficial effects on metabolic parameters such as glucose and insulin homeostasis. Activation of SIRT1 by resveratrol (1) has been shown to modulate insulin resistance, increase mitochondrial content and prolong survival i...
  3. Discovery of oxazolo[4,5-b]pyridines and related heterocyclic analogs as novel SIRT1 activators

    Bioorganic & Medicinal Chemistry Letters 19(8):2350 (2009) PMID 19303289

    The identification and SAR of novel small molecule activators of SIRT1, which are structurally distinct and more potent than resveratrol ( 1), are described.
  4. Small molecule activators of SIRT1 replicate signaling pathways triggered by calorie restriction in vivo.

    BMC Systems Biology 3:31 (2009) PMID 19284563 PMCID PMC2660283

    Calorie restriction (CR) produces a number of health benefits and ameliorates diseases of aging such as type 2 diabetes. The components of the pathways downstream of CR may provide intervention points for developing therapeutics for treating diseases of aging. The NAD+-dependent protein deacetyl...
  5. Discovery of oxazolo[4,5-b]pyridines and related heterocyclic analogs as novel SIRT1 activators

    Bioorganic & Medicinal Chemistry Letters 19(8):2350 (2009)

    The identification and SAR of novel small molecule activators of SIRT1, which are structurally distinct and more potent than resveratrol ( 1), are described.
  6. Small molecule activators of SIRT1 replicate signaling pathways triggered by calorie restriction in vivo.

    BMC Systems Biology 3:31 (2009) PMID 19284563 PMCID PMC2660283

    Calorie restriction (CR) produces a number of health benefits and ameliorates diseases of aging such as type 2 diabetes. The components of the pathways downstream of CR may provide intervention points for developing therapeutics for treating diseases of aging. The NAD+-dependent protein deacetyl...
  7. Small molecule activators of SIRT1 as therapeutics for the treatment of type 2 diabetes.

    Nature 450(7170):712 (2007) PMID 18046409 PMCID PMC2753457

    Calorie restriction extends lifespan and produces a metabolic profile desirable for treating diseases of ageing such as type 2 diabetes. SIRT1, an NAD+-dependent deacetylase, is a principal modulator of pathways downstream of calorie restriction that produce beneficial effects on glucose homeost...
  8. Fluorescent cardiac imaging: a novel intraoperative method for quantitative assessment of myocardial perfusion during graded coronary artery stenosis.

    Circulation 116(9):1007 (2007) PMID 17684152

    The purpose of the present study was to examine whether the effect of coronary stenoses of variable severity on myocardial perfusion can be quantitatively assessed in vivo by analysis of fluorescent cardiac imaging (FCI) compared with the gold standard, the fluorescent microsphere method. FCI is...
  9. Fluorescent cardiac imaging: a novel intraoperative method for quantitative assessment of myocardial perfusion during graded coronary artery stenosis.

    Circulation 116(9):1007 (2007) PMID 17684152

    The purpose of the present study was to examine whether the effect of coronary stenoses of variable severity on myocardial perfusion can be quantitatively assessed in vivo by analysis of fluorescent cardiac imaging (FCI) compared with the gold standard, the fluorescent microsphere method. FCI is...
  10. Structural determinants for inhibitor specificity and selectivity in PDE2A using the wheat germ in vitro translation system.

    Biochemistry (Washington) 44(23):8312 (2005) PMID 15938621

    Phosphodiesterases (PDEs) modulate signaling by cyclic nucleotides in diverse processes such as cardiac contractility, platelet aggregation, lipolysis, glycogenolysis, and smooth muscle contraction. Cyclic guanosine monophosphate (cGMP) stimulated human phosphodiesterase 2 (PDE2) is expressed ma...
  11. Structural determinants for inhibitor specificity and selectivity in PDE2A using the wheat germ in vitro translation system.

    Biochemistry (Washington) 44(23):8312 (2005) PMID 15938621

    Phosphodiesterases (PDEs) modulate signaling by cyclic nucleotides in diverse processes such as cardiac contractility, platelet aggregation, lipolysis, glycogenolysis, and smooth muscle contraction. Cyclic guanosine monophosphate (cGMP) stimulated human phosphodiesterase 2 (PDE2) is expressed ma...
  12. Examining reactivity and specificity of cytochrome c peroxidase by using combinatorial mutagenesis.

    ChemBioChem 3(11):1097 (2002) PMID 12404635

    Combinatorial mutagenesis was used to investigate the role of three key residues in cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae, Arg48, Trp51, and Trp191, in control of the reactivity and selectivity of the heme-containing enzyme. Libraries were prepared by randomization of these...
  13. Examining reactivity and specificity of cytochrome c peroxidase by using combinatorial mutagenesis.

    ChemBioChem 3(11):1097 (2002) PMID 12404635

    Combinatorial mutagenesis was used to investigate the role of three key residues in cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae, Arg48, Trp51, and Trp191, in control of the reactivity and selectivity of the heme-containing enzyme. Libraries were prepared by randomization of these...
  14. Near-infrared fluorescence coronary angiography: a new noninvasive technology for intraoperative graft patency control.

    Heart Surgery Forum 5(4):364 (2002) PMID 12538119

    Intraoperative graft patency verification is of major clinical importance for quality control after coronary artery bypass grafting (CABG), especially if surgery is performed on the beating heart. This is one of the first reports of fluorescence coronary angiography (FCA) using the dye indocyani...
  15. Imaging of Perfusion with Indocyanine Green Fluorescence a Promising Approach

    Medical Photonics 17(1):31 (2002)

    Intraoperative graft patency verification is of major clinical importance for quality control after coronary artery bypass grafting (CABG). The purpose of this study was assessment of graft patency and stenosis of variable severity by fluorescence angiography (FA) using indocyanine green (I...
  16. Imaging of Perfusion with Indocyanine Green Fluorescence a Promising Approach

    Medical Photonics 17(1):31 (2002)

    Intraoperative graft patency verification is of major clinical importance for quality control after coronary artery bypass grafting (CABG). The purpose of this study was assessment of graft patency and stenosis of variable severity by fluorescence angiography (FA) using indocyanine green (I...
  17. Near-infrared fluorescence coronary angiography: a new noninvasive technology for intraoperative graft patency control.

    Heart Surgery Forum 5(4):364 (2002) PMID 12538119

    Intraoperative graft patency verification is of major clinical importance for quality control after coronary artery bypass grafting (CABG), especially if surgery is performed on the beating heart. This is one of the first reports of fluorescence coronary angiography (FCA) using the dye indocyani...
  18. Changing the Substrate Specificity of CytochromecPeroxidase Using Directed Evolution

    Biochemical and Biophysical Research Communicat... 286(1):126 (2001)

    Cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae was subjected to directed molecular evolution to generate mutants with increased activity against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Using a combination of DNA shuffling and saturation mutagenesis, ...
  19. Changing the Substrate Specificity of CytochromecPeroxidase Using Directed Evolution

    Biochemical and Biophysical Research Communicat... 286(1):126 (2001)

    Cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae was subjected to directed molecular evolution to generate mutants with increased activity against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Using a combination of DNA shuffling and saturation muta...